Preventive Inositol Hexaphosphate Extracted from Rice Bran Inhibits Colorectal Cancer Through Involvement of Wnt/β-catenin and COX-2 Pathways

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2013 Nov 22

PMID 24260743

Citations 21

Authors

Nurul Husna Shafie

Norhaizan Mohd Esa

Hairuszah Ithnin

Abdah Md Akim

Norazalina Saad

Ashok Kumar Pandurangan

Affiliations

Soon will be listed here.

Abstract

Nutritional or dietary factors have drawn attention due to their potential as an effective chemopreventive agent, which is considered a more rational strategy in cancer treatment. This study was designed to evaluate the effect of IP₆ extracted from rice bran on azoxymethane- (AOM-) induced colorectal cancer (CRC) in rats. Initially, male Sprague Dawley rats were divided into 5 groups, with 6 rats in each group. The rats received two intraperitoneal (i.p.) injections of AOM in saline (15 mg/kg body weight) over a 2-week period to induce CRC. IP₆ was given in three concentrations, 0.2% (w/v), 0.5% (w/v), and 1.0% (w/v), via drinking water for 16 weeks. The deregulation of the Wnt/β-catenin signaling pathway and the expression of cyclooxygenase (COX)-2 have been implicated in colorectal tumorigenesis. β-Catenin and COX-2 expressions were analysed using the quantitative RT-PCR and Western blotting. Herein, we reported that the administration of IP₆ markedly suppressed the incidence of tumors when compared to the control. Interestingly, the administration of IP₆ had also markedly decreased β-catenin and COX-2 in colon tumors. Thus, the downregulation of β-catenin and COX-2 could play a role in inhibiting the CRC development induced by IP₆ and thereby act as a potent anticancer agent.

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References

Molenaar M, van de Wetering M, Oosterwegel M, Peterson-Maduro J, Godsave S, Korinek V . XTcf-3 transcription factor mediates beta-catenin-induced axis formation in Xenopus embryos. Cell. 1996; 86(3):391-9. DOI: 10.1016/s0092-8674(00)80112-9. View

Polakis P . The adenomatous polyposis coli (APC) tumor suppressor. Biochim Biophys Acta. 1997; 1332(3):F127-47. DOI: 10.1016/s0304-419x(97)00008-5. View

Jemal A, Bray F, Center M, Ferlay J, Ward E, Forman D . Global cancer statistics. CA Cancer J Clin. 2011; 61(2):69-90. DOI: 10.3322/caac.20107. View

Kim S, Im D, Kim S, Ryu J, Hwang S, Seong J . Beta-catenin regulates expression of cyclooxygenase-2 in articular chondrocytes. Biochem Biophys Res Commun. 2002; 296(1):221-6. DOI: 10.1016/s0006-291x(02)00824-0. View

Jariwalla R . Rice-bran products: phytonutrients with potential applications in preventive and clinical medicine. Drugs Exp Clin Res. 2001; 27(1):17-26. View

Ramezanzadeh F, Rao R, Prinyawiwatkul W, Marshall W, Windhauser M . Effects of microwave heat, packaging, and storage temperature on fatty acid and proximate compositions in rice bran. J Agric Food Chem. 2000; 48(2):464-7. DOI: 10.1021/jf9909609. View

Zullaikah S, Melwita E, Ju Y . Isolation of oryzanol from crude rice bran oil. Bioresour Technol. 2008; 100(1):299-302. DOI: 10.1016/j.biortech.2008.06.008. View

Krzystyniak K . Current strategies for anticancer chemoprevention and chemoprotection. Acta Pol Pharm. 2003; 59(6):473-8. View

He T, Sparks A, Rago C, Hermeking H, Zawel L, DA COSTA L . Identification of c-MYC as a target of the APC pathway. Science. 1998; 281(5382):1509-12. DOI: 10.1126/science.281.5382.1509. View

10.

Pandurangan A, Dharmalingam P, Ananda Sadagopan S, Ramar M, Munusamy A, Ganapasam S . Luteolin induces growth arrest in colon cancer cells through involvement of Wnt/β-catenin/GSK-3β signaling. J Environ Pathol Toxicol Oncol. 2013; 32(2):131-9. DOI: 10.1615/jenvironpatholtoxicoloncol.2013007522. View

11.

Spychalski M, Dziki L, Dziki A . Chemoprevention of colorectal cancer - a new target needed?. Colorectal Dis. 2007; 9(5):397-401. DOI: 10.1111/j.1463-1318.2006.01166.x. View

12.

Kumar A, Pandurangan A, Lu F, Fyrst H, Zhang M, Byun H . Chemopreventive sphingadienes downregulate Wnt signaling via a PP2A/Akt/GSK3β pathway in colon cancer. Carcinogenesis. 2012; 33(9):1726-35. PMC: 3514901. DOI: 10.1093/carcin/bgs174. View

13.

Cappell M . From colonic polyps to colon cancer: pathophysiology, clinical presentation, screening and colonoscopic therapy. Minerva Gastroenterol Dietol. 2007; 53(4):351-73. View

14.

Norazalina S, Norhaizan M, Hairuszah I, Norashareena M . Anticarcinogenic efficacy of phytic acid extracted from rice bran on azoxymethane-induced colon carcinogenesis in rats. Exp Toxicol Pathol. 2009; 62(3):259-68. DOI: 10.1016/j.etp.2009.04.002. View

15.

Sano H, Kawahito Y, Wilder R, Hashiramoto A, Mukai S, Asai K . Expression of cyclooxygenase-1 and -2 in human colorectal cancer. Cancer Res. 1995; 55(17):3785-9. View

16.

Pretlow T, ORiordan M, Pretlow T, Stellato T . Aberrant crypts in human colonic mucosa: putative preneoplastic lesions. J Cell Biochem Suppl. 1992; 16G:55-62. DOI: 10.1002/jcb.240501111. View

17.

Dimberg J, Hugander A, Sirsjo A, Soderkvist P . Enhanced expression of cyclooxygenase-2 and nuclear beta-catenin are related to mutations in the APC gene in human colorectal cancer. Anticancer Res. 2001; 21(2A):911-5. View

18.

Aberle H, Bauer A, Stappert J, Kispert A, Kemler R . beta-catenin is a target for the ubiquitin-proteasome pathway. EMBO J. 1997; 16(13):3797-804. PMC: 1170003. DOI: 10.1093/emboj/16.13.3797. View

19.

Rajamanickam S, Agarwal R . Natural products and colon cancer: current status and future prospects. Drug Dev Res. 2009; 69(7):460-471. PMC: 2659299. DOI: 10.1002/ddr.20276. View

20.

Morin P, Sparks A, Korinek V, Barker N, Clevers H, Vogelstein B . Activation of beta-catenin-Tcf signaling in colon cancer by mutations in beta-catenin or APC. Science. 1997; 275(5307):1787-90. DOI: 10.1126/science.275.5307.1787. View