Mismatches Improve the Performance of Strand-displacement Nucleic Acid Circuits

Overview

Journal Angew Chem Int Ed Engl

Specialty Chemistry

Date 2014 Jan 10

PMID 24402831

Citations 32

Authors

Yu Sherry Jiang

Sanchita Bhadra

Bingling Li

Andrew D Ellington

Affiliations

Soon will be listed here.

Abstract

Catalytic hairpin assembly (CHA) has previously proven useful as a transduction and amplification method for nucleic acid detection. However, the two hairpin substrates in a CHA circuit can potentially react non-specifically even in the absence of a single-stranded catalyst, and this non-specific background degrades the signal-to-noise ratio. The introduction of mismatched base pairs that impede uncatalyzed strand exchange reactions led to a significant decrease of the background signal, while only partially damping the signal in the presence of a catalyst. Various types and lengths of mismatches were assayed by fluorimetry, and in many instances, our MismatCHA designs yielded 100-fold increased signal-to-background ratios compared to a ratio of 4:1 with the perfectly matched substrates. These observations could be of general utility for the design of non-enzymatic nucleic acid circuits.

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