Article: Infection of cowpea mesophyll protoplasts with cowpea chlorotic mottle virus (CCMV) RNA encapsulated in large liposomes

It has been demonstrated that cowpea chlorotic mottle virus RNA encapsulated in phosphatidyl serine/cholesterol reverse evaporation vesicles (REV) could infect cowpea mesophyll protoplasts under conditions known to enhance liposome-protoplast interactions. Positively charged phosphatidylcholine/stearylamine multilamellar liposomes did not deliver functional CCMV RNA despite their very high nucleic acid trapping capacity and their high affinity for protoplasts.

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PMID: 24253684 DOI: 10.1007/BF00269206.

References

1.

Larkin P . Purification and viability determinations of plant protoplasts. Planta. 2014; 128(3):213-6. DOI: 10.1007/BF00393231. View

2.

Pick U . Liposomes with a large trapping capacity prepared by freezing and thawing of sonicated phospholipid mixtures. Arch Biochem Biophys. 1981; 212(1):186-94. DOI: 10.1016/0003-9861(81)90358-1. View

3.

Lurquin P . Entrapment of plasmid DNA by liposomes and their interactions with plant protoplasts. Nucleic Acids Res. 1979; 6(12):3773-84. PMC: 327977. DOI: 10.1093/nar/6.12.3773. View

4.

Beier H, Bruening G . The use of an abrasive in the isolation of cowpea leaf protoplasts which support the multiplication of cowpea mosaic virus. Virology. 1975; 64(1):272-6. DOI: 10.1016/0042-6822(75)90099-9. View

5.

Uchimiya H, Harada H . Transfer of Liposome-Sequestering Plasmid DNA into Daucus carota Protoplasts. Plant Physiol. 1981; 68(5):1027-30. PMC: 426039. DOI: 10.1104/pp.68.5.1027. View

6.

Szoka Jr F, Papahadjopoulos D . Procedure for preparation of liposomes with large internal aqueous space and high capture by reverse-phase evaporation. Proc Natl Acad Sci U S A. 1978; 75(9):4194-8. PMC: 336078. DOI: 10.1073/pnas.75.9.4194. View

7.

Kao K, Michayluk M . A method for high-frequency intergeneric fusion of plant protoplasts. Planta. 2014; 115(4):355-67. DOI: 10.1007/BF00388618. View

8.

Fukunaga Y, Nagata T, Takebe I . Liposome-mediated infection of plant protoplasts with tobacco mosaic virus RNA. Virology. 1981; 113(2):752-60. DOI: 10.1016/0042-6822(81)90203-8. View

9.

Fraley R, Dellaporta S, Papahadjopoulos D . Liposome-mediated delivery of tobacco mosaic virus RNA into tobacco protoplasts: A sensitive assay for monitoring liposome-protoplast interactions. Proc Natl Acad Sci U S A. 1982; 79(6):1859-63. PMC: 346080. DOI: 10.1073/pnas.79.6.1859. View

10.

Otsuki Y, Takebe I . Fluorescent antibody staining of tobacco mosaic virus antigen in tobacco mesophyll protoplasts. Virology. 1969; 38(3):497-9. DOI: 10.1016/0042-6822(69)90167-6. View

11.

Keller W, Melchers G . The effect of high pH and calcium on tobacco leaf protoplast fusion. Z Naturforsch C. 1973; 28(11):737-41. DOI: 10.1515/znc-1973-11-1215. View

12.

Matthews B, Cress D . Liposome-mediated delivery of DNA to carrot protoplasts. Planta. 2013; 153(1):90-4. DOI: 10.1007/BF00385322. View

Infection of Cowpea Mesophyll Protoplasts with Cowpea Chlorotic Mottle Virus (CCMV) RNA Encapsulated in Large Liposomes