Selective Activation of Human Dendritic Cells by OM-85 Through a NF-kB and MAPK Dependent Pathway

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2014 Jan 4

PMID 24386121

Citations 29

Authors

Carmen Parola

Laura Salogni

Xenia Vaira

Sara Scutera

Paolo Somma

Valentina Salvi

Tiziana Musso

Giuseppe Tabbia

Marco Bardessono

Christian Pasquali

Alberto Mantovani

Silvano Sozzani

Daniela Bosisio

Affiliations

Soon will be listed here.

Abstract

OM-85 (Broncho-Vaxom®, Broncho-Munal®, Ommunal®, Paxoral®, Vaxoral®), a product made of the water soluble fractions of 21 inactivated bacterial strain patterns responsible for respiratory tract infections, is used for the prevention of recurrent upper respiratory tract infections and acute exacerbations in chronic obstructive pulmonary disease patients. OM-85 is able to potentiate both innate and adaptive immune responses. However, the molecular mechanisms responsible for OM-85 activation are still largely unknown. Purpose of this study was to investigate the impact of OM-85 stimulation on human dendritic cell functions. We show that OM-85 selectively induced NF-kB and MAPK activation in human DC with no detectable action on the interferon regulatory factor (IRF) pathway. As a consequence, chemokines (i.e. CXCL8, CXCL6, CCL3, CCL20, CCL22) and B-cell activating cytokines (i.e. IL-6, BAFF and IL-10) were strongly upregulated. OM-85 also synergized with the action of classical pro-inflammatory stimuli used at suboptimal concentrations. Peripheral blood mononuclear cells from patients with COPD, a pathological condition often associated with altered PRR expression pattern, fully retained the capability to respond to OM-85. These results provide new insights on the molecular mechanisms of OM-85 activation of the immune response and strengthen the rational for its use in clinical settings.

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