Detection of Acute Toxoplasmosis in Pigs Using Loop-mediated Isothermal Amplification and Quantitative PCR

Overview

Journal Korean J Parasitol

Specialty Parasitology

Date 2013 Dec 12

PMID 24327785

Citations 8

Authors

Yanhua Wang

Guangxiang Wang

Delin Zhang

Hong Yin

Meng Wang

Affiliations

Soon will be listed here.

Abstract

A loop-mediated isothermal amplification (LAMP) assay allows rapid diagnosis of Toxoplasma gondii infection. In the present study, the LAMP assay was evaluated using blood from both naturally and experimentally infected pigs. The sensitivity of the LAMP assay was compared with that of Q-PCR. Both assays detected T. gondii in the blood of experimentally infected pigs, with 100% agreement. In infected blood samples, the parasite was detected as early as 2 days post-infection and reached a peak in 3-5 days. In 216 field serum samples, the detection rates of LAMP and Q-PCR assays were 6.9% and 7.8%, respectively. This result indicates that the sensitivity of the LAMP assay was slightly lower than that of the Q-PCR assay. However, the LAMP may be an attractive diagnostic method in conditions where sophisticated and expensive equipment is unavailable. This assay could be a powerful supplement to current diagnostic methods.

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