Conjugated Linoleic Acid (t-10, C-12) Reduces Fatty Acid Synthesis De Novo, but Not Expression of Genes for Lipid Metabolism in Bovine Adipose Tissue Ex Vivo

Overview

Journal Lipids

Specialty Biochemistry

Date 2013 Dec 3

PMID 24293227

Citations 14

Authors

Seong Ho Choi

David T Silvey

Bradley J Johnson

Matthew E Doumit

Ki Yong Chung

Jason E Sawyer

Gwang Woong Go

Stephen B Smith

Affiliations

Soon will be listed here.

Abstract

We hypothesized that exogenous fatty acids, and especially or 18:2 trans-10, cis-12 conjugated linoleic acid (CLA), would decrease adipogenic and lipogenic gene expression and de novo fatty acid biosynthesis in intramuscular (i.m.) and subcutaneous (s.c.) adipose tissues. Fresh i.m. and s.c. adipose tissues were collected from the longissimus thoracis muscle of Angus steers at 12, 14, and 16 months of age (n = 4 per time point). Adipose tissue explants were incubated in duplicate for 48 h with 40 μM α-linolenic (ALA), oleic, stearic, trans-vaccenic, or CLA. Adipocyte size, acetate and glucose incorporation into fatty acids in vitro and mRNA levels for C/EBPβ, CPT1β, GPR43, PPARγ, PRKAA1 (AMPKα) and SCD1 were measured following the incubations. PRKAA1 and SCD1gene expression were greater (P < 0.001) in s.c. adipose tissue than in i.m. adipose tissue and acetate incorporation into lipids and C/EBPβ, PPARγ, and SCD1gene expression were greater at 16 months of age than at 12 months of age in i.m. adipose (P < 0.01). C/EBPβ gene expression increased by 16 months of age and PRKAA1 gene expression decreased by 16 months of age in s.c. adipose tissue. All fatty acids increased s.c. adipocyte volumes whereas CLA decreased acetate incorporation into lipids in s.c. adipose tissue (P < 0.05), but none of the fatty acids affected gene expression in i.m. or s.c. adipose tissue (P > 0.10). Thus, CLA depressed de novo fatty acid biosynthesis from acetate but neither CLA nor other fatty acids significantly affected adipogenic or lipogenic gene expression.

Citing Articles

Gene expression of free fatty acids-sensing G protein-coupled receptors in beef cattle.

Durand G, Charrier P, Bes S, Bernard L, Lamothe V, Gruffat D J Anim Sci. 2024; 102.

PMID: 38659415 PMC: 11075737. DOI: 10.1093/jas/skae114.

Uptake and Metabolic Conversion of Exogenous Phosphatidylcholines Depending on Their Acyl Chain Structure in .

Kotlova E, Senik S, Pozhvanov G, Prokopiev I, Boldyrev I, Manzhieva B Int J Mol Sci. 2024; 25(1).

PMID: 38203257 PMC: 10778594. DOI: 10.3390/ijms25010089.

GPR120 Gene expression and activity in subcutaneous and intramuscular adipose tissues of Angus crossbred steers.

Westbrook L, Johnson B, Gang G, Toyonaga K, Kang E, Smith S J Anim Sci. 2023; 101.

PMID: 37638631 PMC: 10601922. DOI: 10.1093/jas/skad284.

Evaluation of pineapple stem starch as a substitute for corn grain or ground cassava in a cattle feedlot for 206 or 344 days: feedlot performance, carcass characteristics, meat quality, and economic evaluation.

Khongpradit A, Boonsaen P, Homwong N, Matsuba K, Kobayashi Y, Sawanon S Trop Anim Health Prod. 2022; 54(4):226.

PMID: 35796806 DOI: 10.1007/s11250-022-03223-6.

Evidence for functional G-coupled protein receptors 43 and 120 in subcutaneous and intramuscular adipose tissue of Angus crossbred steers.

Westbrook L, Johnson B, Gang G, Toyonaga K, Hwang J, Chung K J Anim Sci. 2021; 99(6).

PMID: 33880538 PMC: 8221046. DOI: 10.1093/jas/skab125.

References

Gilmore L, Walzem R, Crouse S, Smith D, Adams T, Vaidyanathan V . Consumption of high-oleic acid ground beef increases HDL-cholesterol concentration but both high- and low-oleic acid ground beef decrease HDL particle diameter in normocholesterolemic men. J Nutr. 2011; 141(6):1188-94. DOI: 10.3945/jn.110.136085. View

Archibeque S, Lunt D, Gilbert C, Tume R, Smith S . Fatty acid indices of stearoyl-CoA desaturase do not reflect actual stearoyl-CoA desaturase enzyme activities in adipose tissues of beef steers finished with corn-, flaxseed-, or sorghum-based diets. J Anim Sci. 2005; 83(5):1153-66. DOI: 10.2527/2005.8351153x. View

Huerta-Leidenz N, Cross H, Savell J, Lunt D, Baker J, Smith S . Fatty acid composition of subcutaneous adipose tissue from male calves at different stages of growth. J Anim Sci. 1996; 74(6):1256-64. DOI: 10.2527/1996.7461256x. View

