Molecular Characterization and Expression of Type-I Interferon Gene in Labeo Rohita

Overview

Journal Mol Biol Rep

Specialty Molecular Biology

Date 2014 Jan 23

PMID 24449367

Citations 1

Authors

Janmejay Parhi

S C Mukherjee

Gopalkrishna Saxena

Lopamudra Sahoo

M Makesh

Affiliations

Soon will be listed here.

Abstract

Genes coding for type-I interferon (I-IFN) has been cloned from Labeo rohita, a commercially important and widely cultured fish in India and South East Asia. In the present study, full-length gene of I-IFN was amplified and sequenced. The sequence analysis revealed that I-IFN consists of 1,786 bp genomic sequence with four introns and five exons and an ORF of 546 bp encoding for a putative protein of 181 amino acids. The mature protein has a molecular weight of 18.97 kDa and consists of 158 amino acids and a signal peptide of 23 amino acids at the N terminus. The sequence carries I-IFN signature motif, one glycosylation site, two conserved cystine amino acids and other conserved amino acids. The sequence showed highest similarity to that of Cyprinus carpio (84%). In silico analysis of the rohu I-IFN protein was done using various bioinformatic tools. The constitutive expression of I-IFN gene was found to be more in spleen compared to gill and kidney in real time PCR assay. Expression of I-IFN increased about 20-fold in cultured kidney cell 2 h after induction with poly I:C and showed maximum expression at 8 h post-induction.

Citing Articles

Sensors of Infection: Viral Nucleic Acid PRRs in Fish.

Poynter S, Lisser G, Monjo A, DeWitte-Orr S Biology (Basel). 2015; 4(3):460-93.

PMID: 26184332 PMC: 4588145. DOI: 10.3390/biology4030460.

References

Stark G, Kerr I, Williams B, Silverman R, Schreiber R . How cells respond to interferons. Annu Rev Biochem. 1998; 67:227-64. DOI: 10.1146/annurev.biochem.67.1.227. View

Samuel C . Antiviral actions of interferons. Clin Microbiol Rev. 2001; 14(4):778-809, table of contents. PMC: 89003. DOI: 10.1128/CMR.14.4.778-809.2001. View

Pestka S, Krause C, Walter M . Interferons, interferon-like cytokines, and their receptors. Immunol Rev. 2004; 202:8-32. DOI: 10.1111/j.0105-2896.2004.00204.x. View

Zou J, Bird S, Truckle J, Bols N, Horne M, Secombes C . Identification and expression analysis of an IL-18 homologue and its alternatively spliced form in rainbow trout (Oncorhynchus mykiss). Eur J Biochem. 2004; 271(10):1913-23. DOI: 10.1111/j.1432-1033.2004.04101.x. View

Jensen I, Larsen R, Robertsen B . An antiviral state induced in Chinook salmon embryo cells (CHSE-214) by transfection with the double-stranded RNA poly I:C. Fish Shellfish Immunol. 2002; 13(5):367-78. DOI: 10.1006/fsim.2002.0412. View

Su J, Zhu Z, Wang Y . Molecular cloning, characterization and expression analysis of the PKZ gene in rare minnow Gobiocypris rarus. Fish Shellfish Immunol. 2008; 25(1-2):106-13. DOI: 10.1016/j.fsi.2008.03.006. View

Zhang Y, Jiang J, Chen Y, Zhu R, Shi Y, Zhang Q . The innate immune response to grass carp hemorrhagic virus (GCHV) in cultured Carassius auratus blastulae (CAB) cells. Dev Comp Immunol. 2006; 31(3):232-43. DOI: 10.1016/j.dci.2006.05.015. View

Long S, Wilson M, Bengten E, Bryan L, Clem L, Miller N . Identification of a cDNA encoding channel catfish interferon. Dev Comp Immunol. 2003; 28(2):97-111. DOI: 10.1016/s0145-305x(03)00122-8. View

Zou J, Yoshiura Y, Dijkstra J, Sakai M, Ototake M, Secombes C . Identification of an interferon gamma homologue in Fugu, Takifugu rubripes. Fish Shellfish Immunol. 2004; 17(4):403-9. DOI: 10.1016/j.fsi.2004.04.015. View

10.

Nygaard R, Husgard S, Sommer A, Leong J, Robertsen B . Induction of Mx protein by interferon and double-stranded RNA in salmonid cells. Fish Shellfish Immunol. 2000; 10(5):435-50. DOI: 10.1006/fsim.1999.0249. View

11.

Fellous M, Nir U, Wallach D, Merlin G, Rubinstein M, Revel M . Interferon-dependent induction of mRNA for the major histocompatibility antigens in human fibroblasts and lymphoblastoid cells. Proc Natl Acad Sci U S A. 1982; 79(10):3082-6. PMC: 346357. DOI: 10.1073/pnas.79.10.3082. View

12.

Kumar S, Nei M, Dudley J, Tamura K . MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform. 2008; 9(4):299-306. PMC: 2562624. DOI: 10.1093/bib/bbn017. View

13.

Marrack P, Kappler J, Mitchell T . Type I interferons keep activated T cells alive. J Exp Med. 1999; 189(3):521-30. PMC: 2192920. DOI: 10.1084/jem.189.3.521. View

14.

Kontsek P, Karayianni-Vasconcelos G, Kontsekova E . The human interferon system: characterization and classification after discovery of novel members. Acta Virol. 2004; 47(4):201-15. View

15.

Robertsen B, Bergan V, Rokenes T, Larsen R, Albuquerque A . Atlantic salmon interferon genes: cloning, sequence analysis, expression, and biological activity. J Interferon Cytokine Res. 2003; 23(10):601-12. DOI: 10.1089/107999003322485107. View

16.

Biron C, Nguyen K, Pien G, Cousens L, Salazar-Mather T . Natural killer cells in antiviral defense: function and regulation by innate cytokines. Annu Rev Immunol. 1999; 17:189-220. DOI: 10.1146/annurev.immunol.17.1.189. View

17.

Zou J, Tafalla C, Truckle J, Secombes C . Identification of a second group of type I IFNs in fish sheds light on IFN evolution in vertebrates. J Immunol. 2007; 179(6):3859-71. DOI: 10.4049/jimmunol.179.6.3859. View

18.

Altmann S, Mellon M, Distel D, Kim C . Molecular and functional analysis of an interferon gene from the zebrafish, Danio rerio. J Virol. 2003; 77(3):1992-2002. PMC: 140984. DOI: 10.1128/jvi.77.3.1992-2002.2003. View

19.

Bendtsen J, Nielsen H, von Heijne G, Brunak S . Improved prediction of signal peptides: SignalP 3.0. J Mol Biol. 2004; 340(4):783-95. DOI: 10.1016/j.jmb.2004.05.028. View

20.

Buelens C, Bartholome E, Amraoui Z, Boutriaux M, Salmon I, Thielemans K . Interleukin-3 and interferon beta cooperate to induce differentiation of monocytes into dendritic cells with potent helper T-cell stimulatory properties. Blood. 2002; 99(3):993-8. DOI: 10.1182/blood.v99.3.993. View