Article: Studies on thermoluminescence, delayed light emission and oxygen evolution from photosynthetic materials: UV effects

The effect of ultraviolet light on thermoluminescence, oxygen evolution and the slow component of delayed light has been investigated in chloroplasts and Pothos leaves. All peaks including peak V (48°C) were inhibited by UV. However, the peak at 48°C which was induced by DCMU was enhanced following UV irradiation of chloroplasts at ambient temperature (23°C) whereas peak II (-12°C) and peak III (10°C) which were also induced by DCMU were inhibited. Chloroplasts treated with DCMU and dark incubated for several minutes at ambient temperature prior to recording of glow curves have also shown enhancement of peak at 48°C. A slow component of delayed light and photosystem II activity of chloroplasts were inhibited by UV whereas photosystem I activity was marginally affected. These results corroborate involvement of photosystem II in generating thermoluminescence and slow components of delayed light in photosynthetic materials.

Citing Articles

Inactivation of photosynthetic oxygen evolution by UV-B irradiation: A thermoluminescence study.

Hideg E, Sass L, Barbato R, Vass I Photosynth Res. 2013; 38(3):455-62.

PMID: 24318003 DOI: 10.1007/BF00046774.

Thermoluminescence from the photosynthetic apparatus.

Vass I, Govindjee Photosynth Res. 2013; 48(1-2):117-26.

PMID: 24271292 DOI: 10.1007/BF00041002.

The history of photosynthetic thermoluminescence.

Vass I Photosynth Res. 2005; 76(1-3):303-18.

PMID: 16228589 DOI: 10.1023/A:1024989519054.

References

1.

Sane P, Goodchild D, Park R . Characterization of chloroplast photosystems 1 and 2 separated by a non-detergent method. Biochim Biophys Acta. 1970; 216(1):162-78. DOI: 10.1016/0005-2728(70)90168-4. View

2.

Greer D, Berry J, Bjorkman O . Photoinhibition of photosynthesis in intact bean leaves: role of light and temperature, and requirement for chloroplast-protein synthesis during recovery. Planta. 2013; 168(2):253-60. DOI: 10.1007/BF00402971. View

3.

Jones L, Kok B . Photoinhibition of Chloroplast Reactions. II. Multiple Effects. Plant Physiol. 1966; 41(6):1044-9. PMC: 1086470. DOI: 10.1104/pp.41.6.1044. View

4.

Inoue Y, Shibata K . Oscillation of thermoluminescence at medium-low temperature. FEBS Lett. 1978; 85(2):193-7. DOI: 10.1016/0014-5793(78)80453-0. View

5.

Arnold W, Azzi J . Chlorophyll energy levels and electron flow in photosynthesis. Proc Natl Acad Sci U S A. 1968; 61(1):29-35. PMC: 285900. DOI: 10.1073/pnas.61.1.29. View

6.

Rutherford A, Govindjee , Inoue Y . Charge accumulation and photochemistry in leaves studied by thermoluminescence and delayed light emission. Proc Natl Acad Sci U S A. 1984; 81(4):1107-11. PMC: 344774. DOI: 10.1073/pnas.81.4.1107. View

7.

Gregory R, Droppa M, Horvath G, Evans E . A comparison based on delayed light emission and fluorescence induction of intact chloroplasts isolated from mesophyll protoplasts and bundle-sheath cells of maize. Biochem J. 1979; 180(1):253-6. PMC: 1161043. DOI: 10.1042/bj1800253. View

8.

Desai T, Sane P, TATAKE V . Thermoluminescence studies on spinach leaves and Euglena. Photochem Photobiol. 1975; 21(5):345-50. DOI: 10.1111/j.1751-1097.1975.tb06682.x. View

9.

Kyle D, Ohad I, Arntzen C . Membrane protein damage and repair: Selective loss of a quinone-protein function in chloroplast membranes. Proc Natl Acad Sci U S A. 1984; 81(13):4070-4. PMC: 345370. DOI: 10.1073/pnas.81.13.4070. View

10.

Ichikawa T, Inoue Y, Shibata K . Characteristics of thermoluminescence bands of intact leaves and isolated chloroplasts in relation to the water-splitting activity in photosynthesis. Biochim Biophys Acta. 1975; 408(3):228-39. DOI: 10.1016/0005-2728(75)90126-7. View

11.

Inoue Y, Yamashita T, Kobayashi Y, Shibata K . Thermoluminescence changes during inactivation and reactivation of the oxygen-evolving system in isolated chloroplasts. FEBS Lett. 1977; 82(2):303-6. DOI: 10.1016/0014-5793(77)80607-8. View

Studies on Thermoluminescence, Delayed Light Emission and Oxygen Evolution from Photosynthetic Materials: UV Effects