Castration-induced Changes in MicroRNA Expression Profiles in Subcutaneous Adipose Tissue of Male Pigs

Overview

Journal J Appl Genet

Publisher Springer

Specialty Genetics

Date 2014 Jan 28

PMID 24464334

Citations 9

Authors

Zhaowei Cai

Lifan Zhang

Minli Chen

Xiaoling Jiang

Ningying Xu

Affiliations

Soon will be listed here.

Abstract

MicroRNAs (miRNAs) are class of molecular regulators found to participate in numerous biological processes, such as adipogenesis and obesity in mammals. To determine the roles of miRNAs involved in castration-induced body fatness, we investigated the different miRNA expression patterns in subcutaneous adipose tissue between intact and castrated male pigs. Our results showed that castration led to decrease serum testosterone but increase serum Leptin levels (P < 0.01). Moreover, castration also increased adipocyte size, body fat content and backfat thickness in male pigs (P < 0.01). Meanwhile, miRNA expression profiles in adipose tissue were changed by castration, and 18 miRNAs were considered as the differentially expressed candidates between intact and castrated male pigs. Furthermore, functional analysis indicated that the differential expressed miRNAs and their target genes are involved in the regulation of fatty acid metabolism. In brief, our present study provides a comprehensive view on how miRNAs works in subcutaneous adipose tissue with castration. These results suggested that miRNAs might play an important role in the castration-induced fat deposition in male pigs.

Citing Articles

Integrated Analysis of the ceRNA Network and M-7474 Function in Testosterone-Mediated Fat Deposition in Pigs.

Liu X, Bai Y, Cui R, He S, Ling Y, Wu C Genes (Basel). 2022; 13(4).

PMID: 35456474 PMC: 9032878. DOI: 10.3390/genes13040668.

The Novel-miR-659/ Interaction Regulates Fat Deposition in Castrated Male Pigs.

Xiao L, Xu Q, Liu X, Chan S, Luo Y, He S Animals (Basel). 2022; 12(8).

PMID: 35454191 PMC: 9031235. DOI: 10.3390/ani12080944.

miR-F4-C12 Functions on the Regulation of Adipose Accumulation by Targeting PIK3R1 in Castrated Male Pigs.

Xu Q, Chen J, Liu X, Luo Y, Wang T, Fang M Animals (Basel). 2021; 11(11).

PMID: 34827785 PMC: 8614499. DOI: 10.3390/ani11113053.

Adipose Tissue Gene Expression of Entire Male, Immunocastrated and Surgically Castrated Pigs.

Poklukar K, candek-Potokar M, Vrecl M, Batorek-Lukac N, Fazarinc G, Kress K Int J Mol Sci. 2021; 22(4).

PMID: 33578947 PMC: 7916650. DOI: 10.3390/ijms22041768.

LncRNA IMFlnc1 promotes porcine intramuscular adipocyte adipogenesis by sponging miR-199a-5p to up-regulate CAV-1.

Wang J, Chen M, Chen J, Ren Q, Zhang J, Cao H BMC Mol Cell Biol. 2020; 21(1):77.

PMID: 33148167 PMC: 7640402. DOI: 10.1186/s12860-020-00324-8.

References

Lhakhang T, Chaudhry M . Current approaches to micro-RNA analysis and target gene prediction. J Appl Genet. 2011; 53(2):149-58. DOI: 10.1007/s13353-011-0060-2. View

Levadoux E, Morio B, Montaurier C, Puissant V, Boirie Y, Fellmann N . Reduced whole-body fat oxidation in women and in the elderly. Int J Obes Relat Metab Disord. 2001; 25(1):39-44. DOI: 10.1038/sj.ijo.0801530. View

Kajimoto K, Naraba H, Iwai N . MicroRNA and 3T3-L1 pre-adipocyte differentiation. RNA. 2006; 12(9):1626-32. PMC: 1557704. DOI: 10.1261/rna.7228806. View

Chen C, Deng B, Qiao M, Zheng R, Chai J, Ding Y . Solexa sequencing identification of conserved and novel microRNAs in backfat of Large White and Chinese Meishan pigs. PLoS One. 2012; 7(2):e31426. PMC: 3280305. DOI: 10.1371/journal.pone.0031426. View

Andersen D, Jensen C, Schneider M, Nossent A, Eskildsen T, Hansen J . MicroRNA-15a fine-tunes the level of Delta-like 1 homolog (DLK1) in proliferating 3T3-L1 preadipocytes. Exp Cell Res. 2010; 316(10):1681-91. DOI: 10.1016/j.yexcr.2010.04.002. View

