Urokinase Plasminogen Activator System in Synovial Fibroblasts from Osteoarthritis Patients: Modulation by Inflammatory Mediators and Neuropeptides

Overview

Journal J Mol Neurosci

Specialties Molecular Biology
Neurology

Date 2013 Dec 10

PMID 24318839

Citations 13

Authors

Selene Perez-Garcia

Mar Carrion

Rebeca Jimeno

Ana M Ortiz

Isidoro Gonzalez-Alvaro

Julian Fernandez

Rosa P Gomariz

Yasmina Juarranz

Affiliations

Soon will be listed here.

Abstract

Plasminogen activators are specific proteolytic enzymes implicated in a variety of basic biological processes. The expression of the urokinase plasminogen activator system components is increased in some human diseases, including osteoarthritis. We sought to study the effect of two components of the inflamed synovial microenvironment on this system, IL-1β and fibronectin fragments, elucidating whether corticotropin-releasing factor (CRF) and vasoactive intestinal peptide (VIP) neuropeptides modulate it, and analyzing the physiological consequences in joint destruction by measuring matrix metalloproteinases-9 and metalloproteinases-13 levels in osteoarthritis fibroblast-like synoviocytes. We showed that IL-1β and fibronectin fragments stimulated urokinase system contributing to the perpetuation of the destructive cascade in joint. VIP modulated, even at constitutive level, this system, also counteracting the effect of both inflammatory stimuli. However, CRF seemed to be ineffective in controlling the production of these proteinases. Moreover, VIP was able to reduce the constitutive expression of matrix metalloproteinase-13 and the levels of both matrix metalloproteinases after stimulation with the pro-inflammatory stimuli. Our results suggest that the presence of early and later inflammatory mediators, such as IL-1β and fibronectin fragments, increases the urokinase system and the matrix metalloproteinases levels. Whereas CRF did not affect this system, VIP counteracts these actions supporting its therapeutic potential for the treatment of osteoarthritis.

Citing Articles

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Lu H, Lu J, Lee C, Peng Y, Lee H, Chu Y J Clin Med. 2021; 10(19).

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Proteomic Analysis of Synovial Fibroblasts and Articular Chondrocytes Co-Cultures Reveals Valuable VIP-Modulated Inflammatory and Degradative Proteins in Osteoarthritis.

Perez-Garcia S, Calamia V, Hermida-Gomez T, Gutierrez-Canas I, Carrion M, Villanueva-Romero R Int J Mol Sci. 2021; 22(12).

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