Oxytocin and CAMP Stimulate Monovalent Cation Movements Through a Ca2+-sensitive, Amiloride-insensitive Channel in the Apical Membrane of Toad Urinary Bladder

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1987 Jan 1

PMID 2432608

Citations 21

Authors

W Van Driessche

I Aelvoet

D Erlij

Affiliations

Soon will be listed here.

Abstract

The effects of oxytocin and cAMP on ion transport were investigated in toad urinary bladders incubated with Ca2+-free solutions on the apical side. Under these conditions both oxytocin and cAMP markedly stimulated the movements of Na+, K+, Rb+, Cs+, Li+, and NH4+ through a pathway that is insensitive to amiloride. The amiloride-insensitive currents were inhibited by the addition of Ca2+, Sr2+, or Mg2+ to the apical solution. The movement of the monovalent cations was associated with a spontaneous Lorentzian component in the power spectrum of the fluctuation in short-circuit current. The plateau of the Lorentzian component was enhanced by oxytocin and cAMP and was depressed by divalent cations. Methohexital inhibited the stimulation of monovalent cation movements caused by oxytocin. These findings suggest that oxytocin and cAMP activate at least two kinds of ionic channels in the apical membrane of toad urinary bladder: the well-known amiloride-sensitive channel and an amiloride-insensitive channel that allows the movement of several monovalent cations and is blocked by Ca2+ and other divalent cations.

Citing Articles

Atrial natriuretic peptide and cGMP activate sodium transport through PKA-dependent pathway in the urinary bladder of the Japanese tree frog.

Yamada T, Matsuda K, Uchiyama M J Comp Physiol B. 2005; 176(3):203-12.

PMID: 16320061 DOI: 10.1007/s00360-005-0041-z.

Effects of prostaglandin E2 on membrane voltage of the connecting tubule and cortical collecting duct from rabbits.

Shimizu T, Nakamura M, Yoshitomi K, Imai M J Physiol. 1993; 462:275-89.

PMID: 8331584 PMC: 1175301. DOI: 10.1113/jphysiol.1993.sp019555.

Ca(2+)-blockable, poorly selective cation channels in the apical membrane of amphibian epithelia. Tetracaine blocks the UO2(2+)-insensitive pathway.

Desmedt L, Simaels J, Van Driessche W J Gen Physiol. 1993; 101(1):103-16.

PMID: 7679716 PMC: 2216756. DOI: 10.1085/jgp.101.1.103.

Cell swelling activates a poorly selective monovalent cation channel in the apical membrane of toad urinary bladder.

Van Driessche W, Erlij D Pflugers Arch. 1994; 428(1):1-8.

PMID: 7526336 DOI: 10.1007/BF00374745.

K+ efflux in NIH mouse 3T3 cells and transformed derivatives: dependence on extracellular Ca2+ and phorbol esters.

Lubin M Proc Natl Acad Sci U S A. 1988; 85(14):5097-101.

PMID: 3393533 PMC: 281695. DOI: 10.1073/pnas.85.14.5097.

References

Van Driessche W, Lindemann B . Low-noise amplification of voltage and current fluctuations arising in epithelia. Rev Sci Instrum. 1978; 49(1):53-7. View

Reuter H, Scholz H . The regulation of the calcium conductance of cardiac muscle by adrenaline. J Physiol. 1977; 264(1):49-62. PMC: 1307747. DOI: 10.1113/jphysiol.1977.sp011657. View

Macknight A, Dibona D, Leaf A . Sodium transport across toad urinary bladder: a model "tight" epithelium. Physiol Rev. 1980; 60(3):615-715. DOI: 10.1152/physrev.1980.60.3.615. View

Van Driessche W, Zeiske W . Ba2+-induced conductance fluctuations of spontaneously fluctuating K+ channels in the apical membrane of frog skin (Rana temporaria). J Membr Biol. 1980; 56(1):31-42. DOI: 10.1007/BF01869349. View

