Zinc Finger X-chromosomal Protein Promotes Growth and Tumorigenesis in Human Osteosarcoma Cells

Overview

Journal Pak J Med Sci

Specialty General Medicine

Date 2013 Dec 20

PMID 24353675

Citations 3

Authors

Rui Jiang

Zhong-Li Gao

Mei Sun

Xing-Yi Zhang

Jin-Cheng Wang

Han Wu

Affiliations

Soon will be listed here.

Abstract

Objective: To investigate the role of Zinc finger X-chromosomal protein (ZFX) in oncogenesis of Osteosarcoma tumor.

Methods: Here, we first conducted an expression analysis of ZFX in Osteosarcoma cell lines. Then, we constructed ZFX-specific small interfering RNA (siRNA)-lentiviral vector that is capable of effectively inhibiting the expression of ZFX gene in human Osteosarcoma Saos-2 cells, and investigated systemically the impacts of ZFX silence on the growth and invasive ability of the cancer cells in vitro. Furthermore, we determined the effects of ZFX knockdown on the cell cycle distribution and apoptosis of Saos-2 cells.

Results: We found that ZFX inhibition resulted in significantly impaired proliferation and colony formation as well as mitigated invasiveness of Saos-2 cells. Importantly, si-ZFX infected cells exhibited a greater portion of cells at G1 phase, but a minor portion of S and G2/M phase cells. Moreover, a greater portion of sub-G1 apoptotic cells was observed in si-ZFX infected cells.

Conclusions: These results strongly suggest that ZFX is a novel proliferation regulator that promotes growth of Osteosarcoma cells, and downregulation of ZFX expression induces growth suppression of Saos-2 cells via arrested G0/G1 phase cell cycle and apoptosis pathways, thereby indicating that ZFX may serve as a new molecular target for Osteosarcoma tumor therapy.

Citing Articles

Zinc finger protein X-linked is overexpressed in colorectal cancer and is associated with poor prognosis.

Jiang J, Liu L Oncol Lett. 2015; 10(2):810-814.

PMID: 26622575 PMC: 4509075. DOI: 10.3892/ol.2015.3353.

Expression of ZFX gene correlated with the central features of the neoplastic phenotype in human brain tumors with distinct phenotypes.

Afzali A, Emadi-Baygi M, Nikpour P, Nazemroaya F, Kheirollahi M Adv Biomed Res. 2015; 4:179.

PMID: 26605218 PMC: 4617010. DOI: 10.4103/2277-9175.164000.

Zinc finger X-chromosomal protein (ZFX) is a significant prognostic indicator and promotes cellular malignant potential in gallbladder cancer.

Weng H, Wang X, Li M, Wu X, Wang Z, Wu W Cancer Biol Ther. 2015; 16(10):1462-70.

PMID: 26230915 PMC: 4846125. DOI: 10.1080/15384047.2015.1070994.

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