A Negative-pressure-driven Microfluidic Chip for the Rapid Detection of a Bladder Cancer Biomarker in Urine Using Bead-based Enzyme-linked Immunosorbent Assay

Overview

Journal Biomicrofluidics

Publisher American Institute of Physics

Specialty Biomedical Engineering

Date 2014 Jan 10

PMID 24404008

Citations 16

Authors

Yen-Heng Lin

Ying-Ju Chen

Chao-Sung Lai

Yi-Ting Chen

Chien-Lun Chen

Jau-Song Yu

Yu-Sun Chang

Affiliations

Soon will be listed here.

Abstract

This paper describes an integrated microfluidic chip that is capable of rapidly and quantitatively measuring the concentration of a bladder cancer biomarker, apolipoprotein A1, in urine samples. All of the microfluidic components, including the fluid transport system, the micro-valve, and the micro-mixer, were driven by negative pressure, which simplifies the use of the chip and facilitates commercialization. Magnetic beads were used as a solid support for the primary antibody, which captured apolipoprotein A1 in patients' urine. Because of the three-dimensional structure of the magnetic beads, the concentration range of the target that could be detected was as high as 2000 ng ml(-1). Because this concentration is 100 times higher than that quantifiable using a 96-well plate with the same enzyme-linked immunosorbent assay (ELISA) kit, the dilution of the patient's urine can be avoided or greatly reduced. The limit of detection was determined to be approximately 10 ng ml(-1), which is lower than the cutoff value for diagnosing bladder cancer (11.16 ng ml(-1)). When the values measured using the microfluidic chip were compared with those measured using conventional ELISA using a 96-well plate for five patients, the deviations were 0.9%, 6.8%, 9.4%, 1.8%, and 5.8%. The entire measurement time is 6-fold faster than that of conventional ELISA. This microfluidic device shows significant potential for point-of-care applications.

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