Three Tightly Linked Genes Encoding Human Type I Keratins: Conservation of Sequence in the 5'-untranslated Leader and 5'-upstream Regions of Coexpressed Keratin Genes

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1986 Feb 1

PMID 2431270

Citations 21

Authors

A Raychaudhury

D Marchuk

M Lindhurst

E Fuchs

Affiliations

Soon will be listed here.

Abstract

We have isolated and subcloned three separate segments of human DNA which share strong sequence homology with a previously sequenced gene encoding a type I keratin, K14 (50 kilodaltons). Restriction endonuclease mapping has demonstrated that these three genes are tightly linked chromosomally, whereas the K14 gene appears to be separate. As judged by positive hybridization-translation and Northern blot analyses, the central linked gene encodes a keratin, K17, which is expressed in abundance with K14 and two other type I keratins in cultured human epidermal cells. None of these other epidermal keratin mRNAs appears to be generated from the K17 gene through differential splicing of its transcript. The sequence of the K17 gene reveals striking homologies not only with the coding portions and intron positions of the K14 gene, but also with its 5'-noncoding and 5'-upstream sequences. These similarities may provide an important clue in elucidating the molecular mechanisms underlying the coexpression of the two genes.

Citing Articles

Intermediate filament expression in prostate cancer.

Nagle R Cancer Metastasis Rev. 1996; 15(4):473-82.

PMID: 9034604 DOI: 10.1007/BF00054013.

Differential expression of cytokeratin mRNA and protein in normal prostate, prostatic intraepithelial neoplasia, and invasive carcinoma.

Yang Y, Hao J, Liu X, Dalkin B, Nagle R Am J Pathol. 1997; 150(2):693-704.

PMID: 9033282 PMC: 1858264.

Mutation of human keratin 18 in association with cryptogenic cirrhosis.

Ku N, Wright T, Terrault N, Gish R, Omary M J Clin Invest. 1997; 99(1):19-23.

PMID: 9011570 PMC: 507762. DOI: 10.1172/JCI119127.

A review of intermediate filament biology and their use in pathologic diagnosis.

Nagle R Mol Biol Rep. 1994; 19(1):3-21.

PMID: 8170466 DOI: 10.1007/BF00987318.

The human mid-size neurofilament subunit: a repeated protein sequence and the relationship of its gene to the intermediate filament gene family.

Myers M, Lazzarini R, Lee V, SCHLAEPFER W, Nelson D EMBO J. 1987; 6(6):1617-26.

PMID: 3608989 PMC: 553533. DOI: 10.1002/j.1460-2075.1987.tb02409.x.

References

McLachlan A, Stewart M . Tropomyosin coiled-coil interactions: evidence for an unstaggered structure. J Mol Biol. 1975; 98(2):293-304. DOI: 10.1016/s0022-2836(75)80119-7. View

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

Tyner A, Eichman M, Fuchs E . The sequence of a type II keratin gene expressed in human skin: conservation of structure among all intermediate filament genes. Proc Natl Acad Sci U S A. 1985; 82(14):4683-7. PMC: 390450. DOI: 10.1073/pnas.82.14.4683. View

Steinert P, Steven A, Roop D . The molecular biology of intermediate filaments. Cell. 1985; 42(2):411-20. DOI: 10.1016/0092-8674(85)90098-4. View

Steinert P, Idler W . The polypeptide composition of bovine epidermal alpha-keratin. Biochem J. 1975; 151(3):603-14. PMC: 1172408. DOI: 10.1042/bj1510603. View

Pelham H, Jackson R . An efficient mRNA-dependent translation system from reticulocyte lysates. Eur J Biochem. 1976; 67(1):247-56. DOI: 10.1111/j.1432-1033.1976.tb10656.x. View

Steinert P, Idler W, Zimmerman S . Self-assembly of bovine epidermal keratin filaments in vitro. J Mol Biol. 1976; 108(3):547-67. DOI: 10.1016/s0022-2836(76)80136-2. View

Benton W, Davis R . Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977; 196(4286):180-2. DOI: 10.1126/science.322279. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

10.

Sun T, Green H . Keratin filaments of cultured human epidermal cells. Formation of intermolecular disulfide bonds during terminal differentiation. J Biol Chem. 1978; 253(6):2053-60. View

11.

Garnier J, Osguthorpe D, Robson B . Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. J Mol Biol. 1978; 120(1):97-120. DOI: 10.1016/0022-2836(78)90297-8. View

12.

de Jong W, Zweers A, Cohen L . Influence of single amino acid substitutions on electrophoretic mobility of sodium dodecyl sulfate-protein complexes. Biochem Biophys Res Commun. 1978; 82(2):532-9. DOI: 10.1016/0006-291x(78)90907-5. View

13.

Steinert P . Structure of the three-chain unit of the bovine epidermal keratin filament. J Mol Biol. 1978; 123(1):49-70. DOI: 10.1016/0022-2836(78)90376-5. View

14.

McLachlan A . Coiled coil formation and sequence regularities in the helical regions of alpha-keratin. J Mol Biol. 1978; 124(1):297-304. DOI: 10.1016/0022-2836(78)90163-8. View

15.

Chou P, Fasman G . Prediction of the secondary structure of proteins from their amino acid sequence. Adv Enzymol Relat Areas Mol Biol. 1978; 47:45-148. DOI: 10.1002/9780470122921.ch2. View

16.

Fuchs E, Green H . The expression of keratin genes in epidermis and cultured epidermal cells. Cell. 1978; 15(3):887-97. DOI: 10.1016/0092-8674(78)90273-8. View

17.

Fuchs E, Green H . Multiple keratins of cultured human epidermal cells are translated from different mRNA molecules. Cell. 1979; 17(3):573-82. DOI: 10.1016/0092-8674(79)90265-4. View

18.

Fuchs E, Green H . Changes in keratin gene expression during terminal differentiation of the keratinocyte. Cell. 1980; 19(4):1033-42. DOI: 10.1016/0092-8674(80)90094-x. View

19.

Thomas P . Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980; 77(9):5201-5. PMC: 350025. DOI: 10.1073/pnas.77.9.5201. View

20.

Matsui T, Segall J, Weil P, Roeder R . Multiple factors required for accurate initiation of transcription by purified RNA polymerase II. J Biol Chem. 1980; 255(24):11992-6. View