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Monoclonal Antibodies to Plant Growth Regulators. II. Indole-3-acetic Acid

Overview
Journal Planta
Specialty Biology
Date 2013 Nov 19
PMID 24241522
Citations 9
Authors
R Mertens
J Eberle
A Arnscheidt
A Ledebur
E W Weiler
Affiliations
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Abstract

The production and characterization of high-affinity monoclonal antibodies suitable for the radio- and enzymeimmunoassay of the endogenous plant growth regulator, indole-3-acetic acid (IAA), is reported. Hybridomas were produced by fusion of NS 1 myeloma cells with spleen cells from Balb/c mice immunized with IAA-bovine serum albumin conjugates. From an initial collection of 158 wells containing cells secreting monoclonal antibodies against IAA, seven were used to derive cell clones. Three of these are described here. They secrete immunoglobulin (IgG2a or IgG2b) of high affinity and specificity for IAA methyl ester and can be used to quantite picogram amounts of this compound in plant extracts by radio- and enzymeimmunoassay.

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References
1.
Mertens R, Weiler E . Kinetic studies on the redistribution of endogenous growth regulators in gravireacting plant organs. Planta. 2013; 158(4):339-48. DOI: 10.1007/BF00397336. View
2.
Weiler E . Radioimmunoassay for pmol-quantities of indole-3-acetic acid for use with highly stable [(125)I]- and [ (3)H]IAA derivatives as radiotracers. Planta. 2013; 153(4):319-25. DOI: 10.1007/BF00384250. View
3.
Bandurski R, Schulze A . Concentrations of Indole-3-acetic Acid and Its Esters in Avena and Zea. Plant Physiol. 1974; 54(3):257-62. PMC: 367393. DOI: 10.1104/pp.54.3.257. View
4.
Skoog F, Miller C . Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symp Soc Exp Biol. 1957; 11:118-30. View
5.
Schroder G, Waffenschmidt S, Weiler E, Schroder J . The T-region of Ti plasmids codes for an enzyme synthesizing indole-3-acetic acid. Eur J Biochem. 1984; 138(2):387-91. DOI: 10.1111/j.1432-1033.1984.tb07927.x. View