A Modified Dinucleotide for Site-specific RNA-labelling by Transcription Priming and Click Chemistry

Overview

Journal Chem Commun (Camb)

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2013 Dec 18

PMID 24343756

Citations 13

Authors

Ayan Samanta

Andre Krause

Andres Jaschke

Affiliations

Soon will be listed here.

Abstract

An improved strategy for RNA labelling using an alkyne-carrying dinucleotide is reported. This involves near-quantitative priming by phage RNA-polymerases followed by conjugation of different labels using click chemistry. Moreover, these transcripts bear a ligation compatible 5'-end, and thus through ligation the terminal label can be transformed to an internal one.

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