The Mitigating Effect of Citrullus Colocynthis (L.) Fruit Extract Against Genotoxicity Induced by Cyclophosphamide in Mice Bone Marrow Cells

Overview

Journal ScientificWorldJournal

Publisher Wiley

Specialty Biology

Date 2013 Dec 11

PMID 24324391

Citations 5

Authors

Mohammad Shokrzadeh

Aroona Chabra

Farshad Naghshvar

Amirhossein Ahmadi

Affiliations

Soon will be listed here.

Abstract

Possible genoprotective effect of Citrullus colocynthis (L.) (CCT) fruits extract against cyclophosphamide- (CP-)induced DNA damage in mice bone marrow cells was evaluated using micronucleus assay, as an index of induced chromosomal damage. Mice were preadministered with different doses of CCT via intraperitoneal injection for 7 consecutive days followed by injection with CP (70 mg/kg b.w.) 1 hr after the last injection of CCT. After 24 hr, mice were scarified to evaluate the frequency of micronucleated polychromatic erythrocytes (MnPCEs). In addition, the number of polychromatic erythrocytes (PCEs) among 1000 normochromatic erythrocytes (NCEs) per animal was recorded to evaluate bone marrow. Pretreatment with CCT significantly reduced the number of MnPCEs induced by CP in bone marrow cells (P < 0.0001). At 200 mg/kg, CCT had a maximum chemoprotective effect and reduced the number of MnPCEs by 6.37-fold and completely normalized the mitotic activity. CCT also led to marked proliferation and hypercellularity of immature myeloid elements after mice were treated with CP and mitigated the bone marrow suppression. Our study revealed that CCT has an antigenotoxic effect against CP-induced oxidative DNA damage in mice. Therefore, it could be used concomitantly as a supplement to protect people undergoing chemotherapy.

Citing Articles

Evaluation of the Antigenotoxic Potential of Two Types of Chayote () Juices.

Madrigal-Santillan E, Portillo-Reyes J, Morales-Gonzalez J, Garcia-Melo L, Serra-Perez E, Vidovic K Plants (Basel). 2024; 13(15).

PMID: 39124250 PMC: 11314257. DOI: 10.3390/plants13152132.

Alogliptin: a novel approach against cyclophosphamide-induced hepatic injury via modulating SIRT1/FoxO1 pathway.

Salama R, Mohamed A, Hamed N, Ata R, NourelDeen A, Hassan M Toxicol Res (Camb). 2020; 9(4):561-568.

PMID: 32905193 PMC: 7467234. DOI: 10.1093/toxres/tfaa059.

Evaluation of genotoxicity of Forssk. extract on mice bone marrow cells .

Mohamed H, Aly M Toxicol Rep. 2018; 5:625-631.

PMID: 29854632 PMC: 5977870. DOI: 10.1016/j.toxrep.2018.05.007.

Antigenotoxic effects of a polyherbal drug septilin against the genotoxicity of cyclophosphamide in mice.

Shruthi S, Vijayalaxmi K Toxicol Rep. 2017; 3:563-571.

PMID: 28959580 PMC: 5615931. DOI: 10.1016/j.toxrep.2016.07.001.

Watermelon (Citrullus lanatus (Thunb.) Matsum. and Nakai) juice modulates oxidative damage induced by low dose X-ray in mice.

Mohammad M, Mohamed M, Zakaria A, Abdul Razak H, Md Saad W Biomed Res Int. 2014; 2014:512834.

PMID: 24877107 PMC: 4020559. DOI: 10.1155/2014/512834.

References

Anderson M . Determination of glutathione and glutathione disulfide in biological samples. Methods Enzymol. 1985; 113:548-55. DOI: 10.1016/s0076-6879(85)13073-9. View

Selvakumar E, Prahalathan C, Mythili Y, Varalakshmi P . Mitigation of oxidative stress in cyclophosphamide-challenged hepatic tissue by DL-alpha-lipoic acid. Mol Cell Biochem. 2005; 272(1-2):179-85. DOI: 10.1007/s11010-005-7322-4. View

Hosseinimehr S, Ahmadi A, Mahmoudzadeh A, Mohamadifar S . Radioprotective effects of Daflon against genotoxicity induced by gamma irradiation in human cultured lymphocytes. Environ Mol Mutagen. 2009; 50(9):749-52. DOI: 10.1002/em.20499. View

