The Purification and Properties of Some Less Common Allotypes of the Fourth Component of Human Complement

Overview

Journal Immunogenetics

Specialties Allergy & Immunology
Genetics

Date 1986 Jan 1

PMID 2430883

Citations 14

Authors

A W DODDS

S K Law

R R Porter

Affiliations

Soon will be listed here.

Abstract

Human complement component C4 is coded by two genes situated between HLA-D and HLA-B. Both genes are highly polymorphic; C4-A gene products normally carry the blood group antigen Rodgers and C4-B proteins usually carry the Chido antigen. Using a monoclonal antibody which binds Rodgers-positive and Chido-positive proteins with different affinities, we have purified a number of less common C4 allotypes and compared their properties. All C4-B allotypes tested have similar specific hemolytic activities and binding efficiencies to small molecules. All C4-A proteins tested had similar binding to small molecules and hemolytic activities except for the C4-A6 proteins from two individuals with different extended haplotypes, both of which had identical hemolytic activities and much lower ones than other C4-A allotypes. Two allotypes, C4-A1, Rodgers-negative but Chido-positive, and C4-B5, Chido-negative but probably Rodgers-positive, were found to behave as typical C4-A and C4-B proteins, respectively, apart from the switch in their antigenic properties.

Citing Articles

Complement C4, Infections, and Autoimmune Diseases.

Wang H, Liu M Front Immunol. 2021; 12:694928.

PMID: 34335607 PMC: 8317844. DOI: 10.3389/fimmu.2021.694928.

Deficiencies of human complement component C4A and C4B and heterozygosity in length variants of RP-C4-CYP21-TNX (RCCX) modules in caucasians. The load of RCCX genetic diversity on major histocompatibility complex-associated disease.

Blanchong C, Zhou B, Rupert K, Chung E, Jones K, Sotos J J Exp Med. 2000; 191(12):2183-96.

PMID: 10859342 PMC: 2193198. DOI: 10.1084/jem.191.12.2183.

The internal thioester and the covalent binding properties of the complement proteins C3 and C4.

Law S, DODDS A Protein Sci. 1997; 6(2):263-74.

PMID: 9041627 PMC: 2143658. DOI: 10.1002/pro.5560060201.

Covalent binding properties of the human complement protein C4 and hydrolysis rate of the internal thioester upon activation.

Sepp A, DODDS A, Anderson M, Campbell R, Willis A, Law S Protein Sci. 1993; 2(5):706-16.

PMID: 8495193 PMC: 2142499. DOI: 10.1002/pro.5560020502.

The thioester and isotypic sites of complement component C4 in sheep and cattle.

Ren X, DODDS A, Law S Immunogenetics. 1993; 37(2):120-8.

PMID: 8423050 DOI: 10.1007/BF00216835.

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