A Soluble Auxin-binding Protein from Cultured Tobacco Tissues Stimulates RNA Synthesis in Vitro

Overview

Journal Planta

Specialty Biology

Date 2013 Nov 22

PMID 24258411

Citations 9

Authors

P C van der Linde

H Bouman

A M Mennes

K R Libbenga

Affiliations

Soon will be listed here.

Abstract

When the soluble auxin receptor from tobacco callus was isolated according to H. Oostrom et al. (1975, FEBS Lett. 59, 194-197; 1980, Planta 149, 44-47) a high polyphenol contamination in the receptor preparation was observed. We developed a new isolation procedure, which drastically reduced this contamination. The receptor, which was partially purified on Sephadex G-200, exhibited the same time- and temperature-dependent binding kinetics as described before (Oostrom et al. 1975, 1980). The Ka for indole-3-acetic acid (IAA) at 25°C was about 1.6·10(8) M(-1) and the number of binding sites varied from 0 to 2·10(-13) M mg(-1) protein. Addition of partially purified receptor preparations to isolated tobaccocallus nuclei resulted in an IAA-dependent stimulation of transcription, which was not observed with similar preparations that did not contain detectable amounts of specific IAA-binding sites. The average stimulation in the presence of 1 μM IAA was 42%; it was achieved by an increase in RNA-polymerase-II activity. The stimulation was not dependent upon the presence of 1 μM IAA during the isolation of the nuclei.

Citing Articles

Auxin-induced mRNA species in tobacco cell cultures.

van der Zaal E, Memelink J, Mennes A, Quint A, Libbenga K Plant Mol Biol. 2013; 10(2):145-57.

PMID: 24277500 DOI: 10.1007/BF00016152.

Correlation between the presence of membrane-bound auxin binding and root regeneration in cultured tobacco cells.

Maan A, van der Linde P, Harkes P, Libbenga K Planta. 2013; 164(3):376-8.

PMID: 24249607 DOI: 10.1007/BF00402949.

Naphthylphthalamic acid-binding sites in cultured cells from Nicotiana tabacum.

Maan A, Kuhnel B, Beukers J, Libbenga K Planta. 2013; 164(1):69-74.

PMID: 24249501 DOI: 10.1007/BF00391027.

Marker proteins for embryogenic differentiation patterns in pea callus.

Stirn S, Jacobsen H Plant Cell Rep. 2013; 6(1):50-4.

PMID: 24248449 DOI: 10.1007/BF00269738.

Modulation of soluble auxin-binding proteins in soybean cell suspensions.

Herber B, Ulbrich B, Jacobsen H Plant Cell Rep. 2013; 7(3):178-81.

PMID: 24241593 DOI: 10.1007/BF00269317.

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