Single Channel Kinetics of a Glutamate Receptor

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 1987 Jan 1

PMID 2436676

Citations 20

Authors

C J Kerry

K S Kits

R L Ramsey

M S Sansom

P N Usherwood

Affiliations

Soon will be listed here.

Abstract

The glutamate receptor-channel of locust muscle membrane was studied using the patch-clamp technique. Muscles were pretreated with concanavalin A to block receptor-channel desensitization, thus facilitating analysis of receptor-channel gating kinetics. Single channel kinetics were analyzed to aid in identification of the molecular basis of channel gating. Channel dwell-time distributions and dwell-time autocorrelation functions were calculated from single channel data recorded in the precence of 10-4M glutamate. Analysis of the dwell time distributions in terms of mixtures of exponential functions revealed there to be at least three open states of the receptor-channel and at least four closed states. Autocorrelation function analysis showed there to be at least three pathways linking the open states with the closed. This results in a minimal scheme for gating of the glutamate receptor-channel, which is suggestive of allosteric models of receptor-channel gating.

Citing Articles

Glutamate receptor channel kinetics: the effect of glutamate concentration.

Kerry C, Ramsey R, Sansom M, Usherwood P Biophys J. 2009; 53(1):39-52.

PMID: 19431716 PMC: 1330120. DOI: 10.1016/S0006-3495(88)83064-9.

The action of philanthotoxin-343 and photolabile analogues on locust (Schistocerca gregaria) muscle.

Sudan H, Kerry C, Mellor I, Choi S, Huang D, Nakanishi K Invert Neurosci. 1995; 1(2):159-72.

PMID: 9372140 DOI: 10.1007/BF02331913.

Gating kinetics of the quisqualate-sensitive glutamate receptor of locust muscle studied using agonist concentration jumps and computer simulations.

Standley C, Norris T, Ramsey R, Usherwood P Biophys J. 1993; 65(4):1379-86.

PMID: 8274632 PMC: 1225864. DOI: 10.1016/S0006-3495(93)81192-5.

Gating of the native and purified cardiac SR Ca(2+)-release channel with monovalent cations as permeant species.

Sitsapesan R, Williams A Biophys J. 1994; 67(4):1484-94.

PMID: 7819484 PMC: 1225511. DOI: 10.1016/S0006-3495(94)80622-8.

Single ion channel models incorporating aggregation and time interval omission.

Ball F, Yeo G, Milne R, Edeson R, Madsen B, Sansom M Biophys J. 1993; 64(2):357-74.

PMID: 7681333 PMC: 1262339. DOI: 10.1016/S0006-3495(93)81375-4.

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