Rapid Detection of Human Torque Teno Viruses Using High-resolution Melting Analysis

Overview

Journal Balkan J Med Genet

Publisher Sciendo

Specialty Genetics

Date 2013 Nov 23

PMID 24265585

Citations 4

Authors

S Spandole

D Cimponeriu

M Toma

I Radu

D A Ion

Affiliations

Soon will be listed here.

Abstract

Torque teno viruses (TTVs) are recently discovered DNA viruses, with heterogeneous genomes, highly prevalent in populations worldwide. The species that infect humans are Torque teno virus (TTV), Torque teno midi virus (TTMDV) and Torque teno mini virus (TTMV). High-resolution melting analysis (HRMA) is a sensitive and effective method for genotyping and mutation scanning. Up to now, HRMA has not been utilized for detection of TTVs. The aim of this study was to asses if HRMA is suitable for detecting TTVs variants. DNA was extracted from the blood and saliva of 13 healthy subjects for method optimization. Additionally, saliva samples from 100 healthy individuals were collected for estimating the TTVs' prevalence. Viral DNA was amplified by heminested polymerase chain reaction (PCR). Second round amplicons were used for the HRMA. The samples were analyzed using two fluorescent dyes, SYBR (®) Green I and EvaGreen®. The prevalence values for TTV, TTMDV and TTMV were 71.0, 31.0 and 54.0%, respectively. The three major melting curve patterns corresponding to TTV, TTMDV and TTMV on HRMA can be easily distinguished regardless of kit used. Our results showed that HRMA is a rapid and efficient method of detecting human TTVs.

Citing Articles

Phylogenetic analysis of torque teno virus in Romania: possible evidence of distinct geographical distribution.

Spandole-Dinu S, Cimponeriu D, Stoica I, Apircioaie O, Gogianu L, Berca L Arch Virol. 2022; 167(11):2311-2318.

PMID: 35962263 PMC: 9374574. DOI: 10.1007/s00705-022-05559-8.

Prevalence of human anelloviruses in Romanian healthy subjects and patients with common pathologies.

Spandole-Dinu S, Cimponeriu D, Craciun A, Radu I, Nica S, Toma M BMC Infect Dis. 2018; 18(1):334.

PMID: 30016934 PMC: 6050727. DOI: 10.1186/s12879-018-3248-9.

Saliva and viral infections.

Corstjens P, Abrams W, Malamud D Periodontol 2000. 2015; 70(1):93-110.

PMID: 26662485 PMC: 7167623. DOI: 10.1111/prd.12112.

Prevalence of Torque teno virus in healthy donors of Paraná State, southern Brazil.

Mazzola J, Saito P, Yamakawa R, Watanabe M, Silva Junior W, Matta A Rev Bras Hematol Hemoter. 2015; 37(5):336-40.

PMID: 26408369 PMC: 4685100. DOI: 10.1016/j.bjhh.2015.07.005.

References

Wittwer C, Reed G, Gundry C, Vandersteen J, Pryor R . High-resolution genotyping by amplicon melting analysis using LCGreen. Clin Chem. 2003; 49(6 Pt 1):853-60. DOI: 10.1373/49.6.853. View

Herrmann M, Durtschi J, Wittwer C, Voelkerding K . Expanded instrument comparison of amplicon DNA melting analysis for mutation scanning and genotyping. Clin Chem. 2007; 53(8):1544-8. DOI: 10.1373/clinchem.2007.088120. View

Matsuda Y, Qazi Y, Iwaki Y . A rapid and efficient method BK polyomavirus genotyping by high-resolution melting analysis. J Med Virol. 2011; 83(12):2128-34. DOI: 10.1002/jmv.22239. View

Markham N, Zuker M . DINAMelt web server for nucleic acid melting prediction. Nucleic Acids Res. 2005; 33(Web Server issue):W577-81. PMC: 1160267. DOI: 10.1093/nar/gki591. View

Ninomiya M, Nishizawa T, Takahashi M, Lorenzo F, Shimosegawa T, Okamoto H . Identification and genomic characterization of a novel human torque teno virus of 3.2 kb. J Gen Virol. 2007; 88(Pt 7):1939-1944. DOI: 10.1099/vir.0.82895-0. View

