Contrast-enhanced MicroCT (EPIC-μCT) Ex Vivo Applied to the Mouse and Human Jaw Joint

Overview

Journal Dentomaxillofac Radiol

Publisher Oxford University Press

Date 2013 Dec 20

PMID 24353248

Citations 8

Authors

G A P Renders

L Mulder

A S Lin

G E J Langenbach

J H Koolstra

R E Guldberg

V Everts

Affiliations

Soon will be listed here.

Abstract

Objectives: The temporomandibular joint (TMJ) is susceptive to the development of osteoarthritis (OA). More detailed knowledge of its development is essential to improve our insight into TMJ-OA. It is imperative to have a standardized reliable three-dimensional (3D) imaging method that allows for detailed assessment of both bone and cartilage in healthy and diseased joints. We aimed to determine the applicability of a contrast-enhanced microCT (µCT) technique for ex vivo research of mouse and human TMJs.

Methods: Equilibrium partitioning of an ionic contrast agent via µCT (EPIC-µCT) was previously applied for cartilage assessment in the knee joint. The method was ex vivo, applied to the mouse TMJ and adapted for the human TMJ.

Results: EPIC-µCT (30-min immersion time) was applied to mouse mandibular condyles, and 3D imaging revealed an average cartilage thickness of 110 ± 16 µm. These measurements via EPIC-µCT were similar to the histomorphometric measures (113 ± 19 µm). For human healthy OA-affected TMJ samples, the protocol was adjusted to an immersion time of 1 h. 3D imaging revealed a significant thicker cartilage layer in joints with early signs of OA compared with healthy joints (414.2 ± 122.6 and 239.7 ± 50.5 µm, respectively). A subsequent significant thinner layer was found in human joints with late signs of OA (197.4 ± 159.7 µm).

Conclusions: The EPIC-µCT technique is effective for the ex vivo assessment of 3D cartilage morphology in the mouse as well as human TMJ and allows bone-cartilage interaction research in TMJ-OA.

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References

Kameoka S, Kuroki Y, Honda K, Kijima N, Matsumoto K, Asano M . Diagnostic accuracy of microcomputed tomography for osseous abnormalities in the rat temporomandibular joint condyle. Dentomaxillofac Radiol. 2009; 38(7):465-9. DOI: 10.1259/dmfr/24350438. View

Renders G, Mulder L, van Ruijven L, van Eijden T . Degree and distribution of mineralization in the human mandibular condyle. Calcif Tissue Int. 2006; 79(3):190-6. DOI: 10.1007/s00223-006-0015-5. View

Joshi N, Bansal P, Stewart R, Snyder B, Grinstaff M . Effect of contrast agent charge on visualization of articular cartilage using computed tomography: exploiting electrostatic interactions for improved sensitivity. J Am Chem Soc. 2009; 131(37):13234-5. DOI: 10.1021/ja9053306. View

Zarb G, Carlsson G . Temporomandibular disorders: osteoarthritis. J Orofac Pain. 2000; 13(4):295-306. View

Siebelt M, van Tiel J, Waarsing J, Piscaer T, van Straten M, Booij R . Clinically applied CT arthrography to measure the sulphated glycosaminoglycan content of cartilage. Osteoarthritis Cartilage. 2011; 19(10):1183-9. DOI: 10.1016/j.joca.2011.07.006. View

Kotwal N, Li J, Sandy J, Plaas A, Sumner D . Initial application of EPIC-μCT to assess mouse articular cartilage morphology and composition: effects of aging and treadmill running. Osteoarthritis Cartilage. 2012; 20(8):887-95. PMC: 3817026. DOI: 10.1016/j.joca.2012.04.012. View

Palmer A, Guldberg R, Levenston M . Analysis of cartilage matrix fixed charge density and three-dimensional morphology via contrast-enhanced microcomputed tomography. Proc Natl Acad Sci U S A. 2006; 103(51):19255-60. PMC: 1748213. DOI: 10.1073/pnas.0606406103. View

Shen G, Darendeliler M . The adaptive remodeling of condylar cartilage---a transition from chondrogenesis to osteogenesis. J Dent Res. 2005; 84(8):691-9. DOI: 10.1177/154405910508400802. View

Chen J, Sorensen K, Gupta T, Kilts T, Young M, Wadhwa S . Altered functional loading causes differential effects in the subchondral bone and condylar cartilage in the temporomandibular joint from young mice. Osteoarthritis Cartilage. 2008; 17(3):354-61. PMC: 2646810. DOI: 10.1016/j.joca.2008.05.021. View

10.

Kurkijarvi J, Mattila L, Ojala R, Vasara A, Jurvelin J, Kiviranta I . Evaluation of cartilage repair in the distal femur after autologous chondrocyte transplantation using T2 relaxation time and dGEMRIC. Osteoarthritis Cartilage. 2006; 15(4):372-8. DOI: 10.1016/j.joca.2006.10.001. View

11.

Radin E, Rose R . Role of subchondral bone in the initiation and progression of cartilage damage. Clin Orthop Relat Res. 1986; (213):34-40. View

12.

Benders K, Malda J, Saris D, Dhert W, Steck R, Hutmacher D . Formalin fixation affects equilibrium partitioning of an ionic contrast agent-microcomputed tomography (EPIC-μCT) imaging of osteochondral samples. Osteoarthritis Cartilage. 2010; 18(12):1586-91. DOI: 10.1016/j.joca.2010.10.005. View

13.

Byers P, Contepomi C, Farkas T . A post mortem study of the hip joint. Including the prevalence of the features of the right side. Ann Rheum Dis. 1970; 29(1):15-31. PMC: 1031217. DOI: 10.1136/ard.29.1.15. View

14.

Hansson T, Oberg T, Carlsson G, Kopp S . Thickness of the soft tissue layers and the articular disk in the temporomandibular joint. Acta Odontol Scand. 1977; 35(2):77-83. DOI: 10.3109/00016357709055993. View

15.

Xie L, Lin A, Levenston M, Guldberg R . Quantitative assessment of articular cartilage morphology via EPIC-microCT. Osteoarthritis Cartilage. 2008; 17(3):313-20. PMC: 2683349. DOI: 10.1016/j.joca.2008.07.015. View

16.

Sano T, Otonari-Yamamoto M, Otonari T, Yajima A . Osseous abnormalities related to the temporomandibular joint. Semin Ultrasound CT MR. 2007; 28(3):213-21. DOI: 10.1053/j.sult.2007.02.006. View

17.

Liang G, VanHouten J, Macica C . An atypical degenerative osteoarthropathy in Hyp mice is characterized by a loss in the mineralized zone of articular cartilage. Calcif Tissue Int. 2011; 89(2):151-62. DOI: 10.1007/s00223-011-9502-4. View

18.

Jiao K, Niu L, Wang M, Dai J, Yu S, Liu X . Subchondral bone loss following orthodontically induced cartilage degradation in the mandibular condyles of rats. Bone. 2010; 48(2):362-71. DOI: 10.1016/j.bone.2010.09.010. View

19.

Widmalm S, Brooks S, Sano T, Upton L, McKay D . Limitation of the diagnostic value of MR images for diagnosing temporomandibular joint disorders. Dentomaxillofac Radiol. 2006; 35(5):334-8. DOI: 10.1259/dmfr/23427399. View

20.

Day J, van der Linden J, Bank R, Ding M, Hvid I, Sumner D . Adaptation of subchondral bone in osteoarthritis. Biorheology. 2004; 41(3-4):359-68. View