Genotoxicity of Indium Tin Oxide by Allium and Comet Tests

Overview

Journal Cytotechnology

Specialties Biotechnology
Genetics

Date 2013 Dec 17

PMID 24337653

Citations 9

Authors

Ibrahim Hakki Cigerci

Recep Liman

Emre Ozgul

Muhsin Konuk

Affiliations

Soon will be listed here.

Abstract

Genotoxic effects of indium tin oxide (ITO) were investigated on root cells of Allium cepa by employing both Allium and Comet assays. A. cepa roots were treated with the aqueous dispersions of ITO at 5 different concentrations (12.5, 25, 50, 75, and 100 ppm) for 4 h. Exposure of ITO significantly increased mitotic index, and total chromosomal aberrations by the Allium test. While chromosome laggards, stickiness, disturbed anaphase-telophase and anaphase bridges were observed in anaphase-telophase cells, c-metaphase and binuclear cells were observed in other cells. A significant increase in DNA damage was also observed at all concentrations of ITO by the Comet assay. These results indicate that ITO exhibits genotoxic activity in A. cepa root meristematic cells.

Citing Articles

Contribution of toxicological pathology to occupational health: lung carcinogenicity of fibrous and particulate substances in rats.

Fukushima S, Kasai T, Senoh H, Umeda Y, Mine T, Sasaki T J Toxicol Pathol. 2023; 36(2):69-83.

PMID: 37101961 PMC: 10123297. DOI: 10.1293/tox.2022-0086.

Cyto-Genotoxic and Behavioral Effects of Flubendiamide in Root Cells, and Molecular Docking Studies.

Cigerci I, Liman R, Istifli E, Akyil D, Ozkara A, Bonciu E Int J Mol Sci. 2023; 24(2).

PMID: 36675079 PMC: 9861014. DOI: 10.3390/ijms24021565.

Cytotoxic and Genotoxic Evaluation of Biosynthesized Silver Nanoparticles Using on MCF-7 and HUVEC Cell Lines.

Alkan H, Cigerci I, Ali M, Hazman O, Liman R, Cola F Plants (Basel). 2022; 11(10).

PMID: 35631722 PMC: 9143030. DOI: 10.3390/plants11101293.

Nanomaterials Induced Genotoxicity in Plant: Methods and Strategies.

Marmiroli M, Marmiroli N, Pagano L Nanomaterials (Basel). 2022; 12(10).

PMID: 35630879 PMC: 9145990. DOI: 10.3390/nano12101658.

Cytogenetic Consequences of Food Industry Workers Occupationally Exposed to Cooking Oil Fumes (COFs).

Pappuswamy M, Meyyazhagan A, Balasubramanian B, Kuchi Bhotla H, Pushparaj K, Eswaran M Asian Pac J Cancer Prev. 2021; 22(11):3591-3599.

PMID: 34837917 PMC: 9068188. DOI: 10.31557/APJCP.2021.22.11.3591.

References

Chonan T, Taguchi O, Omae K . Interstitial pulmonary disorders in indium-processing workers. Eur Respir J. 2006; 29(2):317-24. DOI: 10.1183/09031936.00020306. View

Hamaguchi T, Omae K, Takebayashi T, Kikuchi Y, Yoshioka N, Nishiwaki Y . Exposure to hardly soluble indium compounds in ITO production and recycling plants is a new risk for interstitial lung damage. Occup Environ Med. 2007; 65(1):51-5. DOI: 10.1136/oem.2006.029124. View

Barberio A, Voltolini J, Mello M . Standardization of bulb and root sample sizes for the Allium cepa test. Ecotoxicology. 2011; 20(4):927-35. DOI: 10.1007/s10646-011-0602-8. View

Liu H, Chen C, Chen G, Lee L, Chen H . Relationship between indium exposure and oxidative damage in workers in indium tin oxide production plants. Int Arch Occup Environ Health. 2011; 85(4):447-53. DOI: 10.1007/s00420-011-0688-6. View

Duez P, Dehon G, Kumps A, Dubois J . Statistics of the Comet assay: a key to discriminate between genotoxic effects. Mutagenesis. 2003; 18(2):159-66. DOI: 10.1093/mutage/18.2.159. View

