Rapid Ertapenem Susceptibility Testing and Klebsiella Pneumoniae Carbapenemase Phenotype Detection in Klebsiella Pneumoniae Isolates by Use of Automated Microscopy of Immobilized Live Bacterial Cells

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2014 Jan 7

PMID 24391202

Citations 12

Authors

Carey-Ann D Burnham

Rachel A Frobel

Monica L Herrera

Brian L Wickes

Affiliations

Soon will be listed here.

Abstract

We evaluated detection of ertapenem (ETP) resistance and Klebsiella pneumoniae carbapenemase (KPC) in 47 Klebsiella pneumoniae isolates using a novel automated microscopy system. Automated microscopy correctly classified 22/23 isolates as ETP resistant and 24/24 as ETP susceptible and correctly classified 21/21 isolates as KPC positive and 26/26 as KPC negative.

Citing Articles

Laboratory detection of carbapenemases among Gram-negative organisms.

Simner P, Pitout J, Dingle T Clin Microbiol Rev. 2024; 37(4):e0005422.

PMID: 39545731 PMC: 11629623. DOI: 10.1128/cmr.00054-22.

Next-generation rapid phenotypic antimicrobial susceptibility testing.

Reszetnik G, Hammond K, Mahshid S, AbdElFatah T, Nguyen D, Corsini R Nat Commun. 2024; 15(1):9719.

PMID: 39521792 PMC: 11550857. DOI: 10.1038/s41467-024-53930-x.

Rapid detection of carbapenem resistance among gram-negative organisms directly from positive blood culture bottles.

Kumar M, Tandel K, Shergill S, Bhalla G, Mahajan P, Swarnim V Med J Armed Forces India. 2023; 79(3):267-274.

PMID: 37193528 PMC: 10182285. DOI: 10.1016/j.mjafi.2021.03.026.

Differential DNA accessibility to polymerase enables 30-minute phenotypic β-lactam antibiotic susceptibility testing of carbapenem-resistant Enterobacteriaceae.

Schoepp N, Liaw E, Winnett A, Savela E, Garner O, Ismagilov R PLoS Biol. 2020; 18(3):e3000652.

PMID: 32191697 PMC: 7081982. DOI: 10.1371/journal.pbio.3000652.

Emerging strategies for the noninvasive diagnosis of nosocomial pneumonia.

Liapikou A, Cilloniz C, Torres A Expert Rev Anti Infect Ther. 2019; 17(7):523-533.

PMID: 31237462 PMC: 7103721. DOI: 10.1080/14787210.2019.1635010.

References

Munoz-Price L, Poirel L, Bonomo R, Schwaber M, Daikos G, Cormican M . Clinical epidemiology of the global expansion of Klebsiella pneumoniae carbapenemases. Lancet Infect Dis. 2013; 13(9):785-96. PMC: 4673667. DOI: 10.1016/S1473-3099(13)70190-7. View

Ke W, Bethel C, Papp-Wallace K, Pagadala S, Nottingham M, Fernandez D . Crystal structures of KPC-2 β-lactamase in complex with 3-nitrophenyl boronic acid and the penam sulfone PSR-3-226. Antimicrob Agents Chemother. 2012; 56(5):2713-8. PMC: 3346646. DOI: 10.1128/AAC.06099-11. View

Patel G, Bonomo R . "Stormy waters ahead": global emergence of carbapenemases. Front Microbiol. 2013; 4:48. PMC: 3596785. DOI: 10.3389/fmicb.2013.00048. View

Drew R, Turton J, Hill R, Livermore D, Woodford N, Paulus S . Emergence of carbapenem-resistant Enterobacteriaceae in a UK paediatric hospital. J Hosp Infect. 2013; 84(4):300-4. DOI: 10.1016/j.jhin.2013.05.003. View

Doern C, Dunne Jr W, Burnham C . Detection of Klebsiella pneumoniae carbapenemase (KPC) production in non-Klebsiella pneumoniae Enterobacteriaceae isolates by use of the Phoenix, Vitek 2, and disk diffusion methods. J Clin Microbiol. 2011; 49(3):1143-7. PMC: 3067756. DOI: 10.1128/JCM.02163-10. View

