Localization of Antigen-specific Lymphocytes Following Lymph Node Challenge

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1986 Jul 1

PMID 2426183

Citations 1

Authors

H Liu

G A Splitter

Affiliations

Soon will be listed here.

Abstract

The effect of subcutaneous injections of Brucella abortus strain 19 antigen on the specific localization of autologous lymphocytes in the regional nodes of calves was analysed by fluorescent labelling and flow cytometry. Both in vitro and in vivo FITC labelling of lymphocytes indicated the preferential migration of lymphocytes from a previously challenged lymph node to a recently challenged lymph node. However, lymphocytes from a lymph node challenged with B. abortus failed to localize preferentially in a lymph node challenged with a control antigen, Listeria monocytogenes. Lymph node cells, enriched for T lymphocytes and isolated from primary stimulated or secondary challenged B. abortus lymph nodes, could proliferate when cultured with autologous antigen-pulsed macrophages. The kinetics of [3H]thymidine incorporation in lymphocytes from secondarily challenged lymph nodes occurred earlier and to a greater extent when compared with lymphocytes from primary challenged lymph nodes. Our data show that the accumulation of B. abortus-specific lymphocytes in secondarily challenged lymph nodes is increased by the presence of the specific antigen.

Citing Articles

Isolation of Brucella abortus ssb and uvrA genes from a genomic library by use of lymphocytes as probes.

Zhu Y, Oliveira S, Splitter G Infect Immun. 1993; 61(12):5339-44.

PMID: 8225607 PMC: 281320. DOI: 10.1128/iai.61.12.5339-5344.1993.

References

UNANUE E . The regulatory role of macrophages in antigenic stimulation. Adv Immunol. 1972; 15:95-165. DOI: 10.1016/s0065-2776(08)60684-7. View

Hopkins J, McConnell I, Pearson J . Lymphocyte traffic through antigen-stimulated lymph nodes. II. Role of Prostaglandin E2 as a mediator of cell shutdown. Immunology. 1981; 42(2):225-31. PMC: 1458077. View

Hall J, Scollay R, Smith M . Studies on the lymphocytes of sheep. I. Recirculation of lymphocytes through peripheral lymph nodes and tissues. Eur J Immunol. 1976; 6(2):117-20. DOI: 10.1002/eji.1830060209. View

Young I . Proof without prejudice: use of the Kolmogorov-Smirnov test for the analysis of histograms from flow systems and other sources. J Histochem Cytochem. 1977; 25(7):935-41. DOI: 10.1177/25.7.894009. View

Sprent J . Antigen-induced selective sequestration of T lymphocytes: role of the major histocompatibility complex. Monogr Allergy. 1980; 16:233-44. View

TRNKA Z, Cahill R . Aspects of the immune response in single lymph nodes. Monogr Allergy. 1980; 16:245-59. View

Usinger W, Splitter G . A simple method for specific depletion of B lymphocytes from bovine peripheral blood mononuclear cells. Immunol Commun. 1980; 9(7):693-703. DOI: 10.3109/08820138009066018. View

Butcher E, Scollay R, Weissman I . Direct fluorescent labeling of cells with fluorescein or rhodamine isothiocyanate. II. Potential application to studies of lymphocyte migration and maturation. J Immunol Methods. 1980; 37(2):109-21. DOI: 10.1016/0022-1759(80)90196-9. View

Butcher E, Weissman I . Direct fluorescent labeling of cells with fluorescein or rhodamine isothiocyanate. I. Technical aspects. J Immunol Methods. 1980; 37(2):97-108. DOI: 10.1016/0022-1759(80)90195-7. View

10.

McConnell I, Hopkins J . Lymphocyte traffic through antigen-stimulated lymph nodes. I. Complement activation within lymph nodes initiates cell shutdown. Immunology. 1981; 42(2):217-23. PMC: 1458074. View

11.

Usinger W, Splitter G . Two molecularly independent surface receptors identify bovine T lymphocytes. J Immunol Methods. 1981; 45(3):209-19. DOI: 10.1016/0022-1759(81)90299-4. View

12.

Pabst R, Binns R . In vivo labelling of the spleen and mesenteric lymph nodes with fluorescein isothiocyanate for lymphocyte migration studies. Immunology. 1981; 44(2):321-9. PMC: 1555225. View

13.

UNANUE E . The regulatory role of macrophages in antigenic stimulation. Part Two: symbiotic relationship between lymphocytes and macrophages. Adv Immunol. 1981; 31:1-136. DOI: 10.1016/s0065-2776(08)60919-0. View

14.

Collins F, Campbell S . Immunity to intracellular bacteria. Vet Immunol Immunopathol. 1982; 3(1-2):5-66. DOI: 10.1016/0165-2427(82)90031-9. View

15.

Reichert R, Gallatin W, Weissman I, Butcher E . Germinal center B cells lack homing receptors necessary for normal lymphocyte recirculation. J Exp Med. 1983; 157(3):813-27. PMC: 2186964. DOI: 10.1084/jem.157.3.813. View

16.

Kraft R, Pauli D, Burki H, Hess M, COTTIER H, STONER R . Early changes in lymphocyte traffic between distant lymph nodes following regional antigenic stimulation. Virchows Arch B Cell Pathol Incl Mol Pathol. 1983; 44(2):135-44. DOI: 10.1007/BF02890165. View

17.

Scollay R, Chen W, Shortman K . The functional capabilities of cells leaving the thymus. J Immunol. 1984; 132(1):25-30. View

18.

Ashwell J, DeFranco A, Paul W, Schwartz R . Antigen presentation by resting B cells. Radiosensitivity of the antigen-presentation function and two distinct pathways of T cell activation. J Exp Med. 1984; 159(3):881-905. PMC: 2187250. DOI: 10.1084/jem.159.3.881. View

19.

Chin G, Cahill N . The appearance of fluorescein-labelled lymphocytes in lymph following in vitro or in vivo labelling: the route of lymphocyte recirculation through mesenteric lymph nodes. Immunology. 1984; 52(2):341-7. PMC: 1454624. View

20.

LASCELLES A, McDowell G . Localized humoral immunity with particular reference to ruminants. Transplant Rev. 1974; 19(0):170-208. DOI: 10.1111/j.1600-065x.1974.tb00132.x. View