Kovalchuk O, Kovalchuk I, Arkhipov A, Telyuk P, Hohn B, Kovalchuk L . The Allium cepa chromosome aberration test reliably measures genotoxicity of soils of inhabited areas in the Ukraine contaminated by the Chernobyl accident. Mutat Res. 1998; 415(1-2):47-57. DOI: 10.1016/s1383-5718(98)00053-9. View

Asakura K, Satoh H, Chiba M, Okamoto M, Serizawa K, Nakano M . Genotoxicity studies of heavy metals: lead, bismuth, indium, silver and antimony. J Occup Health. 2009; 51(6):498-512. DOI: 10.1539/joh.l9080. View

Nakajima M, Mitsunaga K, Nakazawa K, Usami M . In vivo/in vitro study in rat embryos on indium-caused tail malformations. Reprod Toxicol. 2008; 25(4):426-32. DOI: 10.1016/j.reprotox.2008.04.008. View

Olive P, Banath J . The comet assay: a method to measure DNA damage in individual cells. Nat Protoc. 2007; 1(1):23-9. DOI: 10.1038/nprot.2006.5. View

10.

Liman R, Cigerci I, Ozturk N . Determination of genotoxic effects of Imazethapyr herbicide in Allium cepa root cells by mitotic activity, chromosome aberration, and comet assay. Pestic Biochem Physiol. 2015; 118:38-42. DOI: 10.1016/j.pestbp.2014.11.007. View

11.

Tice R, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H . Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen. 2000; 35(3):206-21. DOI: 10.1002/(sici)1098-2280(2000)35:3<206::aid-em8>3.0.co;2-j. View

12.

Shahin S . Induction of numerical chromosomal aberrations during DNA synthesis using the fungicides nimrod and rubigan-4 in root tips of Vicia faba L. Mutat Res. 1991; 261(3):169-76. DOI: 10.1016/0165-1218(91)90064-s. View

13.

Lison D, Laloy J, Corazzari I, Muller J, Rabolli V, Panin N . Sintered indium-tin-oxide (ITO) particles: a new pneumotoxic entity. Toxicol Sci. 2009; 108(2):472-81. DOI: 10.1093/toxsci/kfp014. View

14.

Homma T, Ueno T, Sekizawa K, Tanaka A, Hirata M . Interstitial pneumonia developed in a worker dealing with particles containing indium-tin oxide. J Occup Health. 2003; 45(3):137-9. DOI: 10.1539/joh.45.137. View

15.

Rucinska R, Sobkowiak R, Gwozdz E . Genotoxicity of lead in lupin root cells as evaluated by the comet assay. Cell Mol Biol Lett. 2004; 9(3):519-28. View

16.

Emerit J, Beaumont C, Trivin F . Iron metabolism, free radicals, and oxidative injury. Biomed Pharmacother. 2001; 55(6):333-9. DOI: 10.1016/s0753-3322(01)00068-3. View

17.

Kuriyama R, Sakai H . Role of tubulin-SH groups in polymerization to microtubules. Functional-SH groups in tubulin for polymerization. J Biochem. 1974; 76(3):651-4. DOI: 10.1093/oxfordjournals.jbchem.a130609. View

18.

Grant W . Higher plant assays for the detection of chromosomal aberrations and gene mutations-a brief historical background on their use for screening and monitoring environmental chemicals. Mutat Res. 1999; 426(2):107-12. DOI: 10.1016/s0027-5107(99)00050-0. View

19.

Tkalec M, Malaric K, Pavlica M, Pevalek-Kozlina B, Vidakovic-Cifrek Z . Effects of radiofrequency electromagnetic fields on seed germination and root meristematic cells of Allium cepa L. Mutat Res. 2008; 672(2):76-81. DOI: 10.1016/j.mrgentox.2008.09.022. View

20.

Lin R, Yang M, Li Y, Chang H, Kuan Y . Indium chloride-induced micronuclei via reactive oxygen species in Chinese hamster lung fibroblast V79 cells. Environ Toxicol. 2013; 28(10):595-600. DOI: 10.1002/tox.20755. View