Schiff Base Metal Derivatives Enhance the Expression of HSP70 and Suppress BAX Proteins in Prevention of Acute Gastric Lesion

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2013 Dec 4

PMID 24298554

Citations 4

Authors

Shahram Golbabapour

Nura Suleiman Gwaram

Mazen M Jamil Al-Obaidi

A F Soleimani

Hapipah Mohd Ali

Nazia Abdul Majid

Affiliations

Soon will be listed here.

Abstract

Schiff base complexes have appeared to be promising in the treatment of different diseases and disorders and have drawn a lot of attention to their biological activities. This study was conducted to evaluate the regulatory effect of Schiff base metal derivatives on the expression of heat shock proteins (HSP) 70 and BAX in protection against acute haemorrhagic gastric ulcer in rats. Rats were assigned to 6 groups of 6 rats: the normal control (Tween 20 5% v/v, 5 mL/kg), the positive control (Tween 20 5% v/v, 5 mL/kg), and four Schiff base derivative groups named Schiff_1, Schiff_2, Schiff_3, and Schiff_4 (25 mg/kg). After 1 h, all of the groups received ethanol 95% (5 mL/kg) but the normal control received Tween 20 (Tween 20 5% v/v, 5 mL/kg). The animals were euthanized after 60 min and the stomachs were dissected for histology (H&E), immunohistochemistry, and western blot analysis against HSP70 and BAX proteins. The results showed that the Schiff base metal derivatives enhanced the expression of HSP70 and suppressed the expression of BAX proteins during their gastroprotection against ethanol-induced gastric lesion in rats.

Citing Articles

In vivo acute toxicity and anti-gastric evaluation of a novel dichloro Schiff base: Bax and HSP70 alteration.

Saremi K, Rad S, Khalilzadeh M, Hussaini J, Abdul Majid N Acta Biochim Biophys Sin (Shanghai). 2020; 52(1):26-37.

PMID: 31889181 PMC: 7014572. DOI: 10.1093/abbs/gmz140.

Gastroprotective activity of a novel Schiff base derived dibromo substituted compound against ethanol-induced acute gastric lesions in rats.

Saremi K, Rad S, Tayeby F, Abdulla M, Karimian H, Abdul Majid N BMC Pharmacol Toxicol. 2019; 20(1):13.

PMID: 30770761 PMC: 6377749. DOI: 10.1186/s40360-019-0292-z.

Evidence of the gastroprotective and anti- Helicobacter pylori activities of β-mangostin isolated from Cratoxylum arborescens (vahl) blume.

Sidahmed H, Hashim N, Mohan S, Abdelwahab S, Taha M, Dehghan F Drug Des Devel Ther. 2016; 10:297-313.

PMID: 26834460 PMC: 4716727. DOI: 10.2147/DDDT.S80625.

Gastroprotective Effects of Lion's Mane Mushroom Hericium erinaceus (Bull.:Fr.) Pers. (Aphyllophoromycetideae) Extract against Ethanol-Induced Ulcer in Rats.

Wong J, Abdulla M, Raman J, Phan C, Kuppusamy U, Golbabapour S Evid Based Complement Alternat Med. 2013; 2013:492976.

PMID: 24302966 PMC: 3835629. DOI: 10.1155/2013/492976.

References

Hajrezaie M, Golbabapour S, Hassandarvish P, Gwaram N, Hadi A, Ali H . Acute toxicity and gastroprotection studies of a new schiff base derived copper (II) complex against ethanol-induced acute gastric lesions in rats. PLoS One. 2012; 7(12):e51537. PMC: 3519725. DOI: 10.1371/journal.pone.0051537. View

Strobel T, Swanson L, Korsmeyer S, Cannistra S . BAX enhances paclitaxel-induced apoptosis through a p53-independent pathway. Proc Natl Acad Sci U S A. 1996; 93(24):14094-9. PMC: 19500. DOI: 10.1073/pnas.93.24.14094. View

Khaledi H, Alhadi A, Yehye W, Ali H, Abdulla M, Hassandarvish P . Antioxidant, cytotoxic activities, and structure-activity relationship of gallic acid-based indole derivatives. Arch Pharm (Weinheim). 2011; 344(11):703-9. DOI: 10.1002/ardp.201000223. View

Jakob U, Gaestel M, Engel K, Buchner J . Small heat shock proteins are molecular chaperones. J Biol Chem. 1993; 268(3):1517-20. View

Shi Y, Thomas J . The transport of proteins into the nucleus requires the 70-kilodalton heat shock protein or its cytosolic cognate. Mol Cell Biol. 1992; 12(5):2186-92. PMC: 364390. DOI: 10.1128/mcb.12.5.2186-2192.1992. View

