Nucleotide Sequence of PsbC, the Gene Encoding the CP-43 Chlorophyll A-binding Protein of Photosystem II, in the Cyanobacterium Synechocystis 6803

Overview

Journal Plant Mol Biol

Date 2013 Nov 27

PMID 24277560

Citations 20

Authors

D Chisholm

J G Williams

Affiliations

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Abstract

The nucleotide sequence for the Photosystem II gene psbC has been determined for the cyanobacterium Synechocystis 6803. The gene overlaps the last 50 bases of the psbD gene, and both genes are transcribed in the same direction, but read in different frames. This arrangement is identical to that found in all chloroplast genomes for which psbC has been sequenced. The Synechocystis nucleotide sequence is 70% homologous to the tobacco gene and the predicted amino acid sequence shows 85% homology. A possible alternative translation start site for psbC has been conserved between seven plant sequences and the cyanobacterial sequence. The hydropathy plot for the cyanobacterial protein is very similar to plots determined for six plant species. Pairs of histidines that may play a role in binding chlorophyll are conserved between the cyanobacterial and plant amino acid sequences.

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References

Bookjans G, Stummann B, Rasmussen O, Henningsen K . Structure of a 3.2 kb region of pea chloroplast DNA containing the gene for the 44 kD photosystem II polypeptide. Plant Mol Biol. 2013; 6(5):359-66. DOI: 10.1007/BF00034943. View

Feinberg A, Vogelstein B . A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983; 132(1):6-13. DOI: 10.1016/0003-2697(83)90418-9. View

Rochaix J, Dron M, Rahire M, Malnoe P . Sequence homology between the 32K dalton and the D2 chloroplast membrane polypeptides of Chlamydomonas reinhardii. Plant Mol Biol. 2013; 3(6):363-70. DOI: 10.1007/BF00033383. View

Engelman D, Steitz T, Goldman A . Identifying nonpolar transbilayer helices in amino acid sequences of membrane proteins. Annu Rev Biophys Biophys Chem. 1986; 15:321-53. DOI: 10.1146/annurev.bb.15.060186.001541. View

Zurawski G, Bottomley W, Whitfeld P . Structures of the genes for the beta and epsilon subunits of spinach chloroplast ATPase indicate a dicistronic mRNA and an overlapping translation stop/start signal. Proc Natl Acad Sci U S A. 1982; 79(20):6260-4. PMC: 347100. DOI: 10.1073/pnas.79.20.6260. View

Gatenby A, Rothstein S . Synthesis of maize chloroplast ATP-synthase beta-subunit fusion proteins in Escherichia coli and binding to the inner membrane. Gene. 1986; 41(2-3):241-7. DOI: 10.1016/0378-1119(86)90104-6. View

Berends T, Gamble P, Mullet J . Characterization of the barley chloroplast transcription units containing psaA-psaB and psbD-psbC. Nucleic Acids Res. 1987; 15(13):5217-40. PMC: 305957. DOI: 10.1093/nar/15.13.5217. View

Tohdoh N, Sugiura M . The complete nucleotide sequence of 16S ribosomal RNA gene from tobacco chloroplasts. Gene. 1982; 17(2):213-8. DOI: 10.1016/0378-1119(82)90074-9. View

Vermaas W, G Williams J, Arntzen C . Sequencing and modification of psbB, the gene encoding the CP-47 protein of Photosystem II, in the cyanobacterium Synechocystis 6803. Plant Mol Biol. 2013; 8(4):317-26. DOI: 10.1007/BF00021311. View

10.

Rasmussen O, Bookjans G, Stummann B, Henningsen K . Localization and nucleotide sequence of the gene for the membrane polypeptide D2 from pea chloroplast DNA. Plant Mol Biol. 2013; 3(4):191-9. DOI: 10.1007/BF00029654. View

11.

Allen M . SIMPLE CONDITIONS FOR GROWTH OF UNICELLULAR BLUE-GREEN ALGAE ON PLATES(1, 2). J Phycol. 2016; 4(1):1-4. DOI: 10.1111/j.1529-8817.1968.tb04667.x. View

12.

Holschuh K, Bottomley W, Whitfeld P . Structure of the spinach chloroplast genes for the D2 and 44 kd reaction-centre proteins of photosystem II and for tRNASer (UGA). Nucleic Acids Res. 1984; 12(23):8819-34. PMC: 320421. DOI: 10.1093/nar/12.23.8819. View

13.

Cramer W, Theg S, Widger W . On the structure and function of cytochrome b-559. Photosynth Res. 2014; 10(3):393-403. DOI: 10.1007/BF00118305. View

14.

Shinedling S, Gayle M, Pribnow D, Gold L . Mutations affecting translation of the bacteriophage T4 rIIB gene cloned in Escherichia coli. Mol Gen Genet. 1987; 207(2-3):224-32. DOI: 10.1007/BF00331582. View

15.

Vieira J, Messing J . Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985; 33(1):103-19. DOI: 10.1016/0378-1119(85)90120-9. View

16.

KYTE J, Doolittle R . A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982; 157(1):105-32. DOI: 10.1016/0022-2836(82)90515-0. View

17.

Widger W, Cramer W, Herrmann R, Trebst A . Sequence homology and structural similarity between cytochrome b of mitochondrial complex III and the chloroplast b6-f complex: position of the cytochrome b hemes in the membrane. Proc Natl Acad Sci U S A. 1984; 81(3):674-8. PMC: 344897. DOI: 10.1073/pnas.81.3.674. View

18.

Shine J, Dalgarno L . The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci U S A. 1974; 71(4):1342-6. PMC: 388224. DOI: 10.1073/pnas.71.4.1342. View

19.

Oka A, Sugisaki H, TAKANAMI M . Nucleotide sequence of the kanamycin resistance transposon Tn903. J Mol Biol. 1981; 147(2):217-26. DOI: 10.1016/0022-2836(81)90438-1. View

20.

Pakrasi H, G Williams J, Arntzen C . Targeted mutagenesis of the psbE and psbF genes blocks photosynthetic electron transport: evidence for a functional role of cytochrome b559 in photosystem II. EMBO J. 1988; 7(2):325-32. PMC: 454321. DOI: 10.1002/j.1460-2075.1988.tb02816.x. View