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A Simple Method of Fast Extracellular Solution Exchange for the Study of Whole-cell or Single Channel Currents Using Patch-clamp Technique

Overview
Journal Pflugers Arch
Specialty Physiology
Date 1987 Oct 1
PMID 2446257
Citations 11
Authors
S Hering
D J Beech
T B Bolton
Affiliations
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Abstract

A new concentration-jump technique was devised for the rapid application of drugs to single, isolated cells attached to the base of the experimental chamber while recording from them with patch-clamp technique. Cells were placed in a micro-drop (less than 0.1 microliter) in a small inner bath which was separated from an outer bath by a ring of "Sylgard" polymer. Stable whole-cell recordings were made in the micro-drop and rapid solution exchange took place when a much larger volume of test solution from the outer bath was flooded over the Sylgard ring and mixed with the micro-drop. Complete equilibration occurred within less than 10 ms.

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