Detection of Heat-stable Enterotoxigenic Escherichia Coli by Hybridization with an RNA Transcript Probe

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1987 Aug 1

PMID 2442192

Citations 2

Authors

O Chityothin

O Sethabutr

P Echeverria

D N Taylor

U Vongsthongsri

S Tharavanij

Affiliations

Soon will be listed here.

Abstract

Heat-stable enterotoxigenic Escherichia coli was identified by nucleotide hybridization with RNA transcripts of the gene encoding heat-stable A-2 enterotoxin. Radiolabeled enterotoxin gene RNA transcripts are easier to prepare and avoid the preparation of cloned DNA probes that can be nonspecific if they contain cloning vector DNA.

Citing Articles

Preparation of a specific RNA probe for detection of Mycobacterium paratuberculosis and diagnosis of Johne's disease.

Poupart P, Coene M, Van Heuverswyn H, Cocito C J Clin Microbiol. 1993; 31(6):1601-5.

PMID: 8315002 PMC: 265585. DOI: 10.1128/jcm.31.6.1601-1605.1993.

Simultaneous detection of Escherichia coli heat-stable and heat-labile enterotoxin genes with a single RNA probe.

Saez-Llorens X, Shelton S, Nelson J, Kupersztoch Y J Clin Microbiol. 1989; 27(7):1684-8.

PMID: 2671025 PMC: 267644. DOI: 10.1128/jcm.27.7.1684-1688.1989.

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