Generation of Leukemia Inhibitory Factor-dependent Induced Pluripotent Stem Cells from the Massachusetts General Hospital Miniature Pig

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2013 Dec 28

PMID 24371815

Citations 17

Authors

Dae-Jin Kwon

Hyelena Jeon

Keon Bong Oh

Sun-A Ock

Gi-Sun Im

Sung-Soo Lee

Seok Ki Im

Jeong-Woong Lee

Sung-Jong Oh

Jin-Ki Park

Seongsoo Hwang

Affiliations

Soon will be listed here.

Abstract

The generation and application of porcine induced pluripotent stem cells (iPSCs) may enable the testing for safety and efficacy of therapy in the field of human regenerative medicine. Here, the generation of iPSCs from the Massachusetts General Hospital miniature pig (MGH minipig) established for organ transplantation studies is reported. Fibroblasts were isolated from the skin of the ear of a 10-day-old MGH minipig and transduced with a cocktail of six human factors: POU5F1, NANOG, SOX2, C-MYC, KLF4, and LIN28. Two distinct types of iPSCs were generated that were positive for alkaline phosphatase activity, as well as the classical pluripotency markers: Oct4, Nanog, Sox2, and the surface marker Ssea-1. Only one of two porcine iPSC lines differentiated into three germ layers both in vitro and in vivo. Western blot analysis showed that the porcine iPSCs were dependent on LIF or BMP-4 to sustain self-renewal and pluripotency. In conclusion, the results showed that human pluripotent factors could reprogram porcine ear fibroblasts into the pluripotent state. These cells may provide a useful source of cells that could be used for the treatment of degenerative and genetic diseases and agricultural research and application.

Citing Articles

The progress of induced pluripotent stem cells derived from pigs: a mini review of recent advances.

Neira J, Conrad J, Rusteika M, Chu L Front Cell Dev Biol. 2024; 12:1371240.

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Species-Specific Enhancer Activity of OCT4 in Porcine Pluripotency: The Porcine Reporter System Could Monitor Pluripotency in Porcine Embryo Development and Embryonic Stem Cells.

Kim S, Lee M, Choi K, Jeong J, Lee D, Oh J Stem Cells Int. 2022; 2022:6337532.

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Exogenous LIN28 Is Required for the Maintenance of Self-Renewal and Pluripotency in Presumptive Porcine-Induced Pluripotent Stem Cells.

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The use of induced pluripotent stem cells in domestic animals: a narrative review.

Scarfone R, Pena S, Russell K, Betts D, Koch T BMC Vet Res. 2020; 16(1):477.

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