Use of Halogenated Precursors to Define a Transcription Time Window After Treatment with Hypometabolizing Molecules

Overview

Journal Histochem Cell Biol

Publisher Springer

Specialties Biochemistry
Cell Biology

Date 2014 Jan 17

PMID 24429832

Citations 3

Authors

Alessandro Spedito

Barbara Cisterna

Manuela Malatesta

Marco Biggiogera

Affiliations

Soon will be listed here.

Abstract

A new method is proposed which combines the high spatial resolution of transmission electron microscopy with information on the dynamics of transcription. Incorporation of two different RNA precursors was used to define a time transcription window on cultured cells treated with hypometabolizing peptides which are known to modulate transcription. This procedure allows detecting a single fibril of newly synthesized RNA in the time range in which it is transcribed.

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