Cell Penetration to Nanofibrous Scaffolds: Forcespinning®, an Alternative Approach for Fabricating 3D Nanofibers

Overview

Journal Cell Adh Migr

Specialty Cell Biology

Date 2014 Jan 17

PMID 24429388

Citations 10

Authors

Michala Rampichova

Matej Buzgo

Jiri Chvojka

Eva Prosecka

Olga Kofronova

Evzen Amler

Affiliations

Soon will be listed here.

Abstract

Cell infiltration is a critical parameter for the successful development of 3D matrices for tissue engineering. Application of electrospun nanofibers in tissue engineering has recently attracted much attention. Notwithstanding several of their advantages, small pore size and small thickness of the electrospun layer limit their application for development of 3D scaffolds. Several methods for the pore size and/or electrospun layer thickness increase have been recently developed. Nevertheless, tissue engineering still needs emerging of either novel nanofiber-enriched composites or new techniques for 3D nanofiber fabrication. Forcespinning(®) seems to be a promising alternative. The potential of the Forcespinning(®) method is illustrated in preliminary experiment with mesenchymal stem cells.

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References

Li D, Ouyang G, McCann J, Xia Y . Collecting electrospun nanofibers with patterned electrodes. Nano Lett. 2005; 5(5):913-6. DOI: 10.1021/nl0504235. View

Rampichova M, Chvojka J, Buzgo M, Prosecka E, Mikes P, Vyslouzilova L . Elastic three-dimensional poly (ε-caprolactone) nanofibre scaffold enhances migration, proliferation and osteogenic differentiation of mesenchymal stem cells. Cell Prolif. 2012; 46(1):23-37. PMC: 6496386. DOI: 10.1111/cpr.12001. View

Sill T, von Recum H . Electrospinning: applications in drug delivery and tissue engineering. Biomaterials. 2008; 29(13):1989-2006. DOI: 10.1016/j.biomaterials.2008.01.011. View

Shabani I, Haddadi-Asl V, Seyedjafari E, Soleimani M . Cellular infiltration on nanofibrous scaffolds using a modified electrospinning technique. Biochem Biophys Res Commun. 2012; 423(1):50-4. DOI: 10.1016/j.bbrc.2012.05.069. View

Blakeney B, Tambralli A, Anderson J, Andukuri A, Lim D, Dean D . Cell infiltration and growth in a low density, uncompressed three-dimensional electrospun nanofibrous scaffold. Biomaterials. 2010; 32(6):1583-90. PMC: 3023580. DOI: 10.1016/j.biomaterials.2010.10.056. View

Nam J, Huang Y, Agarwal S, Lannutti J . Improved cellular infiltration in electrospun fiber via engineered porosity. Tissue Eng. 2007; 13(9):2249-57. PMC: 4948987. DOI: 10.1089/ten.2006.0306. View

Zhu X, Cui W, Li X, Jin Y . Electrospun fibrous mats with high porosity as potential scaffolds for skin tissue engineering. Biomacromolecules. 2008; 9(7):1795-801. DOI: 10.1021/bm800476u. View

Sundararaghavan H, Metter R, Burdick J . Electrospun fibrous scaffolds with multiscale and photopatterned porosity. Macromol Biosci. 2009; 10(3):265-70. PMC: 3021958. DOI: 10.1002/mabi.200900363. View

Milleret V, Simona B, Neuenschwander P, Hall H . Tuning electrospinning parameters for production of 3D-fiber-fleeces with increased porosity for soft tissue engineering applications. Eur Cell Mater. 2011; 21:286-303. DOI: 10.22203/ecm.v021a22. View

10.

Vaquette C, Cooper-White J . Increasing electrospun scaffold pore size with tailored collectors for improved cell penetration. Acta Biomater. 2011; 7(6):2544-57. DOI: 10.1016/j.actbio.2011.02.036. View

11.

Han D, Gouma P . Electrospun bioscaffolds that mimic the topology of extracellular matrix. Nanomedicine. 2007; 2(1):37-41. DOI: 10.1016/j.nano.2006.01.002. View

12.

Chen X, Fu X, Shi J, Wang H . Regulation of the osteogenesis of pre-osteoblasts by spatial arrangement of electrospun nanofibers in two- and three-dimensional environments. Nanomedicine. 2013; 9(8):1283-92. DOI: 10.1016/j.nano.2013.04.013. View

13.

Guimaraes A, Martins A, Pinho E, Faria S, Reis R, Neves N . Solving cell infiltration limitations of electrospun nanofiber meshes for tissue engineering applications. Nanomedicine (Lond). 2010; 5(4):539-54. DOI: 10.2217/nnm.10.31. View

14.

Lee J, Jeong S, Bae M, Yang D, Heo D, Kim C . Highly porous electrospun nanofibers enhanced by ultrasonication for improved cellular infiltration. Tissue Eng Part A. 2011; 17(21-22):2695-702. DOI: 10.1089/ten.TEA.2010.0709. View

15.

Ki C, Park S, Kim H, Jung H, Woo K, Lee J . Development of 3-D nanofibrous fibroin scaffold with high porosity by electrospinning: implications for bone regeneration. Biotechnol Lett. 2007; 30(3):405-10. DOI: 10.1007/s10529-007-9581-5. View

16.

Baker B, Gee A, Metter R, Nathan A, Marklein R, Burdick J . The potential to improve cell infiltration in composite fiber-aligned electrospun scaffolds by the selective removal of sacrificial fibers. Biomaterials. 2008; 29(15):2348-58. PMC: 2292637. DOI: 10.1016/j.biomaterials.2008.01.032. View