Molecular Cloning and Characterisation of CDNAs Complementary to MRNAs from Wounded Potato (Solanum Tuberosum) Tuber Tissue

Overview

Journal Planta

Specialty Biology

Date 2013 Nov 21

PMID 24253170

Citations 6

Authors

A D Shirras

D H Northcote

Affiliations

Soon will be listed here.

Abstract

Five cDNA clones complementary to mRNAs representing different abundances and responses to wounding have been isolated from a library of Sau 3A fragments in the bacteriophage M13 mp8. These were characterised by hybrid-release translation and hybridisation to RNA blots. The levels of RNA complementary to two of the clones show a marked increase during the 24h after wounding, one shows a small increase and two show no appreciable changes except that caused by a general increase in the total amount of polyadenylated RNA per microgram of total RNA which increases 2.5-fold during the same period. The would-induced RNAs are not induced in diluted suspension-culture cells, but RNA complementary to each clone is present in varying levels in stems, leaves and roots of intact potato plants.

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