Integrin α1β1 Differentially Regulates Cytokine-mediated Responses in Chondrocytes

Overview

Journal Osteoarthritis Cartilage

Specialties Orthopedics
Rheumatology

Date 2014 Jan 15

PMID 24418674

Citations 8

Authors

R Parekh

M K Lorenzo

S Y Shin

A Pozzi

A L Clark

Affiliations

Soon will be listed here.

Abstract

Objective: To elucidate the role of integrin α1β1 in chondrocyte responses to inflammatory interleukin-1α (IL-1) and anabolic transforming growth factor-β1 (TGF-β1) in the knee.

Methods: Intracellular calcium transient responses to IL-1 and TGF-β1 were measured in wild type and integrin α1-null chondrocytes using real time ex vivo confocal microscopy, and immunohistochemistry was performed to analyze TGF-β1-mediated activation of Smad2/3 in tibial and femoral chondrocytes.

Results: Loss of integrin α1β1 reduces intracellular calcium transient response to IL-1, while it enhances chondrocyte responses to TGF-β1 as measured by intracellular calcium transients and activation of downstream Smad2/3.

Conclusions: Integrin α1β1 plays a vital role in mediating chondrocyte responses to two contrasting factors that are critical players in the onset and progression of osteoarthritis - inflammatory IL-1 and anabolic TGF-β. Further investigation into the molecular mechanisms by which integrin α1β1 mediates these responses will be an important next step in understanding the influence of increased expression of integrin α1β1 during the early stages of osteoarthritis on disease progression.

Citing Articles

Depleting transforming growth factor beta receptor 2 signalling in the cartilage of -null mice attenuates spontaneous knee osteoarthritis.

Amant J, Michaud J, Hinds D, Coyle M, Pozzi A, Clark A Osteoarthr Cartil Open. 2023; 5(4):100399.

PMID: 37649532 PMC: 10462827. DOI: 10.1016/j.ocarto.2023.100399.

Chondrocyte primary cilia lengthening and shortening in response to mediators of osteoarthritis; a role for integrin α1β1 and focal adhesions.

Stam L, Clark A Osteoarthr Cartil Open. 2023; 5(2):100357.

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Atomic Force Microscopy-Based Nanoscopy of Chondrogenically Differentiating Human Adipose-Derived Stem Cells: Nanostructure and Integrin β1 Expression.

Yang J, He M, Huang X, Wang Q, Pi J, Wang H Nanoscale Res Lett. 2018; 13(1):333.

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Quisenberry C, Nazempour A, Van Wie B, Abu-Lail N Biointerphases. 2016; 11(2):021005.

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