The Size and Distribution of Polysaccharides During Their Synthesis Within the Membrane System of Maize Root Cells

Overview

Journal Planta

Specialty Biology

Date 2014 Jan 17

PMID 24430684

Citations 5

Authors

D J Bowles

D H Northcote

Affiliations

Soon will be listed here.

Abstract

The polymers contained within pellets rich in either dictyosomes or the endoplasmic reticulum have been investigated. These were detected by the incorporation of radioactivity from D-[U-(14)C]glucose into the sugars in the intact root. Three types of material were obtained 1) water-soluble 2) soluble in chloroform 3) insoluble. The relative proportions of each of these 3 fractions were different in the 2 membrane preparations. The dictyosome pellet contained a high proportion of water-soluble material, some of the water-soluble polysaccharides contained in both membrane preparations had a molecular weight greater than 40,000. These polymers resembled the pectins and xylans deposited in the cell wall. Some of the insoluble material could be rendered soluble by incubation with proteolytic enzymes and all of this from the dictyosome pellet had a molecular weight greater than 4,000 whereas most of that obtained from the endoplasmic reticulum had a molecular weight less than 4,000 and it did not contain fucose.

Citing Articles

Xylan synthetase activity in differentiated xylem cells of sycamore trees (Acer pseudoplatanus).

DAlessandro G, Northcote D Planta. 2013; 151(1):53-60.

PMID: 24301670 DOI: 10.1007/BF00384237.

Comparison between maize root cells and their respective regenerating protoplasts: wall polysaccharides.

Pilet P, Blaschek W, Senn A, Franz G Planta. 2013; 161(5):465-9.

PMID: 24253848 DOI: 10.1007/BF00394579.

The animal ether phospholipid platelet-activating factor stimulates acidification of the incubation medium by cultured soybean cells.

Scherer G, Nickel R Plant Cell Rep. 2013; 7(7):575-8.

PMID: 24240421 DOI: 10.1007/BF00272761.

Detection of root mucilage using an anti-fucose antibody.

Sinha Roy S, Mittra B, Sharma S, Das T, Babu C Ann Bot. 2002; 89(3):293-9.

PMID: 12096741 PMC: 4233819. DOI: 10.1093/aob/mcf040.

Some features of secretory systems in plants.

Juniper B, Gilchrist A, Robins R Histochem J. 1977; 9(5):659-80.

PMID: 908653 DOI: 10.1007/BF01002907.

References

BLIGH E, Dyer W . A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959; 37(8):911-7. DOI: 10.1139/o59-099. View

Villemez C, SWANSON A, HASSID W . Properties of a polygalacturonic acid-synthesizing enzyme system from Phaseolus aureus seedlings. Arch Biochem Biophys. 1966; 116(1):446-52. DOI: 10.1016/0003-9861(66)90051-8. View

Ray P . Regulation of beta-Glucan Synthetase Activity by Auxin in Pea Stem Tissue: I. Kinetic Aspects. Plant Physiol. 1973; 51(4):601-8. PMC: 366315. DOI: 10.1104/pp.51.4.601. View

Clark A, Villemez C . The Formation of beta, 1 --> 4 Glucan from UDP-alpha-d-Glucose Catalyzed by a Phaseolus aureus Enzyme. Plant Physiol. 1972; 50(3):371-4. PMC: 366144. DOI: 10.1104/pp.50.3.371. View

Kauss H, Glaser C . Carbohydrate-binding proteins from plant cell walls and their possible involvement in extension growth. FEBS Lett. 1974; 45(1):304-7. DOI: 10.1016/0014-5793(74)80867-7. View

Poste G, Allison A . Membrane fusion. Biochim Biophys Acta. 1973; 300(4):421-65. DOI: 10.1016/0304-4157(73)90015-4. View

Bowles D, Northcote D . The sites of synthesis and transport of extracellular polysaccharides in the root tissues of maize. Biochem J. 1972; 130(4):1133-45. PMC: 1174563. DOI: 10.1042/bj1301133. View

Kauss H, SWANSON A, Arnold R, Odzuck W . Biosynthesis of pectic substances. Localisation of enzymes and products in a lipid-membrane complex. Biochim Biophys Acta. 1969; 192(1):55-61. DOI: 10.1016/0304-4165(69)90009-9. View

Barrett A, Northcote D . APPLE FRUIT PECTIC SUBSTANCES. Biochem J. 1965; 94:617-27. PMC: 1206596. DOI: 10.1042/bj0940617. View

10.

Northcote D, Pickett-Heaps J . A function of the Golgi apparatus in polysaccharide synthesis and transport in the root-cap cells of wheat. Biochem J. 1966; 98(1):159-67. PMC: 1264809. DOI: 10.1042/bj0980159. View

11.

Hanke D, Northcote D . Cell wall formation by soybean callus protoplasts. J Cell Sci. 1974; 14(1):29-50. DOI: 10.1242/jcs.14.1.29. View

12.

Northcote D . Fine structure of cytoplasm in relation to synthesis and secretion in plant cells. Proc R Soc Lond B Biol Sci. 1969; 173(1030):21-30. DOI: 10.1098/rspb.1969.0033. View

13.

Pickett-Heaps J . Further observations on the Golgi apparatus and its functions in cells of the wheat seedling. J Ultrastruct Res. 1967; 18(3):287-303. DOI: 10.1016/s0022-5320(67)80119-9. View

14.

Wright K, Bowles D . Effects of hormones on the polysaccharide-synthesizing membrane systems of lettuce pith. J Cell Sci. 1974; 16(2):433-43. DOI: 10.1242/jcs.16.2.433. View

15.

Bailey R, Kauss H . Extraction of hydroxyproline-containing proteins and pectic substances from cell walls of growing and non-growing mung bean hypocotyl segments. Planta. 2014; 119(3):233-45. DOI: 10.1007/BF00429047. View

16.

Kauss H, SWANSON A . Cooperation of enzymes responsible for polymerisation and methylation in pectin biosynthesis. Z Naturforsch B. 1969; 24(1):28-33. DOI: 10.1515/znb-1969-0109. View

17.

ASPINALL G, Molloy J, Craig J . Extracellular polysaccharides from suspension-cultured sycamore cells. Can J Biochem. 1969; 47(11):1063-70. DOI: 10.1139/o69-170. View

18.

Bowles D, Northcote D . The amounts and rates of export of polysaccharides found within the membrane system of maize root cells. Biochem J. 1974; 142(1):139-44. PMC: 1168219. DOI: 10.1042/bj1420139. View

19.

Harris P, Northcote D . Polysaccharide formation in plant Golgi bodies. Biochim Biophys Acta. 1971; 237(1):56-64. DOI: 10.1016/0304-4165(71)90029-8. View

20.

Spiro R . Glycoproteins. Annu Rev Biochem. 1970; 39:599-638. DOI: 10.1146/annurev.bi.39.070170.003123. View