New Method for Visualization of C-heterochromatin in Synaptonemal Complex Spreads

Overview

Journal Comp Cytogenet

Date 2013 Nov 22

PMID 24260695

Citations 1

Authors

Artem P Lisachov

Affiliations

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Abstract

DAPI staining of the metaphase chromosomes pretreated with barium hydroxide generates a C-like banding pattern. In this work a protocol for visualizing similar pattern at the synaptonemal complex (SC) spreads after immunostaining is suggested. This method was used to visualize centromeric and sex heterochromatin at the SC spreads of guppy fish (Poecilia reticulata Peters, 1859). The efficiency of this method was further confirmed at SC spreads of the northern red-backed vole (Myodes rutilus (Pallas, 1779)), the guinea pig (Cavia porcellus (Linnaeus, 1758)), and the pigmy shrew (Sorex minutus Linnaeus, 1766).

Citing Articles

Microchromosome polymorphism in the sand lizard, Linnaeus, 1758 (Reptilia, Squamata).

Lisachov A, Borodin P Comp Cytogenet. 2016; 10(3):387-399.

PMID: 27830048 PMC: 5088351. DOI: 10.3897/CompCytogen.v10i3.7655.

References

Belonogova N, Karamysheva T, Biltueva L, Perepelov E, Minina J, Polyakov A . Identification of all pachytene bivalents in the common shrew using DAPI-staining of synaptonemal complex spreads. Chromosome Res. 2006; 14(6):673-9. DOI: 10.1007/s10577-006-1079-9. View

Peters A, Plug A, van Vugt M, de Boer P . A drying-down technique for the spreading of mammalian meiocytes from the male and female germline. Chromosome Res. 1997; 5(1):66-8. DOI: 10.1023/a:1018445520117. View

Solari A . Synaptonemal complex analysis in human male infertility. Eur J Histochem. 2000; 43(4):265-76. View

Matthews H, Pearson M, Maclean N . Cat satellite DNA. Isolation using netropsin with CsCl gradients. Biochim Biophys Acta. 1980; 606(2):228-35. DOI: 10.1016/0005-2787(80)90032-5. View

Basheva E, Torgasheva A, Sakaeva G, Bidau C, Borodin P . A- and B-chromosome pairing and recombination in male meiosis of the silver fox (Vulpes vulpes L., 1758, Carnivora, Canidae). Chromosome Res. 2010; 18(6):689-96. DOI: 10.1007/s10577-010-9149-4. View

Barros e Silva A, Guerra M . The meaning of DAPI bands observed after C-banding and FISH procedures. Biotech Histochem. 2009; 85(2):115-25. DOI: 10.1080/10520290903149596. View

Nanda I, Schartl M, Epplen J, Feichtinger W, Schmid M . Primitive sex chromosomes in poeciliid fishes harbor simple repetitive DNA sequences. J Exp Zool. 1993; 265(3):301-8. DOI: 10.1002/jez.1402650311. View

Anderson L, Reeves A, Webb L, Ashley T . Distribution of crossing over on mouse synaptonemal complexes using immunofluorescent localization of MLH1 protein. Genetics. 1999; 151(4):1569-79. PMC: 1460565. DOI: 10.1093/genetics/151.4.1569. View

Heng H, Tsui L . Modes of DAPI banding and simultaneous in situ hybridization. Chromosoma. 1993; 102(5):325-32. DOI: 10.1007/BF00661275. View

10.

Santos S, Chaves R, Guedes-Pinto H . Chromosomal localization of the major satellite DNA family (FA-SAT) in the domestic cat. Cytogenet Genome Res. 2004; 107(1-2):119-22. DOI: 10.1159/000079581. View

11.

Borodin P, Karamysheva T, Belonogova N, Torgasheva A, Rubtsov N, Searle J . Recombination map of the common shrew, Sorex araneus (Eulipotyphla, Mammalia). Genetics. 2008; 178(2):621-32. PMC: 2248351. DOI: 10.1534/genetics.107.079665. View

12.

Moens P . Zebrafish: chiasmata and interference. Genome. 2006; 49(3):205-8. DOI: 10.1139/g06-021. View

13.

Basheva E, Bidau C, Borodin P . General pattern of meiotic recombination in male dogs estimated by MLH1 and RAD51 immunolocalization. Chromosome Res. 2008; 16(5):709-19. DOI: 10.1007/s10577-008-1221-y. View

14.

Del Priore L, Pigozzi M . Chromosomal axis formation and meiotic progression in chicken oocytes: a quantitative analysis. Cytogenet Genome Res. 2012; 137(1):15-21. DOI: 10.1159/000339133. View

15.

Bella J, Gosalvez J . C-banding with specific fluorescent DNA-ligands: a new approach to constitutive heterochromatin heterogeneity. Biotech Histochem. 1991; 1(1):44-52. DOI: 10.3109/10520299109110549. View