Ruskova L, Raclavsky V . The potential of high resolution melting analysis (hrma) to streamline, facilitate and enrich routine diagnostics in medical microbiology. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2012; 155(3):239-52. DOI: 10.5507/bp.2011.045. View

Li J, Yin Y, Zheng H, Zhong M, Peng R, Wang B . A high-resolution melting analysis for genotyping urogenital Chlamydia trachomatis. Diagn Microbiol Infect Dis. 2010; 68(4):366-74. DOI: 10.1016/j.diagmicrobio.2010.07.013. View

Steer P, ORourke D, Ghorashi S, Noormohammadi A . Application of high-resolution melting curve analysis for typing of fowl adenoviruses in field cases of inclusion body hepatitis. Aust Vet J. 2011; 89(5):184-92. DOI: 10.1111/j.1751-0813.2011.00695.x. View

Toi C, Dwyer D . Prevalence of varicella-zoster virus genotypes in Australia characterized by high-resolution melt analysis and ORF22 gene analyses. J Med Microbiol. 2010; 59(Pt 8):935-940. DOI: 10.1099/jmm.0.019547-0. View

10.

Erker J, Leary T, Desai S, Chalmers M, MUSHAHWAR I . Analyses of TT virus full-length genomic sequences. J Gen Virol. 1999; 80 ( Pt 7):1743-1750. DOI: 10.1099/0022-1317-80-7-1743. View

11.

Hijikata M, Takahashi K, Mishiro S . Complete circular DNA genome of a TT virus variant (isolate name SANBAN) and 44 partial ORF2 sequences implicating a great degree of diversity beyond genotypes. Virology. 1999; 260(1):17-22. DOI: 10.1006/viro.1999.9797. View

12.

Ririe K, Rasmussen R, Wittwer C . Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal Biochem. 1997; 245(2):154-60. DOI: 10.1006/abio.1996.9916. View

13.

Robertson T, Bibby S, ORourke D, Belfiore T, Lambie H, Noormohammadi A . Characterization of Chlamydiaceae species using PCR and high resolution melt curve analysis of the 16S rRNA gene. J Appl Microbiol. 2009; 107(6):2017-28. DOI: 10.1111/j.1365-2672.2009.04388.x. View

14.

Nishizawa T, Okamoto H, Konishi K, Yoshizawa H, Miyakawa Y, Mayumi M . A novel DNA virus (TTV) associated with elevated transaminase levels in posttransfusion hepatitis of unknown etiology. Biochem Biophys Res Commun. 1998; 241(1):92-7. DOI: 10.1006/bbrc.1997.7765. View

15.

Vasilyev E, Trofimov D, Tonevitsky A, Ilinsky V, Korostin D, Rebrikov D . Torque Teno Virus (TTV) distribution in healthy Russian population. Virol J. 2009; 6:134. PMC: 2745379. DOI: 10.1186/1743-422X-6-134. View

16.

Leary T, Erker J, Chalmers M, Desai S, MUSHAHWAR I . Optimized PCR assay for the detection of TT virus. J Virol Methods. 2000; 82(2):109-12. DOI: 10.1016/s0166-0934(99)00100-7. View

17.

Zhao X, Zhou D, Wu S, Chen Y, Shao Y, Zhang J . Genotyping cytomegalovirus UL97 mutations by high-resolution melting analysis with unlabeled probe. Arch Virol. 2011; 157(3):475-81. DOI: 10.1007/s00705-011-1173-y. View

18.

Devalle S, Niel C . Distribution of TT virus genomic groups 1-5 in Brazilian blood donors, HBV carriers, and HIV-1-infected patients. J Med Virol. 2003; 72(1):166-73. DOI: 10.1002/jmv.10564. View

19.

Niel C, de Oliveira J, Ross R, Gomes S, Roggendorf M, Viazov S . High prevalence of TT virus infection in Brazilian blood donors. J Med Virol. 1999; 57(3):259-63. View

20.

Moen E, Huang L, Grinde B . Molecular epidemiology of TTV-like mini virus in Norway. Arch Virol. 2002; 147(1):181-5. DOI: 10.1007/s705-002-8310-6. View