Restricted Light Chain Subgroup Expression on Human Rheumatoid Factor Paraproteins Determined by Monoclonal Antibodies

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1986 Nov 1

PMID 2432001

Citations 15

Authors

R A Mageed

M R Walker

R Jefferis

Affiliations

Soon will be listed here.

Abstract

Two hybridoma antibodies specific for determinants on the V kappa light chain subgroup have been produced and characterized. Antibodies C7 and B12 reacted with distinct V kappa epitopes irrespective of association with heavy chain class or subclass. Epitopes recognized by C7 and B12 were expressed on the light chain of IgG, IgA, and IgM and Bence-Jones paraproteins from the V kappa subgroup. However, a preferential association of both epitopes with IgM RF paraproteins was demonstrated. Hybridomas C7 and B12 reacted with 12/12 and 10/12 IgM RF paraproteins, respectively, but only with 3/6 IgM paraproteins, with no RF activity. Both epitopes C7 and B12, were immunodominant and conformation dependent, being detected by HA, HAI and ELISA on intact light chain but not isolated VK.

Citing Articles

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References

Newcomb R, Normansell D, STANWORTH D . A structural study of human exocrine IgA globulin. J Immunol. 1968; 101(5):905-14. View

Farris M, Hardie D, De Lange G, Jefferis R . Immunogenic and antigenic epitopes of immunoglobulins. X: Monoclonal antibodies specific for human IgA, the IgA1 and IgA2 subclasses and an nA2m(2) iso-allotypic epitope. Vox Sang. 1985; 48(2):116-21. DOI: 10.1111/j.1423-0410.1985.tb00155.x. View

Solomon A, McLaughlin C . Bence Jones proteins and light chains of immunoglobulins. IV. Immunochemical differentiation among proteins within each of the three established kappa-chain classes. J Immunol. 1971; 106(1):120-7. View

KUNKEL H, Winchester R, Joslin F, Capra J . Similarities in the light chains of anti-gamma-globulins showing cross-idiotypic specificities. J Exp Med. 1974; 139(1):128-36. PMC: 2139505. DOI: 10.1084/jem.139.1.128. View

Capra J, Kehoe J . Hypervariable regions, idiotypy, and the antibody-combining site. Adv Immunol. 1975; 20:1-40. DOI: 10.1016/s0065-2776(08)60205-9. View

Kohler G, Milstein C . Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975; 256(5517):495-7. DOI: 10.1038/256495a0. View

Feizi T, LECOMTE J, Childs R, Solomon A . Kappa chain (V kappa III) subgroup-related activity in an idiotypic anti-cold agglutinin serum. Scand J Immunol. 1976; 5(6-7):629-36. DOI: 10.1111/j.1365-3083.1976.tb03012.x. View

LING N, Bishop S, Jefferis . Use of antibody-coated red cells for the sensitive detection of antigen and in rosette tests for cells bearing surface immunoglobulins. J Immunol Methods. 1977; 15(3):279-89. DOI: 10.1016/0022-1759(77)90065-5. View

KABAT E, Wu T, Bilofsky H . Variable region genes for the immunoglobulin framework are assembled from small segments of DNA--a hypothesis. Proc Natl Acad Sci U S A. 1978; 75(5):2429-33. PMC: 392567. DOI: 10.1073/pnas.75.5.2429. View

10.

Lowe J, Hardie D, Jefferis R, LING N, Drysdale P, Richardson P . Properties of monoclonal antibodies to human immunoglobulin kappa and lambda chains. Immunology. 1981; 42(4):649-59. PMC: 1458254. View

11.

Albutt E, Hawker P, Hine K, NORTHAM B . Diagnosis of gamma heavy-chain disease. Ann Clin Biochem. 1981; 18(Pt 4):207-10. DOI: 10.1177/000456328101800403. View

12.

Lowe J, Bird P, Hardie D, Jefferis R, LING N . Monoclonal antibodies (McAbs) to determinants on human gamma chains: properties of antibodies showing subclass restriction or subclass specificity. Immunology. 1982; 47(2):329-36. PMC: 1555448. View

13.

Jaafar M, Lowe J, LING N, Jefferis R . Immunogenic and antigenic epitopes of immunoglobulins--V. Reactivity of a panel of monoclonal antibodies with sub-fragments of human Fc gamma and abnormal paraproteins having deletions. Mol Immunol. 1983; 20(6):679-86. DOI: 10.1016/0161-5890(83)90012-3. View

14.

Ledford D, Goni F, Pizzolato M, FRANKLIN E, Solomon A, Frangione B . Preferential association of kappa IIIb light chains with monoclonal human IgM kappa autoantibodies. J Immunol. 1983; 131(3):1322-5. View

15.

Greenstein J, Solomon A, Abraham G . Monoclonal antibodies reactive with idiotypic and variable-region specific determinants on human immunoglobulins. Immunology. 1984; 51(1):17-25. PMC: 1454402. View

16.

Carson D, Fong S . A common idiotope on human rheumatoid factors identified by a hybridoma antibody. Mol Immunol. 1983; 20(10):1081-7. DOI: 10.1016/0161-5890(83)90116-5. View

17.

Walker M, Hardie D, Lowe J, LING N, De Lange G, Jefferis R . Immunogenicity and antigenicity of immunoglobulins. XII. Intact light chain and heavy chain isotype-restricted Vk-associated epitopes. Immunology. 1985; 55(2):205-11. PMC: 1453615. View

18.

Jefferis R, Reimer C, Skvaril F, De Lange G, LING N, Lowe J . Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: results of an IUIS/WHO collaborative study. Immunol Lett. 1985; 10(3-4):223-52. DOI: 10.1016/0165-2478(85)90082-3. View

19.

Goni F, Chen P, Pons-Estel B, Carson D, Frangione B . Sequence similarities and cross-idiotypic specificity of L chains among human monoclonal IgM kappa with anti-gamma-globulin activity. J Immunol. 1985; 135(6):4073-9. View

20.

Plaut A, TOMASI Jr T . Immunoglobulin M: pentameric Fcmu fragments released by trypsin at higher temperatures. Proc Natl Acad Sci U S A. 1970; 65(2):318-22. PMC: 282904. DOI: 10.1073/pnas.65.2.318. View