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Heavy-meromyosin-decoration of Microfilaments from Mougeotia Protoplasts

Overview
Journal Planta
Specialty Biology
Date 2013 Dec 6
PMID 24306883
Citations 5
Authors
K Klein
G Wagner
M R Blatt
Affiliations
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Abstract

The cell wall of the green alga Mougeotia was enzymatically digested by macerase and cellulysin. Released protoplasts were spread on poly-L-ornithine, formvar-carbon-coated grids, and cell fragments were collected for structural characterization. Large numbers of 5-7 nm filaments are seen which may be decorated with heavy meromyosin (HMM), a digest product of muscle myosin that binds specifically to actin, supporting the hypothesis that the phytochrome-mediated chloroplast movements in these algae are driven by a contractile complex of actomyosin.

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