Identification of Two Telomere-proximal Fission Yeast DNA Replication Origins Constrained by Nearby Cis-acting Sequences to Replicate in Late S Phase

Overview

Journal F1000Res

Specialties Biomedical Engineering
Science

Date 2013 Dec 24

PMID 24358832

Citations 3

Authors

Amna Chaudari

Joel A Huberman

Affiliations

Soon will be listed here.

Abstract

Telomeres of the fission yeast, Schizosaccharomyces pombe, are known to replicate in late S phase, but the reasons for this late replication are not fully understood. We have identified two closely-spaced DNA replication origins, 5.5 to 8 kb upstream from the telomere itself. These are the most telomere-proximal of all the replication origins in the fission yeast genome. When located by themselves in circular plasmids, these origins fired in early S phase, but if flanking sequences closer to the telomere were included in the circular plasmid, then replication was restrained to late S phase - except in cells lacking the replication-checkpoint kinase, Cds1. We conclude that checkpoint-dependent late replication of telomere-associated sequences is dependent on nearby cis-acting sequences, not on proximity to the physical end of a linear chromosome.

Citing Articles

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Yadav R, Matsuda A, Lowe B, Hiraoka Y, Partridge J Microorganisms. 2021; 9(9).

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Complete sequences of Schizosaccharomyces pombe subtelomeres reveal multiple patterns of genome variation.

Oizumi Y, Kaji T, Tashiro S, Takeshita Y, Date Y, Kanoh J Nat Commun. 2021; 12(1):611.

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TASks for subtelomeres: when nucleosome loss and genome instability are favored.

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