Esau C, Kang X, Peralta E, Hanson E, Marcusson E, Ravichandran L . MicroRNA-143 regulates adipocyte differentiation. J Biol Chem. 2004; 279(50):52361-5. DOI: 10.1074/jbc.C400438200. View

Li M, Bjorntorp P . Effects of testosterone on triglyceride uptake and mobilization in different adipose tissues in male rats in vivo. Obes Res. 1995; 3(2):113-9. DOI: 10.1002/j.1550-8528.1995.tb00128.x. View

Gerin I, Bommer G, McCoin C, Sousa K, Krishnan V, MacDougald O . Roles for miRNA-378/378* in adipocyte gene expression and lipogenesis. Am J Physiol Endocrinol Metab. 2010; 299(2):E198-206. PMC: 2928515. DOI: 10.1152/ajpendo.00179.2010. View

Mashek D, Li L, Coleman R . Rat long-chain acyl-CoA synthetase mRNA, protein, and activity vary in tissue distribution and in response to diet. J Lipid Res. 2006; 47(9):2004-10. DOI: 10.1194/jlr.M600150-JLR200. View

10.

Kim Y, Hwang S, Bae Y, Jung J . MiR-21 regulates adipogenic differentiation through the modulation of TGF-beta signaling in mesenchymal stem cells derived from human adipose tissue. Stem Cells. 2009; 27(12):3093-102. DOI: 10.1002/stem.235. View

11.

Kim S, Kim A, Lee H, Son Y, Lee G, Lee J . miR-27a is a negative regulator of adipocyte differentiation via suppressing PPARgamma expression. Biochem Biophys Res Commun. 2010; 392(3):323-8. DOI: 10.1016/j.bbrc.2010.01.012. View

12.

Christoffersen B, Gade L, Golozoubova V, Svendsen O, Raun K . Influence of castration-induced testosterone and estradiol deficiency on obesity and glucose metabolism in male Göttingen minipigs. Steroids. 2010; 75(10):676-84. DOI: 10.1016/j.steroids.2010.04.004. View

13.

Li G, Li Y, Li X, Ning X, Li M, Yang G . MicroRNA identity and abundance in developing swine adipose tissue as determined by Solexa sequencing. J Cell Biochem. 2011; 112(5):1318-28. DOI: 10.1002/jcb.23045. View

14.

Grimson A, Farh K, Johnston W, Garrett-Engele P, Lim L, Bartel D . MicroRNA targeting specificity in mammals: determinants beyond seed pairing. Mol Cell. 2007; 27(1):91-105. PMC: 3800283. DOI: 10.1016/j.molcel.2007.06.017. View

15.

Wang C, Su Z, Sanai N, Xue X, Lu L, Chen Y . microRNA expression profile and differentially-expressed genes in prolactinomas following bromocriptine treatment. Oncol Rep. 2012; 27(5):1312-20. DOI: 10.3892/or.2012.1690. View

16.

Donkor J, Sparks L, Xie H, Smith S, Reue K . Adipose tissue lipin-1 expression is correlated with peroxisome proliferator-activated receptor alpha gene expression and insulin sensitivity in healthy young men. J Clin Endocrinol Metab. 2007; 93(1):233-9. PMC: 2190746. DOI: 10.1210/jc.2007-1535. View

17.

Marsh E, Lin Z, Yin P, Milad M, Chakravarti D, Bulun S . Differential expression of microRNA species in human uterine leiomyoma versus normal myometrium. Fertil Steril. 2007; 89(6):1771-6. PMC: 2773156. DOI: 10.1016/j.fertnstert.2007.05.074. View

18.

Cline M, Smoot M, Cerami E, Kuchinsky A, Landys N, Workman C . Integration of biological networks and gene expression data using Cytoscape. Nat Protoc. 2007; 2(10):2366-82. PMC: 3685583. DOI: 10.1038/nprot.2007.324. View

19.

Keller P, Gburcik V, Petrovic N, Gallagher I, Nedergaard J, Cannon B . Gene-chip studies of adipogenesis-regulated microRNAs in mouse primary adipocytes and human obesity. BMC Endocr Disord. 2011; 11:7. PMC: 3070678. DOI: 10.1186/1472-6823-11-7. View

20.

Anderson L, McTernan P, Barnett A, Kumar S . The effects of androgens and estrogens on preadipocyte proliferation in human adipose tissue: influence of gender and site. J Clin Endocrinol Metab. 2001; 86(10):5045-51. DOI: 10.1210/jcem.86.10.7955. View