Cross-reactivity of Monoclonal Antibodies Against Lipopolysaccharides of Gram-negative Bacteria

Overview

Journal Eur J Clin Microbiol

Specialty Microbiology

Date 1986 Apr 1

PMID 2424754

Citations 3

Authors

A S Bouter

J H Marcelis

J Schellekens

J Verhoef

Affiliations

Soon will be listed here.

Abstract

Monoclonal antibodies were produced against Escherichia coli O111, Escherichia coli J5, and the rough (R) mutant of Salmonella typhimurium M206, and tested by enzyme-linked immunosorbent assay against lipopolysaccharides of several gram-negative strains. The monoclonal antibodies were also identified with an immunoblotting assay. Anti-Escherichia coli O111 monoclonal antibodies reacted only with homologous O antigens. Anti-J5 monoclonal antibodies cross-reacted with core lipopolysaccharide, especially with Rc lipopolysaccharide. IgM anti-J5 monoclonal antibodies showed more extensive cross-reactivity than IgG3 monoclonal antibodies. Anti-Re monoclonal antibodies cross-reacted weakly with all rough lipopolysaccharide tested. Thus, the varying specificity of these monoclonal antibodies seems to indicate that the core regions in the lipopolysaccharides of various gram-negative bacteria are not similar.

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Carumonam enhances reactivity of Escherichia coli with mono- and polyclonal antisera to rough Escherichia coli J5.

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Characterization of anti-core glycolipid monoclonal antibodies with chemically defined lipopolysaccharides.

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