Human Cellular Retinaldehyde-binding Protein Has Secondary Thermal 9-cis-retinal Isomerase Activity

Overview

Journal J Am Chem Soc

Publisher American Chemical Society

Specialty Chemistry

Date 2013 Dec 17

PMID 24328211

Citations 7

Authors

Christin S Bolze

Rachel E Helbling

Robin L Owen

Arwen R Pearson

Guillaume Pompidor

Florian Dworkowski

Martin R Fuchs

Julien Furrer

Marcin Golczak

Krzysztof Palczewski

Michele Cascella

Achim Stocker

Affiliations

Soon will be listed here.

Abstract

Cellular retinaldehyde-binding protein (CRALBP) chaperones 11-cis-retinal to convert opsin receptor molecules into photosensitive retinoid pigments of the eye. We report a thermal secondary isomerase activity of CRALBP when bound to 9-cis-retinal. UV/vis and (1)H NMR spectroscopy were used to characterize the product as 9,13-dicis-retinal. The X-ray structure of the CRALBP mutant R234W:9-cis-retinal complex at 1.9 Å resolution revealed a niche in the binding pocket for 9-cis-aldehyde different from that reported for 11-cis-retinal. Combined computational, kinetic, and structural data lead us to propose an isomerization mechanism catalyzed by a network of buried waters. Our findings highlight a specific role of water molecules in both CRALBP-assisted specificity toward 9-cis-retinal and its thermal isomerase activity yielding 9,13-dicis-retinal. Kinetic data from two point mutants of CRALBP support an essential role of Glu202 as the initial proton donor in this isomerization reaction.

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