An Improved Colloidal Silver Staining Method of Protein Blots on Nitrocellulose Membranes

Overview

Journal Folia Biol (Praha)

Specialty Biology

Date 1987 Jan 1

PMID 2444470

Citations 8

Authors

A Kovarik

K Hlubinova

A Vrbenska

J Prachar

Affiliations

Soon will be listed here.

Abstract

A simple method of revealing proteins on nitrocellulose blots is described. It is based on high affinity of colloidal silver for proteins. The sensitivity is about the same as that of colloidal gold and it appears to be about 20 times higher than the presently used staining methods. The whole procedure is very simple and does not take more than 30 min.

Citing Articles

BmpA is a surface-exposed outer-membrane protein of Borrelia burgdorferi.

Bryksin A, Tomova A, Godfrey H, Cabello F FEMS Microbiol Lett. 2010; 309(1):77-83.

PMID: 20546313 PMC: 2913681. DOI: 10.1111/j.1574-6968.2010.02020.x.

Epigenetic reprogramming during wound healing: loss of polycomb-mediated silencing may enable upregulation of repair genes.

Shaw T, Martin P EMBO Rep. 2009; 10(8):881-6.

PMID: 19575012 PMC: 2726669. DOI: 10.1038/embor.2009.102.

Circumventing the effect of product toxicity: development of a novel two-stage production process for the lantibiotic gallidermin.

Valsesia G, Medaglia G, Held M, Minas W, Panke S Appl Environ Microbiol. 2006; 73(5):1635-45.

PMID: 17194841 PMC: 1828792. DOI: 10.1128/AEM.01969-06.

Borrelia burgdorferi binds to, invades, and colonizes native type I collagen lattices.

Zambrano M, Beklemisheva A, Bryksin A, Newman S, Cabello F Infect Immun. 2004; 72(6):3138-46.

PMID: 15155615 PMC: 415685. DOI: 10.1128/IAI.72.6.3138-3146.2004.

Localization of BmpA on the exposed outer membrane of Borrelia burgdorferi by monospecific anti-recombinant BmpA rabbit antibodies.

Shin J, Bryksin A, Godfrey H, Cabello F Infect Immun. 2004; 72(4):2280-7.

PMID: 15039353 PMC: 375190. DOI: 10.1128/IAI.72.4.2280-2287.2004.