Mahenthiralingam E, CAMPBELL M, Foster J, Lam J, Speert D . Random amplified polymorphic DNA typing of Pseudomonas aeruginosa isolates recovered from patients with cystic fibrosis. J Clin Microbiol. 1996; 34(5):1129-35. PMC: 228968. DOI: 10.1128/jcm.34.5.1129-1135.1996. View

Rasheed J, Biddle J, Anderson K, Washer L, Chenoweth C, Perrin J . Detection of the Klebsiella pneumoniae carbapenemase type 2 Carbapenem-hydrolyzing enzyme in clinical isolates of Citrobacter freundii and K. oxytoca carrying a common plasmid. J Clin Microbiol. 2008; 46(6):2066-9. PMC: 2446874. DOI: 10.1128/JCM.02038-07. View

Maslow J, Mulligan M, Arbeit R . Molecular epidemiology: application of contemporary techniques to the typing of microorganisms. Clin Infect Dis. 1993; 17(2):153-62; quiz 163-4. DOI: 10.1093/clinids/17.2.153. View

Tenover F, Kalsi R, Williams P, Carey R, Stocker S, Lonsway D . Carbapenem resistance in Klebsiella pneumoniae not detected by automated susceptibility testing. Emerg Infect Dis. 2006; 12(8):1209-13. PMC: 3291231. DOI: 10.3201/eid1208.060291. View

10.

Chiu S, Wu T, Chuang Y, Lin J, Fung C, Lu P . National surveillance study on carbapenem non-susceptible Klebsiella pneumoniae in Taiwan: the emergence and rapid dissemination of KPC-2 carbapenemase. PLoS One. 2013; 8(7):e69428. PMC: 3722148. DOI: 10.1371/journal.pone.0069428. View

11.

Bulik C, Fauntleroy K, Jenkins S, Abuali M, LaBombardi V, Nicolau D . Comparison of meropenem MICs and susceptibilities for carbapenemase-producing Klebsiella pneumoniae isolates by various testing methods. J Clin Microbiol. 2010; 48(7):2402-6. PMC: 2897473. DOI: 10.1128/JCM.00267-10. View

12.

Liu P, Lau Y, Hu B, Shyr J, Shi Z, Tsai W . Analysis of clonal relationships among isolates of Shigella sonnei by different molecular typing methods. J Clin Microbiol. 1995; 33(7):1779-83. PMC: 228268. DOI: 10.1128/jcm.33.7.1779-1783.1995. View

13.

Kruse E, Aurbach U, Wisplinghoff H . Carbapenem-Resistant Enterobacteriaceae: Laboratory Detection and Infection Control Practices. Curr Infect Dis Rep. 2013; . DOI: 10.1007/s11908-013-0373-x. View

14.

Casarez E, Pillai S, Di Giovanni G . Genotype diversity of Escherichia coli isolates in natural waters determined by PFGE and ERIC-PCR. Water Res. 2007; 41(16):3643-8. DOI: 10.1016/j.watres.2007.03.020. View

15.

Tzouvelekis L, Markogiannakis A, Psichogiou M, Tassios P, Daikos G . Carbapenemases in Klebsiella pneumoniae and other Enterobacteriaceae: an evolving crisis of global dimensions. Clin Microbiol Rev. 2012; 25(4):682-707. PMC: 3485753. DOI: 10.1128/CMR.05035-11. View

16.

Woodford N, Eastaway A, Ford M, Leanord A, Keane C, Quayle R . Comparison of BD Phoenix, Vitek 2, and MicroScan automated systems for detection and inference of mechanisms responsible for carbapenem resistance in Enterobacteriaceae. J Clin Microbiol. 2010; 48(8):2999-3002. PMC: 2916632. DOI: 10.1128/JCM.00341-10. View

17.

Hirsch E, Tam V . Detection and treatment options for Klebsiella pneumoniae carbapenemases (KPCs): an emerging cause of multidrug-resistant infection. J Antimicrob Chemother. 2010; 65(6):1119-25. DOI: 10.1093/jac/dkq108. View