Noninvasive Detection of Lung Cancer Using Exhaled Breath

Overview

Journal Cancer Med

Specialty Oncology

Date 2014 Jan 10

PMID 24402867

Citations 48

Authors

Xiao-An Fu

Mingxiao Li

Ralph J Knipp

Michael H Nantz

Michael Bousamra

Affiliations

Soon will be listed here.

Abstract

Early detection of lung cancer is a key factor for increasing the survival rates of lung cancer patients. The analysis of exhaled breath is promising as a noninvasive diagnostic tool for diagnosis of lung cancer. We demonstrate the quantitative analysis of carbonyl volatile organic compounds (VOCs) and identification of lung cancer VOC markers in exhaled breath using unique silicon microreactor technology. The microreactor consists of thousands of micropillars coated with an ammonium aminooxy salt for capture of carbonyl VOCs in exhaled breath by means of oximation reactions. Captured aminooxy-VOC adducts are analyzed by nanoelectrospray Fourier transform-ion cyclotron resonance (FT-ICR) mass spectrometry (MS). The concentrations of 2-butanone, 2-hydroxyacetaldehyde, 3-hydroxy-2-butanone, and 4-hydroxyhexenal (4-HHE) in the exhaled breath of lung cancer patients (n = 97) were significantly higher than in the exhaled breath of healthy smoker and nonsmoker controls (n = 88) and patients with benign pulmonary nodules (n = 32). The concentration of 2-butanone in exhaled breath of patients (n = 51) with stages II though IV non-small cell lung cancer (NSCLC) was significantly higher than in exhaled breath of patients with stage I (n = 34). The carbonyl VOC profile in exhaled breath determined using this new silicon microreactor technology provides for the noninvasive detection of lung cancer.

Citing Articles

Differentiating Pulmonary Nodule Malignancy Using Exhaled Volatile Organic Compounds: A Prospective Observational Study.

Lu G, Su Z, Yu X, He Y, Sha T, Yan K Cancer Med. 2025; 14(1):e70545.

PMID: 39777868 PMC: 11706237. DOI: 10.1002/cam4.70545.

Collection methods of exhaled volatile organic compounds for lung cancer screening and diagnosis: a systematic review.

He Y, Su Z, Sha T, Yu X, Guo H, Tao Y J Thorac Dis. 2024; 16(11):7978-7998.

PMID: 39678905 PMC: 11635225. DOI: 10.21037/jtd-24-1001.

Inconsistencies in predictive models based on exhaled volatile organic compounds for distinguishing between benign pulmonary nodules and lung cancer: a systematic review.

Su Z, Yu X, He Y, Sha T, Guo H, Tao Y BMC Pulm Med. 2024; 24(1):551.

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Current Evidence for a Lung Cancer Screening Program.

Guerreiro T, Aguiar P, Araujo A Port J Public Health. 2024; 42(2):133-158.

PMID: 39469231 PMC: 11498919. DOI: 10.1159/000538434.

Diagnosis of Lung Cancer Through Exhaled Breath: A Comprehensive Study.

Gashimova E, Temerdashev A, Perunov D, Porkhanov V, Polyakov I Mol Diagn Ther. 2024; 28(6):847-860.

PMID: 39299985 DOI: 10.1007/s40291-024-00744-8.

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