Schmid W . The micronucleus test. Mutat Res. 1975; 31(1):9-15. DOI: 10.1016/0165-1161(75)90058-8. View

Griffith O . Biologic and pharmacologic regulation of mammalian glutathione synthesis. Free Radic Biol Med. 1999; 27(9-10):922-35. DOI: 10.1016/s0891-5849(99)00176-8. View

Arami S, Ahmadi A, Haeri S . The radioprotective effects of Origanum vulgare extract against genotoxicity induced by (131)I in human blood lymphocyte. Cancer Biother Radiopharm. 2013; 28(3):201-6. DOI: 10.1089/cbr.2012.1284. View

Das U, Mallick M, Debnath J, Ghosh D . Protective effect of ascorbic acid on cyclophosphamide- induced testicular gametogenic and androgenic disorders in male rats. Asian J Androl. 2002; 4(3):201-7. View

Abdel-Barry J, Tariq Mohammeda S . The hypoglycaemic and antihyperglycaemic effect of citrullus colocynthis fruit aqueous extract in normal and alloxan diabetic rabbits. J Ethnopharmacol. 2000; 71(1-2):325-30. DOI: 10.1016/s0378-8741(99)00215-9. View

Fraiser L, Kanekal S, Kehrer J . Cyclophosphamide toxicity. Characterising and avoiding the problem. Drugs. 1991; 42(5):781-95. DOI: 10.2165/00003495-199142050-00005. View

10.

Baumann F, Preiss R . Cyclophosphamide and related anticancer drugs. J Chromatogr B Biomed Sci Appl. 2002; 764(1-2):173-92. DOI: 10.1016/s0378-4347(01)00279-1. View

11.

Al-Ghaithi F, El-Ridi M, Adeghate E, Amiri M . Biochemical effects of Citrullus colocynthis in normal and diabetic rats. Mol Cell Biochem. 2004; 261(1-2):143-9. DOI: 10.1023/b:mcbi.0000028749.63101.cc. View

12.

Hosseinimehr S, Ahmadashrafi S, Naghshvar F, Ahmadi A, Ehasnalavi S, Tanha M . Chemoprotective effects of Zataria multiflora against genotoxicity induced by cyclophosphamide in mice bone marrow cells. Integr Cancer Ther. 2010; 9(2):219-23. DOI: 10.1177/1534735409360361. View

13.

Aune D, Stefani E, Ronco A, Boffetta P, Deneo-Pellegrini H, Acosta G . Fruits, vegetables and the risk of cancer: a multisite case-control study in Uruguay. Asian Pac J Cancer Prev. 2009; 10(3):419-28. View

14.

Hosseinimehr S, Mahmoudzadeh A, Ahmadi A, Ashrafi S, Shafaghati N, Hedayati N . The radioprotective effect of Zataria multiflora against genotoxicity induced by γ irradiation in human blood lymphocytes. Cancer Biother Radiopharm. 2011; 26(3):325-9. DOI: 10.1089/cbr.2010.0896. View

15.

Ellman G . Tissue sulfhydryl groups. Arch Biochem Biophys. 1959; 82(1):70-7. DOI: 10.1016/0003-9861(59)90090-6. View

16.

Moore F, Urda G, Krishna G, Theiss J . An in vivo/in vitro method for assessing micronucleus and chromosome aberration induction in rat bone marrow and spleen. 1. Studies with cyclophosphamide. Mutat Res. 1995; 335(2):191-9. DOI: 10.1016/0165-1161(95)90055-1. View

17.

Ghosh D, Das U, Ghosh S, Mallick M, Debnath J . Testicular gametogenic and steroidogenic activities in cyclophosphamide treated rat: a correlative study with testicular oxidative stress. Drug Chem Toxicol. 2002; 25(3):281-92. DOI: 10.1081/dct-120005891. View

18.

Tice R, Erexson G, Shelby M . The induction of micronucleated polychromatic erythrocytes in mice using single and multiple treatments. Mutat Res. 1990; 234(3-4):187-93. DOI: 10.1016/0165-1161(90)90014-f. View

19.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

20.

Palm F, Cederberg J, Hansell P, Liss P, Carlsson P . Reactive oxygen species cause diabetes-induced decrease in renal oxygen tension. Diabetologia. 2003; 46(8):1153-60. DOI: 10.1007/s00125-003-1155-z. View