CCNG2 Expression is Downregulated in Colorectal Carcinoma and Its Clinical Significance

Overview

Journal Tumour Biol

Publisher Sage Publications

Specialty Oncology

Date 2013 Dec 6

PMID 24307622

Citations 8

Authors

G G Sun

J Zhang

W N Hu

Affiliations

Soon will be listed here.

Abstract

This study aimed to analyze the expression, clinical significance of cyclin G2 (CCNG2) in colorectal carcinoma, and the biological effect in its cell line by CCNG2 overexpression. Immunohistochemistry and Western blot were used to analyze CCNG2 protein expression in colorectal cancer and to study the influence of the upregulated expression of CCNG2 that might be found on SW480 cell biological effect. We found that the level of CCNG2 protein expression was significantly lower in colorectal cancer tissue than normal tissues (P < 0.05). The level of CCNG2 was correlated with T stages, lymph node metastasis, clinic stage, and histological grade (P < 0.05). Loss of CCNG2 expression correlated significantly with poor overall survival time by Kaplan-Meier analysis (P < 0.05). The result of biological function has shown that SW480 cell-transfected CCNG2 had a lower survival fraction, higher percentage of the G0/G1 phases, and lower CDK2 protein expression compared with SW480 cell-untransfected CCNG2 (P < 0.05).

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References

Horne M, Donaldson K, Goolsby G, Tran D, Mulheisen M, Hell J . Cyclin G2 is up-regulated during growth inhibition and B cell antigen receptor-mediated cell cycle arrest. J Biol Chem. 1997; 272(19):12650-61. DOI: 10.1074/jbc.272.19.12650. View

Bennin D, Don A, Brake T, McKenzie J, Rosenbaum H, Ortiz L . Cyclin G2 associates with protein phosphatase 2A catalytic and regulatory B' subunits in active complexes and induces nuclear aberrations and a G1/S phase cell cycle arrest. J Biol Chem. 2002; 277(30):27449-67. DOI: 10.1074/jbc.M111693200. View

van Duijn P, Ziel-van der Made A, van der Korput J, Trapman J . PTEN-mediated G1 cell-cycle arrest in LNCaP prostate cancer cells is associated with altered expression of cell-cycle regulators. Prostate. 2009; 70(2):135-46. DOI: 10.1002/pros.21045. View

Bates S, Rowan S, Vousden K . Characterisation of human cyclin G1 and G2: DNA damage inducible genes. Oncogene. 1996; 13(5):1103-9. View

Martinez-Gac L, Marques M, Garcia Z, Campanero M, Carrera A . Control of cyclin G2 mRNA expression by forkhead transcription factors: novel mechanism for cell cycle control by phosphoinositide 3-kinase and forkhead. Mol Cell Biol. 2004; 24(5):2181-9. PMC: 350549. DOI: 10.1128/MCB.24.5.2181-2189.2004. View

Chen J, Yusuf I, Andersen H, Fruman D . FOXO transcription factors cooperate with delta EF1 to activate growth suppressive genes in B lymphocytes. J Immunol. 2006; 176(5):2711-21. DOI: 10.4049/jimmunol.176.5.2711. View

Le X, Arachchige-Don A, Mao W, Horne M, Bast Jr R . Roles of human epidermal growth factor receptor 2, c-jun NH2-terminal kinase, phosphoinositide 3-kinase, and p70 S6 kinase pathways in regulation of cyclin G2 expression in human breast cancer cells. Mol Cancer Ther. 2007; 6(11):2843-57. DOI: 10.1158/1535-7163.MCT-07-0109. View

Xu G, Bernaudo S, Fu G, Lee D, Yang B, Peng C . Cyclin G2 is degraded through the ubiquitin-proteasome pathway and mediates the antiproliferative effect of activin receptor-like kinase 7. Mol Biol Cell. 2008; 19(11):4968-79. PMC: 2575152. DOI: 10.1091/mbc.e08-03-0259. View

Horne M, Goolsby G, Donaldson K, Tran D, Neubauer M, Wahl A . Cyclin G1 and cyclin G2 comprise a new family of cyclins with contrasting tissue-specific and cell cycle-regulated expression. J Biol Chem. 1996; 271(11):6050-61. DOI: 10.1074/jbc.271.11.6050. View

10.

Kim Y, Shintani S, Kohno Y, Zhang R, Wong D . Cyclin G2 dysregulation in human oral cancer. Cancer Res. 2004; 64(24):8980-6. DOI: 10.1158/0008-5472.CAN-04-1926. View

11.

Choi M, Noh J, An J, Hong S, Park S, Baik Y . Expression levels of cyclin G2, but not cyclin E, correlate with gastric cancer progression. J Surg Res. 2009; 157(2):168-74. DOI: 10.1016/j.jss.2008.06.020. View

12.

Ahmed S, Al-Saigh S, Matthews J . FOXA1 is essential for aryl hydrocarbon receptor-dependent regulation of cyclin G2. Mol Cancer Res. 2012; 10(5):636-48. DOI: 10.1158/1541-7786.MCR-11-0502. View

13.

Don A, Dallapiazza R, Bennin D, Brake T, Cowan C, Horne M . Cyclin G2 is a centrosome-associated nucleocytoplasmic shuttling protein that influences microtubule stability and induces a p53-dependent cell cycle arrest. Exp Cell Res. 2006; 312(20):4181-204. PMC: 1862360. DOI: 10.1016/j.yexcr.2006.09.023. View

14.

Cellai C, Laurenzana A, Bianchi E, Sdelci S, Manfredini R, Vannucchi A . Mechanistic insight into WEB-2170-induced apoptosis in human acute myelogenous leukemia cells: the crucial role of PTEN. Exp Hematol. 2009; 37(10):1176-1185.e21. DOI: 10.1016/j.exphem.2009.07.002. View

15.

Dimofte G, Tarcoveanu E, Tarasi M, Panait C, Lozneanu G, Nicolescu S . Mean number of lymph nodes in colonic cancer specimen: possible quality control index for surgical performance. Chirurgia (Bucur). 2012; 106(6):759-64. View

16.

Bolocan A, Ion D, Ciocan D, Paduraru D . Prognostic and predictive factors in colorectal cancer. Chirurgia (Bucur). 2012; 107(5):555-63. View