In Vivo Absolute Quantification for Mouse Muscle Metabolites Using an Inductively Coupled Synthetic Signal Injection Method and Newly Developed (1) H/(31) P Dual Tuned Probe

Overview

Journal J Magn Reson Imaging

Date 2014 Jan 28

PMID 24464912

Authors

Donghoon Lee

Kenneth Marro

Mark Mathis

Eric Shankland

Cecil Hayes

Affiliations

Soon will be listed here.

Abstract

Purpose: To obtain robust estimates of (31) P metabolite content in mouse skeletal muscles using our recently developed MR absolute quantification method and a custom-built (1) H/(31) P dual tuned radiofrequency (RF) coil optimized for mouse leg.

Materials And Methods: We designed and fabricated a probe consisting of two dual tuned (1) H/(31) P solenoid coils: one leg was inserted to each solenoid. The mouse leg volume coil was incorporated with injector coils for MR absolute quantification. The absolute quantification method uses a synthetic reference signal injection approach and solves several challenges in MR absolute quantification including changes of coil loading and receiver gains.

Results: The (1) H/(31) P dual tuned probe was composed of two separate solenoid coils, one for each leg, to increase coil filling factors and signal-to-noise ratio. Each solenoid was equipped with a second coil to allow injection of reference signals. (31) P metabolite concentrations determined for normal mice were well within the expected range reported in the literature.

Conclusion: We developed an RF probe and an absolute quantification approach adapted for mouse skeletal muscle.

References

Naressi A, Couturier C, Devos J, Janssen M, Mangeat C, de Beer R . Java-based graphical user interface for the MRUI quantitation package. MAGMA. 2001; 12(2-3):141-52. DOI: 10.1007/BF02668096. View

Jansen J, Backes W, Nicolay K, Kooi M . 1H MR spectroscopy of the brain: absolute quantification of metabolites. Radiology. 2006; 240(2):318-32. DOI: 10.1148/radiol.2402050314. View

Vanhamme , van den Boogaart A , Van Huffel S . Improved method for accurate and efficient quantification of MRS data with use of prior knowledge . J Magn Reson. 1998; 129(1):35-43. DOI: 10.1006/jmre.1997.1244. View

Hazlewood C, Chang D, Nichols B, Woessner D . Nuclear magnetic resonance transverse relaxation times of water protons in skeletal muscle. Biophys J. 1974; 14(8):583-606. PMC: 1334554. DOI: 10.1016/S0006-3495(74)85937-0. View

Chen X, Pavan M, Heinzer-Schweizer S, Boesiger P, Henning A . Optically transmitted and inductively coupled electric reference to access in vivo concentrations for quantitative proton-decoupled ¹³C magnetic resonance spectroscopy. Magn Reson Med. 2011; 67(1):1-7. DOI: 10.1002/mrm.23110. View

Lee D, Marro K, Shankland E, Mathis M . Quantitative 19F imaging using inductively coupled reference signal injection. Magn Reson Med. 2010; 63(3):570-3. PMC: 2841308. DOI: 10.1002/mrm.22298. View

Roth S, Martel G, Rogers M . Muscle biopsy and muscle fiber hypercontraction: a brief review. Eur J Appl Physiol. 2001; 83(4 -5):239-45. DOI: 10.1007/s004210000287. View

Naressi A, Couturier C, Castang I, de Beer R, Graveron-Demilly D . Java-based graphical user interface for MRUI, a software package for quantitation of in vivo/medical magnetic resonance spectroscopy signals. Comput Biol Med. 2001; 31(4):269-86. DOI: 10.1016/s0010-4825(01)00006-3. View

Filosto M, Tonin P, Vattemi G, Bertolasi L, Simonati A, Rizzuto N . The role of muscle biopsy in investigating isolated muscle pain. Neurology. 2007; 68(3):181-6. DOI: 10.1212/01.wnl.0000252252.29532.cc. View

10.

Grady R, Teng H, Nichol M, Cunningham J, WILKINSON R, Sanes J . Skeletal and cardiac myopathies in mice lacking utrophin and dystrophin: a model for Duchenne muscular dystrophy. Cell. 1997; 90(4):729-38. DOI: 10.1016/s0092-8674(00)80533-4. View

11.

Najim E, Grivet J . A double-tuned probe for metabolic NMR studies. Magn Reson Med. 1992; 23(2):367-71. DOI: 10.1002/mrm.1910230216. View

12.

Heerschap A, Sommers M, in t Zandt H, Renema W, Veltien A, Klomp D . Nuclear magnetic resonance in laboratory animals. Methods Enzymol. 2004; 385:41-63. DOI: 10.1016/S0076-6879(04)85003-1. View

13.

Siegel M, Kruse S, Knowels G, Salmon A, Beyer R, Xie H . Reduced coupling of oxidative phosphorylation in vivo precedes electron transport chain defects due to mild oxidative stress in mice. PLoS One. 2011; 6(11):e26963. PMC: 3222658. DOI: 10.1371/journal.pone.0026963. View

14.

Barantin L, Le Pape A, Akoka S . A new method for absolute quantitation of MRS metabolites. Magn Reson Med. 1997; 38(2):179-82. DOI: 10.1002/mrm.1910380203. View

15.

Bulfield G, Siller W, Wight P, Moore K . X chromosome-linked muscular dystrophy (mdx) in the mouse. Proc Natl Acad Sci U S A. 1984; 81(4):1189-92. PMC: 344791. DOI: 10.1073/pnas.81.4.1189. View

16.

Deconinck A, Rafael J, Skinner J, Brown S, Potter A, METZINGER L . Utrophin-dystrophin-deficient mice as a model for Duchenne muscular dystrophy. Cell. 1997; 90(4):717-27. DOI: 10.1016/s0092-8674(00)80532-2. View

17.

Marro K, Lee D, Shankland E, Mathis C, Hayes C, Friedman S . Quantitative in vivo magnetic resonance spectroscopy using synthetic signal injection. PLoS One. 2011; 5(12):e15166. PMC: 3010995. DOI: 10.1371/journal.pone.0015166. View

18.

Chahin N, Engel A . Correlation of muscle biopsy, clinical course, and outcome in PM and sporadic IBM. Neurology. 2007; 70(6):418-24. DOI: 10.1212/01.wnl.0000277527.69388.fe. View

19.

Salinero-Paniagua E, Esteban-Garcia A, Traba A, Palencia-Herrejon E, Garzo-Fernandez C, Prieto-Montalvo J . [Modified aspiration needle muscular biopsy in neuromuscular diseases. Use and advantages over open surgical biopsy]. Rev Neurol. 2004; 39(9):821-5. View

20.

Masad I, Grant S . A retunable surface coil for high field 31P and 1H magnetic resonance evaluations of the living mouse leg. Physiol Meas. 2011; 32(8):1061-81. DOI: 10.1088/0967-3334/32/8/005. View