Implementation of a Novel in Vitro Model of Infection of Reconstituted Human Epithelium for Expression of Virulence Genes in Methicillin-resistant Staphylococcus Aureus Strains Isolated from Catheter-related Infections in Mexico

Overview

Journal Ann Clin Microbiol Antimicrob

Publisher Biomed Central

Specialties Infectious Diseases
Microbiology

Date 2014 Jan 11

PMID 24405688

Citations 3

Authors

Gloria Luz Paniagua-Contreras

Eric Monroy-Perez

Felipe Vaca-Paniagua

Jose Raymundo Rodriguez-Moctezuma

Erasmo Negrete-Abascal

Sergio Vaca

Affiliations

Soon will be listed here.

Abstract

Background: Methicillin-resistant Staphylococcus aureus (MRSA) are clinically relevant pathogens that cause severe catheter-related nosocomial infections driven by several virulence factors.

Methods: We implemented a novel model of infection in vitro of reconstituted human epithelium (RHE) to analyze the expression patterns of virulence genes in 21 MRSA strains isolated from catheter-related infections in Mexican patients undergoing haemodialysis. We also determined the phenotypic and genotypic co-occurrence of antibiotic- and disinfectant-resistance traits in the S. aureus strains, which were also analysed by pulsed-field-gel electrophoresis (PFGE).

Results: In this study, MRSA strains isolated from haemodialysis catheter-related infections expressed virulence markers that mediate adhesion to, and invasion of, RHE. The most frequent pattern of expression (present in 47.6% of the strains) was as follows: fnbA, fnbB, spa, clfA, clfB, cna, bbp, ebps, eap, sdrC, sdrD, sdrE, efb, icaA, and agr. Seventy-one percent of the strains harboured the antibiotic- and disinfectant-resistance genes ermA, ermB, tet(M), tet(K), blaZ, qacA, qacB, and qacC. PFGE of the isolated MRSA revealed three identical strains and two pairs of identical strains. The strains with identical PFGE patterns showed the same phenotypes and genotypes, including the same spa type (t895), suggesting hospital personnel manipulating the haemodialysis equipment could be the source of catheter contamination.

Conclusion: These findings help define the prevalence of MRSA virulence factors in catheter-related infections. Some of the products of the expressed genes that we detected in this work may serve as potential antigens for inclusion in a vaccine for the prevention of MRSA-catheter-related infections.

Citing Articles

High prevalence of t895 and t9364 spa types of methicillin-resistant Staphylococcus aureus in a tertiary-care hospital in Mexico: different lineages of clonal complex 5.

Negrete-Gonzalez C, Turrubiartes-Martinez E, Galicia-Cruz O, Noyola D, Martinez-Aguilar G, Perez-Gonzalez L BMC Microbiol. 2020; 20(1):213.

PMID: 32689948 PMC: 7370520. DOI: 10.1186/s12866-020-01881-w.

Flipping the switch: tools for detecting small molecule inhibitors of staphylococcal virulence.

Quave C, Horswill A Front Microbiol. 2015; 5:706.

PMID: 25566220 PMC: 4264471. DOI: 10.3389/fmicb.2014.00706.

Expression of enterotoxin-coding genes in methicillin-resistant Staphylococcus aureus strains isolated from Mexican haemodialysis patients.

Paniagua-Contreras G, Monroy-Perez E, Vaca-Paniagua F, Rodriguez-Moctezuma J, Vaca S Ann Clin Microbiol Antimicrob. 2014; 13:55.

PMID: 25421262 PMC: 4245768. DOI: 10.1186/s12941-014-0055-z.

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