Ginkgo Biloba: a Natural Reducing Agent for the Synthesis of Cytocompatible Graphene

Overview

Journal Int J Nanomedicine

Publisher Dove Medical Press

Specialty Biotechnology

Date 2014 Jan 24

PMID 24453487

Citations 20

Authors

Sangiliyandi Gurunathan

Jae Woong Han

Jung Hyun Park

Vasuki Eppakayala

Jin-Hoi Kim

Affiliations

Soon will be listed here.

Abstract

Background: Graphene is a novel two-dimensional planar nanocomposite material consisting of rings of carbon atoms with a hexagonal lattice structure. Graphene exhibits unique physical, chemical, mechanical, electrical, elasticity, and cytocompatible properties that lead to many potential biomedical applications. Nevertheless, the water-insoluble property of graphene restricts its application in various aspects of biomedical fields. Therefore, the objective of this work was to find a novel biological approach for an efficient method to synthesize water-soluble and cytocompatible graphene using Ginkgo biloba extract (GbE) as a reducing and stabilizing agent. In addition, we investigated the biocompatibility effects of graphene in MDA-MB-231 human breast cancer cells.

Materials And Methods: Synthesized graphene oxide (GO) and GbE-reduced GO (Gb-rGO) were characterized using various sequences of techniques: ultraviolet-visible (UV-vis) spectroscopy, Fourier-transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), scanning electron microscopy (SEM), atomic force microscopy (AFM), and Raman spectroscopy. Biocompatibility of GO and Gb-rGO was assessed in human breast cancer cells using a series of assays, including cell viability, apoptosis, and alkaline phosphatase (ALP) activity.

Results: The successful synthesis of graphene was confirmed by UV-vis spectroscopy and FTIR. DLS analysis was performed to determine the average size of GO and Gb-rGO. X-ray diffraction studies confirmed the crystalline nature of graphene. SEM was used to investigate the surface morphologies of GO and Gb-rGO. AFM was employed to investigate the morphologies of prepared graphene and the height profile of GO and Gb-rGO. The formation of defects in Gb-rGO was confirmed by Raman spectroscopy. The biocompatibility of the prepared GO and Gb-rGO was investigated using a water-soluble tetrazolium 8 assay on human breast cancer cells. GO exhibited a dose-dependent toxicity, whereas Gb-rGO-treated cells showed significant biocompatibility and increased ALP activity compared to GO.

Conclusion: In this work, a nontoxic natural reducing agent of GbE was used to prepare soluble graphene. The as-prepared Gb-rGO showed significant biocompatibility with human cancer cells. This simple, cost-effective, and green procedure offers an alternative route for large-scale production of rGO, and could be used for various biomedical applications, such as tissue engineering, drug delivery, biosensing, and molecular imaging.

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References

Mohanty N, Berry V . Graphene-based single-bacterium resolution biodevice and DNA transistor: interfacing graphene derivatives with nanoscale and microscale biocomponents. Nano Lett. 2009; 8(12):4469-76. DOI: 10.1021/nl802412n. View

Sanchez V, Jachak A, Hurt R, Kane A . Biological interactions of graphene-family nanomaterials: an interdisciplinary review. Chem Res Toxicol. 2011; 25(1):15-34. PMC: 3259226. DOI: 10.1021/tx200339h. View

Gurunathan S, Han J, Eppakayala V, Kim J . Biocompatibility of microbially reduced graphene oxide in primary mouse embryonic fibroblast cells. Colloids Surf B Biointerfaces. 2013; 105:58-66. DOI: 10.1016/j.colsurfb.2012.12.036. View

Rao C, Sood A, Subrahmanyam K, Govindaraj A . Graphene: the new two-dimensional nanomaterial. Angew Chem Int Ed Engl. 2009; 48(42):7752-77. DOI: 10.1002/anie.200901678. View

Chen G, Hwang S, Su H, Kuo C, Luo W, Lo K . Defective antiviral responses of induced pluripotent stem cells to baculoviral vector transduction. J Virol. 2012; 86(15):8041-9. PMC: 3421682. DOI: 10.1128/JVI.00808-12. View