Brown A, Goldsworthy S, Barnes A, Eilert M, Tcheang L, Daniels D . The Orphan G protein-coupled receptors GPR41 and GPR43 are activated by propionate and other short chain carboxylic acids. J Biol Chem. 2002; 278(13):11312-9. DOI: 10.1074/jbc.M211609200. View

Folch J, Lees M, SLOANE STANLEY G . A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957; 226(1):497-509. View

Ekeren P, Smith D, Lunt D, Smith S . Ruminal biohydrogenation of fatty acids from high-oleate sunflower seeds. J Anim Sci. 1992; 70(8):2574-80. DOI: 10.2527/1992.7082574x. View

Janovick-Guretzky N, Dann H, Carlson D, Murphy M, Loor J, Drackley J . Housekeeping gene expression in bovine liver is affected by physiological state, feed intake, and dietary treatment. J Dairy Sci. 2007; 90(5):2246-52. DOI: 10.3168/jds.2006-640. View

Hosseini A, Sauerwein H, Mielenz M . Putative reference genes for gene expression studies in propionate and β-hydroxybutyrate treated bovine adipose tissue explants. J Anim Physiol Anim Nutr (Berl). 2010; 94(5):e178-84. DOI: 10.1111/j.1439-0396.2010.01002.x. View

Satory D, Smith S . Conjugated linoleic acid inhibits proliferation but stimulates lipid filling of murine 3T3-L1 preadipocytes. J Nutr. 1999; 129(1):92-7. DOI: 10.1093/jn/129.1.92. View

10.

Yin W, Mu J, Birnbaum M . Role of AMP-activated protein kinase in cyclic AMP-dependent lipolysis In 3T3-L1 adipocytes. J Biol Chem. 2003; 278(44):43074-80. DOI: 10.1074/jbc.M308484200. View

11.

St John L, Lunt D, Smith S . Fatty acid elongation and desaturation enzyme activities of bovine liver and subcutaneous adipose tissue microsomes. J Anim Sci. 1991; 69(3):1064-73. DOI: 10.2527/1991.6931064x. View

12.

Sessler A, Kaur N, Palta J, Ntambi J . Regulation of stearoyl-CoA desaturase 1 mRNA stability by polyunsaturated fatty acids in 3T3-L1 adipocytes. J Biol Chem. 1996; 271(47):29854-8. DOI: 10.1074/jbc.271.47.29854. View

13.

Chung K, Baxa T, Parr S, Luque L, Johnson B . Administration of estradiol, trenbolone acetate, and trenbolone acetate/estradiol implants alters adipogenic and myogenic gene expression in bovine skeletal muscle. J Anim Sci. 2012; 90(5):1421-7. DOI: 10.2527/jas.2011-3496. View

14.

Wang A, Gu Z, Heid B, Akers R, Jiang H . Identification and characterization of the bovine G protein-coupled receptor GPR41 and GPR43 genes. J Dairy Sci. 2009; 92(6):2696-705. DOI: 10.3168/jds.2009-2037. View

15.

Ge H, Li X, Weiszmann J, Wang P, Baribault H, Chen J . Activation of G protein-coupled receptor 43 in adipocytes leads to inhibition of lipolysis and suppression of plasma free fatty acids. Endocrinology. 2008; 149(9):4519-26. DOI: 10.1210/en.2008-0059. View

16.

Ortiz-Colon G, Grant A, Doumit M, Buskirk D . Bovine intramuscular, subcutaneous, and perirenal stromal-vascular cells express similar glucocorticoid receptor isoforms, but exhibit different adipogenic capacity. J Anim Sci. 2009; 87(6):1913-20. DOI: 10.2527/jas.2008-1350. View

17.

Chang J, Lunt D, Smith S . Fatty acid composition and fatty acid elongase and stearoyl-CoA desaturase activities in tissues of steers fed high oleate sunflower seed. J Nutr. 1992; 122(11):2074-80. DOI: 10.1093/jn/122.11.2074. View

18.

Saladin R, Fajas L, Dana S, Halvorsen Y, Auwerx J, Briggs M . Differential regulation of peroxisome proliferator activated receptor gamma1 (PPARgamma1) and PPARgamma2 messenger RNA expression in the early stages of adipogenesis. Cell Growth Differ. 1999; 10(1):43-8. View

19.

May S, Burney N, Wilson J, Savell J, Herring A, Lunt D . Lipogenic activity of intramuscular and subcutaneous adipose tissues from steers produced by different generations of angus sires. J Anim Sci. 1995; 73(5):1310-7. DOI: 10.2527/1995.7351310x. View

20.

Smith S, Go G, Johnson B, Chung K, Choi S, Sawyer J . Adipogenic gene expression and fatty acid composition in subcutaneous adipose tissue depots of Angus steers between 9 and 16 months of age. J Anim Sci. 2012; 90(8):2505-14. DOI: 10.2527/jas.2011-4602. View