Schoen H, Erlij D . Basolateral membrane responses to transport modifiers in the frog skin epithelium. Pflugers Arch. 1985; 405 Suppl 1:S33-8. DOI: 10.1007/BF00581777. View

Hagiwara S, Byerly L . Calcium channel. Annu Rev Neurosci. 1981; 4:69-125. DOI: 10.1146/annurev.ne.04.030181.000441. View

Stetson D, Lewis S, Alles W, Wade J . Evaluation by capacitance measurements of antidiuretic hormone induced membrane area changes in toad bladder. Biochim Biophys Acta. 1982; 689(2):267-74. DOI: 10.1016/0005-2736(82)90259-0. View

de Sousa R, Grosso A . Osmotic water flow across the abdominal skin of the toad bufo marinus: effect of vasopressin and isoprenaline. J Physiol. 1982; 329:281-96. PMC: 1224780. DOI: 10.1113/jphysiol.1982.sp014303. View

Van Driessche W, Erlij D . Noise analysis of inward and outward Na+ currents across the apical border of ouabain-treated frog skin. Pflugers Arch. 1983; 398(3):179-88. DOI: 10.1007/BF00657149. View

10.

Erne P, Burgisser E, Buhler F, Dubach B, Kuhnis H, Meier M . Enhancement of calcium influx in human platelets by CGP 28392, a novel dihydropyridine. Biochem Biophys Res Commun. 1984; 118(3):842-7. DOI: 10.1016/0006-291x(84)91471-2. View

11.

Hess P, Tsien R . Mechanism of ion permeation through calcium channels. Nature. 1984; 309(5967):453-6. DOI: 10.1038/309453a0. View

12.

Almers W, McCleskey E, Palade P . A non-selective cation conductance in frog muscle membrane blocked by micromolar external calcium ions. J Physiol. 1984; 353:565-83. PMC: 1193322. DOI: 10.1113/jphysiol.1984.sp015351. View

13.

Lee K, Tsien R . High selectivity of calcium channels in single dialysed heart cells of the guinea-pig. J Physiol. 1984; 354:253-72. PMC: 1193410. DOI: 10.1113/jphysiol.1984.sp015374. View

14.

Brown A, Kunze D, Yatani A . The agonist effect of dihydropyridines on Ca channels. Nature. 1984; 311(5986):570-2. DOI: 10.1038/311570a0. View

15.

McCleskey E, Almers W . The Ca channel in skeletal muscle is a large pore. Proc Natl Acad Sci U S A. 1985; 82(20):7149-53. PMC: 391328. DOI: 10.1073/pnas.82.20.7149. View

16.

Van Driessche W, Zeiske W . Ionic channels in epithelial cell membranes. Physiol Rev. 1985; 65(4):833-903. DOI: 10.1152/physrev.1985.65.4.833. View

17.

Van Driessche W, Zeiske W . Ca2+-sensitive, spontaneously fluctuating, cation channels in the apical membrane of the adult frog skin epithelium. Pflugers Arch. 1985; 405(3):250-9. DOI: 10.1007/BF00582569. View

18.

Sperelakis N, Schneider J . A metabolic control mechanism for calcium ion influx that may protect the ventricular myocardial cell. Am J Cardiol. 1976; 37(7):1079-85. DOI: 10.1016/0002-9149(76)90428-8. View

19.

Levine S, Levine R, Worthington R, Hays R . Selective inhibition of osmotic water flow by general anesthetics to toad urinary bladder. J Clin Invest. 1976; 58(4):980-8. PMC: 333262. DOI: 10.1172/JCI108552. View

20.

Kachadorian W, Levine S, Wade J, Di Scala V, Hays R . Relationship of aggregated intramembranous particles to water permeability in vasopressin-treated toad urinary bladder. J Clin Invest. 1977; 59(3):576-81. PMC: 333395. DOI: 10.1172/JCI108673. View