Lu C, Zhu C, Li J, Liu J, Chen X, Yang H . Using graphene to protect DNA from cleavage during cellular delivery. Chem Commun (Camb). 2010; 46(18):3116-8. DOI: 10.1039/b926893f. View

Gurunathan S, Han J, Eppakayala V, Kim J . Microbial reduction of graphene oxide by Escherichia coli: a green chemistry approach. Colloids Surf B Biointerfaces. 2012; 102:772-7. DOI: 10.1016/j.colsurfb.2012.09.011. View

Gotoh Y, Hiraiwa K, Nagayama M . In vitro mineralization of osteoblastic cells derived from human bone. Bone Miner. 1990; 8(3):239-50. DOI: 10.1016/0169-6009(90)90109-s. View

Liao K, Lin Y, Macosko C, Haynes C . Cytotoxicity of graphene oxide and graphene in human erythrocytes and skin fibroblasts. ACS Appl Mater Interfaces. 2011; 3(7):2607-15. DOI: 10.1021/am200428v. View

10.

Park S, An J, Jung I, Piner R, An S, Li X . Colloidal suspensions of highly reduced graphene oxide in a wide variety of organic solvents. Nano Lett. 2009; 9(4):1593-7. DOI: 10.1021/nl803798y. View

11.

Lee W, Lim C, Shi H, Tang L, Wang Y, Lim C . Origin of enhanced stem cell growth and differentiation on graphene and graphene oxide. ACS Nano. 2011; 5(9):7334-41. DOI: 10.1021/nn202190c. View

12.

Novoselov K, Geim A, Morozov S, Jiang D, Zhang Y, Dubonos S . Electric field effect in atomically thin carbon films. Science. 2004; 306(5696):666-9. DOI: 10.1126/science.1102896. View

13.

Zhang Y, Ali S, Dervishi E, Xu Y, Li Z, Casciano D . Cytotoxicity effects of graphene and single-wall carbon nanotubes in neural phaeochromocytoma-derived PC12 cells. ACS Nano. 2010; 4(6):3181-6. DOI: 10.1021/nn1007176. View

14.

Liu S, Zeng T, Hofmann M, Burcombe E, Wei J, Jiang R . Antibacterial activity of graphite, graphite oxide, graphene oxide, and reduced graphene oxide: membrane and oxidative stress. ACS Nano. 2011; 5(9):6971-80. DOI: 10.1021/nn202451x. View

15.

Akhavan O, Ghaderi E . Toxicity of graphene and graphene oxide nanowalls against bacteria. ACS Nano. 2010; 4(10):5731-6. DOI: 10.1021/nn101390x. View

16.

Wang Y, Shi Z, Yin J . Facile synthesis of soluble graphene via a green reduction of graphene oxide in tea solution and its biocomposites. ACS Appl Mater Interfaces. 2011; 3(4):1127-33. DOI: 10.1021/am1012613. View

17.

Sayes C, Liang F, Hudson J, Mendez J, Guo W, Beach J . Functionalization density dependence of single-walled carbon nanotubes cytotoxicity in vitro. Toxicol Lett. 2005; 161(2):135-42. DOI: 10.1016/j.toxlet.2005.08.011. View

18.

Nayak T, Andersen H, Makam V, Khaw C, Bae S, Xu X . Graphene for controlled and accelerated osteogenic differentiation of human mesenchymal stem cells. ACS Nano. 2011; 5(6):4670-8. DOI: 10.1021/nn200500h. View

19.

Feng L, Zhang S, Liu Z . Graphene based gene transfection. Nanoscale. 2011; 3(3):1252-7. DOI: 10.1039/c0nr00680g. View

20.

Wu Q, Xu Y, Yao Z, Liu A, Shi G . Supercapacitors based on flexible graphene/polyaniline nanofiber composite films. ACS Nano. 2010; 4(4):1963-70. DOI: 10.1021/nn1000035. View