Overexpression of JAM-A in Non-small Cell Lung Cancer Correlates with Tumor Progression

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2013 Nov 23

PMID 24265754

Citations 35

Authors

Min Zhang

Wenting Luo

Bo Huang

Zihui Liu

Limei Sun

Qingfu Zhang

Xueshan Qiu

Ke Xu

Enhua Wang

Affiliations

Soon will be listed here.

Abstract

The objective of the current study was to determine the clinical significance of junctional adhesion molecule A (JAM-A) in patients with non-small cell lung cancer (NSCLC) and the biological function of JAM-A in NSCLC cell lines. We showed that JAM-A is predominantly expressed in cell membranes and high expression of JAM-A occurred in 37% of lung tumor specimens compared to corresponding normal tissues. High expression of JAM-A was significantly correlated with TNM stage (P = 0.021), lymph node metastasis (P = 0.007), and decreased overall survival (P = 0.02), In addition, we observed that silencing JAM-A by small interfering RNA inhibited tumor cell proliferation and induced cell cycle arrest at the G1/S boundary. Western blotting analysis revealed that knockdown of JAM-A decreased the protein levels of cyclin D1, CDK4, 6, and P-Rb. Thus, JAM-A plays an important role in NSCLC progression.

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