Schistosoma Japonicum Tegumental Protein 20.8, Role in Reproduction Through Its Calcium Binding Ability

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2013 Nov 27

PMID 24276643

Citations 4

Authors

Jia Xu

Yufeng Ren

Xue Xu

Jiajia Chen

Yuzhe Li

Wenjia Gan

Zhaoping Zhang

Hong Zhan

Xuchu Hu

Affiliations

Soon will be listed here.

Abstract

Schistosomiasis threatens thousands of millions of peoples' health every year in the world. Schistosoma japonicum, a pathogen of schistosomiasis, is covered by a lipid bilayer membrane which plays an important role in nutrient transport, signal transduction, interaction with host's immune system, etc. Thus, molecules in the tegumental membrane have gained more and more interest for understanding biological and pathological processes of schistosoma. In this study, we found a protein from S. japonicum cDNA library which has a 20.8 KDa molecular weight (SjTP20.8). Recombinant SjTP20.8 was produced and purified from Escherichia coli. The recombinant protein could be detected by S. japonicum-infected mice and human sera, and it had been found localizing in the tegumental membrane of S. japonicum in the section using immunofluorescence assay. In electrophoretic mobility shift assay, the protein could bind calcium iron in neutral condition. Result of cercariae challenge experiment indicates antibody against this protein can protect mice from chronic hepatic fibrosis. Our results indicate the S. japonicum tegumental protein 20.8 is crucial for the parasite's calcium absorbing and reproduction.

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