Cozzi P . Metal-Salen Schiff base complexes in catalysis: practical aspects. Chem Soc Rev. 2004; 33(7):410-21. DOI: 10.1039/b307853c. View

Tsukimi Y, Okabe S . Recent advances in gastrointestinal pathophysiology: role of heat shock proteins in mucosal defense and ulcer healing. Biol Pharm Bull. 2001; 24(1):1-9. DOI: 10.1248/bpb.24.1. View

Raji Y, Oyeyemi W, Shittu S, Bolarinwa A . Gastro-protective effect of methanol extract of Ficus asperifolia bark on indomethacin-induced gastric ulcer in rats. Niger J Physiol Sci. 2012; 26(1):43-8. View

Golbabapour S, Gwaram N, Hassandarvish P, Hajrezaie M, Kamalidehghan B, Abdulla M . Gastroprotection studies of Schiff base zinc (II) derivative complex against acute superficial hemorrhagic mucosal lesions in rats. PLoS One. 2013; 8(9):e75036. PMC: 3772879. DOI: 10.1371/journal.pone.0075036. View

10.

Shanker K, Rohini R, Ravinder V, Reddy P, Ho Y . Ru(II) complexes of N4 and N2O2 macrocyclic Schiff base ligands: their antibacterial and antifungal studies. Spectrochim Acta A Mol Biomol Spectrosc. 2009; 73(1):205-11. DOI: 10.1016/j.saa.2009.01.021. View

11.

Ketuly K, Hadi A, Golbabapour S, Hajrezaie M, Hassandarvish P, Ali H . Acute toxicity and gastroprotection studies with a newly synthesized steroid. PLoS One. 2013; 8(3):e59296. PMC: 3596355. DOI: 10.1371/journal.pone.0059296. View

12.

Zimmermann R . The role of molecular chaperones in protein transport into the mammalian endoplasmic reticulum. Biol Chem. 1998; 379(3):275-82. View

13.

Ismail I, Golbabapour S, Hassandarvish P, Hajrezaie M, Abdul Majid N, Kadir F . Gastroprotective Activity of Polygonum chinense Aqueous Leaf Extract on Ethanol-Induced Hemorrhagic Mucosal Lesions in Rats. Evid Based Complement Alternat Med. 2013; 2012:404012. PMC: 3544547. DOI: 10.1155/2012/404012. View

14.

Kumar K, Ganguly S, Veerasamy R, De Clercq E . Synthesis, antiviral activity and cytotoxicity evaluation of Schiff bases of some 2-phenyl quinazoline-4(3)H-ones. Eur J Med Chem. 2010; 45(11):5474-9. PMC: 7115544. DOI: 10.1016/j.ejmech.2010.07.058. View

15.

Mohan S, Abdelwahab S, Kamalidehghan B, Syam S, May K, Harmal N . Involvement of NF-κB and Bcl2/Bax signaling pathways in the apoptosis of MCF7 cells induced by a xanthone compound Pyranocycloartobiloxanthone A. Phytomedicine. 2012; 19(11):1007-15. DOI: 10.1016/j.phymed.2012.05.012. View

16.

Alam M, Choi J, Lee D . Synthesis of novel Schiff base analogues of 4-amino-1,5-dimethyl-2-phenylpyrazol-3-one and their evaluation for antioxidant and anti-inflammatory activity. Bioorg Med Chem. 2012; 20(13):4103-8. DOI: 10.1016/j.bmc.2012.04.058. View

17.

Clarke A . Molecular chaperones in protein folding and translocation. Curr Opin Struct Biol. 1996; 6(1):43-50. DOI: 10.1016/s0959-440x(96)80093-5. View

18.

Tumer M, Ekinci D, Tumer F, Bulut A . Synthesis, characterization and properties of some divalent metal(II) complexes: their electrochemical, catalytic, thermal and antimicrobial activity studies. Spectrochim Acta A Mol Biomol Spectrosc. 2006; 67(3-4):916-29. DOI: 10.1016/j.saa.2006.09.009. View

19.

Craig E, Gambill B, Nelson R . Heat shock proteins: molecular chaperones of protein biogenesis. Microbiol Rev. 1993; 57(2):402-14. PMC: 372916. DOI: 10.1128/mr.57.2.402-414.1993. View

20.

Perez Y, Oyarzabal A, Mas R, Molina V, Jimenez S . Protective effect of D-002, a mixture of beeswax alcohols, against indomethacin-induced gastric ulcers and mechanism of action. J Nat Med. 2012; 67(1):182-9. DOI: 10.1007/s11418-012-0670-y. View