APhaseolus Vulgaris Mitochondrial TRNA(Leu) is Identical to Its Cytoplasmic Counterpart: Sequencing Andin Vivo Transcription of the Gene Corresponding to the Cytoplasmic TRNA(Leu.)
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We report here that the sequence ofP. vulgaris mitochondrial and cytoplasmic tRNA(Leu) (NAA) are identical except for a post-transcriptional modification. There is an unidentified modification at the "wobble" position which, from the sequence of the nuclear tRNA(Leu) gene, we identify as a derivative of C. We also show that thisP. vulgaris nuclear gene is functional by demonstrating its transcription in anin vivo eukaryotic transcription system.
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